Low expression of spindle checkpoint protein, Cenp-E, causes numerical chromosomal abnormalities in HepG-2 human hepatoma cell

Liu,Bin; Luo,Hongmei; Wu,Guang; Liu,Jing; Pan,Jie; Liu,Zhuoran

doi:10.3892/ol.2015.3721

November-2015 Volume 10 Issue 5

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November-2015 Volume 10 Issue 5

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Article Open Access

Low expression of spindle checkpoint protein, Cenp-E, causes numerical chromosomal abnormalities in HepG-2 human hepatoma cell

Authors:
- Bin Liu
- Hongmei Luo
- Guang Wu
- Jing Liu
- Jie Pan
- Zhuoran Liu
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Affiliations: Department of Laboratory Medicine, The Second Affiliated Hospital, University of South China, Hengyang, Hunan 421001, P.R. China, Department of Histology and Embryology, University of South China, Hengyang, Hunan 421001, P.R. China

Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 2699-2704
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Published online on: September 18, 2015

https://doi.org/10.3892/ol.2015.3721
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Abstract

The aim of the present study was to investigate the expression, localization and role of centromere-associated protein E (Cenp-E) in hepatoma cells. The Cenp-E mRNA expression levels in the HepG-2 human hepatocellular carcinoma and LO2 normal hepatic cell lines following treatment with nocodazole were detected by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). Furthermore, the localization and expression of Cenp‑E protein in the two cell types was visualized using indirect immunofluorescence. RT‑qPCR was also performed to detect the Cenp‑E mRNA expression levels in LO2 cells before and after RNA interference. Additional evaluation of the function of interfered cells was performed using indirect immunofluorescence. The results of RT‑qPCR demonstrated that the protein expression levels of Cenp‑E in the two cell lines prior to treatment with nocodazole were not significantly different (P>0.05). However, the upregulation of Cenp‑E expression levels in the LO2 cells was significantly higher compared with that in the HepG‑2 cells during cell division (P<0.05). Indirect immunofluorescence analysis indicated that the Cenp‑E protein was predominantly located in the nucleus, and that Cenp‑E protein expression in nuclei with abnormal mitosis was markedly lower compared with that in nuclei exhibiting normal mitosis. Indirect immunofluorescence also determined that the ratio of dyskaryosis was significantly higher in cells that had undergone Cenp‑E interference compared with normal cells. Thus, the present study indicated that the low expression of Cenp‑E mRNA may be an important reason for numerical chromosomal abnormalities in human hepatoma cells.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Low expression of spindle checkpoint protein, Cenp-E, causes numerical chromosomal abnormalities in HepG-2 human hepatoma cell

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