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Article

Correlation between p65 and TNF-α in patients with acute myelocytic leukemia

  • Authors:
    • Qiao‑Mei Dong
    • Chun Ling
    • Jun‑Fang Zhu
    • Xuan Chen
    • Yan Tang
    • Li Zhao
  • View Affiliations / Copyright

    Affiliations: Central Laboratory, The First Hospital of Lanzhou University, Lanzhou, Gansu 730000, P.R. China
  • Pages: 3305-3309
    |
    Published online on: September 17, 2015
       https://doi.org/10.3892/ol.2015.3720
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Abstract

The correlation between the expression levels of p65 and TNF-α in patients with acute myelocytic leukemia (AML) and AML cell lines were investigated. The bone marrow samples of 30 AML patients and 10 non‑leukemia controls were studied. The mRNA expression levels of p65 and TNF‑α were detected by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR), and Pearson's Correlation test was used to demonstrate the correlation between TNFα and p65 expression levels in AML specimens. Receiver operating characteristic (ROC) curves were plotted to determine whether TNFα and p65 expression levels could be used to differentiate AML samples from non‑leukemia samples. MG132 and anti‑TNFα antibody were used to inhibit the expression of p65 and TNF‑α in the AML cell line, HL‑60. The expression of p65 and TNF‑α were detected by RT‑qPCR and western blot analysis. The mRNA expression levels of p65 and TNF‑α were significantly increased in AML patients compared with non‑leukemia control bone marrow samples by RT‑qPCR, and the two molecules expression pattern's exhibited sufficient predictive power to distinguish AML patients from non‑leukemia control samples. Pearson's correlation analysis demonstrated that TNFα expression was strongly correlated with p65 expression in AML bone marrow samples. In HL‑60 cells, inhibition of TNFα reduced the expression of p65; in addition, inhibition of p65 reduced the expression of TNFα as assessed by RT‑qPCR and western blot analysis. p65 and TNF‑α were highly expressed in AML patients, and these 2 molecules were strongly correlated. The present study indicates that p65 and TNF‑α have potential as molecular markers to distinguish AML patients from non-leukemia control samples, and that these 2 molecules may be useful prognostic factor for patients with AML.
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Copy and paste a formatted citation
Spandidos Publications style
Dong QM, Ling C, Zhu JF, Chen X, Tang Y and Zhao L: Correlation between p65 and TNF-α in patients with acute myelocytic leukemia. Oncol Lett 10: 3305-3309, 2015.
APA
Dong, Q., Ling, C., Zhu, J., Chen, X., Tang, Y., & Zhao, L. (2015). Correlation between p65 and TNF-α in patients with acute myelocytic leukemia. Oncology Letters, 10, 3305-3309. https://doi.org/10.3892/ol.2015.3720
MLA
Dong, Q., Ling, C., Zhu, J., Chen, X., Tang, Y., Zhao, L."Correlation between p65 and TNF-α in patients with acute myelocytic leukemia". Oncology Letters 10.5 (2015): 3305-3309.
Chicago
Dong, Q., Ling, C., Zhu, J., Chen, X., Tang, Y., Zhao, L."Correlation between p65 and TNF-α in patients with acute myelocytic leukemia". Oncology Letters 10, no. 5 (2015): 3305-3309. https://doi.org/10.3892/ol.2015.3720
Copy and paste a formatted citation
x
Spandidos Publications style
Dong QM, Ling C, Zhu JF, Chen X, Tang Y and Zhao L: Correlation between p65 and TNF-α in patients with acute myelocytic leukemia. Oncol Lett 10: 3305-3309, 2015.
APA
Dong, Q., Ling, C., Zhu, J., Chen, X., Tang, Y., & Zhao, L. (2015). Correlation between p65 and TNF-α in patients with acute myelocytic leukemia. Oncology Letters, 10, 3305-3309. https://doi.org/10.3892/ol.2015.3720
MLA
Dong, Q., Ling, C., Zhu, J., Chen, X., Tang, Y., Zhao, L."Correlation between p65 and TNF-α in patients with acute myelocytic leukemia". Oncology Letters 10.5 (2015): 3305-3309.
Chicago
Dong, Q., Ling, C., Zhu, J., Chen, X., Tang, Y., Zhao, L."Correlation between p65 and TNF-α in patients with acute myelocytic leukemia". Oncology Letters 10, no. 5 (2015): 3305-3309. https://doi.org/10.3892/ol.2015.3720
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