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Article

Oxidative/antioxidative enzyme-mediated antiproliferative and proapoptotic effects of the GPER1 agonist G-1 on lung cancer cells

  • Authors:
    • Akif Hakan Kurt
    • Ahmet Çelik
    • Bekir Mehmet Kelleci
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, Faculty of Medicine, Kahramanmaras Sutcu Imam University, Kahramanmaras, Turkey, Department of Biochemistry, Faculty of Medicine, Kahramanmaras Sutcu Imam University, Kahramanmaras, Turkey, Department of Biology, Faculty of Science and Art, Kahramanmaras Sutcu Imam University, Kahramanmaras, Turkey
  • Pages: 3177-3182
    |
    Published online on: September 16, 2015
       https://doi.org/10.3892/ol.2015.3711
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Abstract

Estrogen mediates fast signal responses or transcriptional events via G protein-coupled estrogen receptor 1 (GPER1). However, there is no data on the effect of GPER1 on lung cancer cell proliferation and apoptosis. The present study aimed to analyze the anticancer effects of the GPER1 agonist G‑1 on A549 human lung cancer cells. A549 cells were treated with 17β‑estradiol and G‑1, and cell proliferation was analyzed using MTT and WST assays. In addition, the apoptotic effects induced by G‑1 were investigated using acridine orange/ethidium bromide staining. A549 cells were treated with a half maximal inhibitory concentration of G‑1 for 72 h, and nitric oxide (NO) levels and superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx) enzyme activities were analyzed by spectrophotometry. The results revealed that G‑1 significantly decreased cell proliferation. In addition to the antiprolif­erative effect of G‑1, a marked increase in apoptotic activity was observed when cells were treated with 2x10‑5 M G‑1. Furthermore, G‑1 increased NO levels, and SOD and GPx activity. These findings indicate that the GPER1 agonist G‑1 is able to exert antiproliferative and proapoptotic effects on A549 cells, and that oxidant and antioxidant molecules may mediate these effects.
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Copy and paste a formatted citation
Spandidos Publications style
Kurt AH, Çelik A and Kelleci BM: Oxidative/antioxidative enzyme-mediated antiproliferative and proapoptotic effects of the GPER1 agonist G-1 on lung cancer cells. Oncol Lett 10: 3177-3182, 2015.
APA
Kurt, A.H., Çelik, A., & Kelleci, B.M. (2015). Oxidative/antioxidative enzyme-mediated antiproliferative and proapoptotic effects of the GPER1 agonist G-1 on lung cancer cells. Oncology Letters, 10, 3177-3182. https://doi.org/10.3892/ol.2015.3711
MLA
Kurt, A. H., Çelik, A., Kelleci, B. M."Oxidative/antioxidative enzyme-mediated antiproliferative and proapoptotic effects of the GPER1 agonist G-1 on lung cancer cells". Oncology Letters 10.5 (2015): 3177-3182.
Chicago
Kurt, A. H., Çelik, A., Kelleci, B. M."Oxidative/antioxidative enzyme-mediated antiproliferative and proapoptotic effects of the GPER1 agonist G-1 on lung cancer cells". Oncology Letters 10, no. 5 (2015): 3177-3182. https://doi.org/10.3892/ol.2015.3711
Copy and paste a formatted citation
x
Spandidos Publications style
Kurt AH, Çelik A and Kelleci BM: Oxidative/antioxidative enzyme-mediated antiproliferative and proapoptotic effects of the GPER1 agonist G-1 on lung cancer cells. Oncol Lett 10: 3177-3182, 2015.
APA
Kurt, A.H., Çelik, A., & Kelleci, B.M. (2015). Oxidative/antioxidative enzyme-mediated antiproliferative and proapoptotic effects of the GPER1 agonist G-1 on lung cancer cells. Oncology Letters, 10, 3177-3182. https://doi.org/10.3892/ol.2015.3711
MLA
Kurt, A. H., Çelik, A., Kelleci, B. M."Oxidative/antioxidative enzyme-mediated antiproliferative and proapoptotic effects of the GPER1 agonist G-1 on lung cancer cells". Oncology Letters 10.5 (2015): 3177-3182.
Chicago
Kurt, A. H., Çelik, A., Kelleci, B. M."Oxidative/antioxidative enzyme-mediated antiproliferative and proapoptotic effects of the GPER1 agonist G-1 on lung cancer cells". Oncology Letters 10, no. 5 (2015): 3177-3182. https://doi.org/10.3892/ol.2015.3711
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