Knockdown of heme oxygenase-1 promotes apoptosis and autophagy and enhances the cytotoxicity of doxorubicin in breast cancer cells

  • Authors:
    • Xiao-Feng Zhu
    • Wen Li
    • Jie-Yi Ma
    • Nan Shao
    • Yun-Jian Zhang
    • Rui-Ming Liu
    • Wei-Bin Wu
    • Ying Lin
    • Shen-Ming Wang
  • View Affiliations

  • Published online on: September 21, 2015     https://doi.org/10.3892/ol.2015.3735
  • Pages: 2974-2980
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Abstract

Heme oxygenase-1 (HMOX-1) is a microsomal enzyme that exerts anti-apoptotic and cytoprotective effects. In the present study, HMOX-1 was demonstrated to be overexpressed and able to be induced by doxorubicin in breast cancer cell lines. Knockdown of HMOX-1 using short interfering (si)RNA enhanced the cytotoxicity of doxorubicin in MDA-MB‑231 and BT549 cells. Knockdown of HMOX-1 downregulated B cell lymphoma (Bcl)-2 and Bcl-extra large expression, and significantly enhanced doxorubicin-induced apoptosis in MDA-MB-231 and BT549 cells. Additionally, knockdown of HMOX-1 upregulated light chain 3B expression and markedly increased the accumulation of autophagic vacuoles in MDA-MB-231 and BT549 cells treated with doxorubicin. These results indicated that HMOX-1 may be involved in conferring the chemoresistance of breast cancer cells, by preventing apoptosis and autophagy. Therefore, HMOX-1 may represent a potential therapeutic target for enhancing the cytotoxicity and efficacy of doxorubicin during the treatment of breast cancer.
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November-2015
Volume 10 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhu X, Li W, Ma J, Shao N, Zhang Y, Liu R, Wu W, Lin Y and Wang S: Knockdown of heme oxygenase-1 promotes apoptosis and autophagy and enhances the cytotoxicity of doxorubicin in breast cancer cells. Oncol Lett 10: 2974-2980, 2015
APA
Zhu, X., Li, W., Ma, J., Shao, N., Zhang, Y., Liu, R. ... Wang, S. (2015). Knockdown of heme oxygenase-1 promotes apoptosis and autophagy and enhances the cytotoxicity of doxorubicin in breast cancer cells. Oncology Letters, 10, 2974-2980. https://doi.org/10.3892/ol.2015.3735
MLA
Zhu, X., Li, W., Ma, J., Shao, N., Zhang, Y., Liu, R., Wu, W., Lin, Y., Wang, S."Knockdown of heme oxygenase-1 promotes apoptosis and autophagy and enhances the cytotoxicity of doxorubicin in breast cancer cells". Oncology Letters 10.5 (2015): 2974-2980.
Chicago
Zhu, X., Li, W., Ma, J., Shao, N., Zhang, Y., Liu, R., Wu, W., Lin, Y., Wang, S."Knockdown of heme oxygenase-1 promotes apoptosis and autophagy and enhances the cytotoxicity of doxorubicin in breast cancer cells". Oncology Letters 10, no. 5 (2015): 2974-2980. https://doi.org/10.3892/ol.2015.3735