Comparison of immunohistochemistry and mRNA in situ hybridization in detecting thyroid transcription factor-1 expression in non-small cell lung carcinomas tissue

  • Authors:
    • Huiyong Jiang
    • Xiaoyan Bai
    • Fanjun Meng
    • Xuefeng Zhang
  • View Affiliations

  • Published online on: September 29, 2015     https://doi.org/10.3892/ol.2015.3757
  • Pages: 3581-3584
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Thyroid transcription factor-1 (TTF-1) reportedly possesses oncogenic and suppressive roles within the same tumor type and may play a dual function in the progression of lung cancer. Immunohistochemistry (IHC) and mRNA in situ hybridization (ISH) are commonly used methods for detecting protein or mRNA expression. The present study compared the concordance rate of the two methods in the evaluation of thyroid transcription factor‑1 (TTF‑1) expression in non‑small cell lung carcinoma (NSCLC) using tissue microarray‑based IHC and mRNA ISH. TTF‑1 protein and mRNA expression levels were examined in 196 cases of NSCLC. The IHC and mRNA ISH agreement was 91.3% (179/196), and near‑perfect agreement was observed between the two methods (κ‑coefficient, 0.848). There was no significant difference between IHC and mRNA ISH, as analyzed by the McNemar‑Bowker test (P=0.219). The present findings proved that IHC is comparable to mRNA ISH for evaluating TTF‑1 expression in NSCLC. These two methods can be used to detect TTF-1 expression in future studies.
View Figures
View References

Related Articles

Journal Cover

December-2015
Volume 10 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Jiang H, Bai X, Meng F and Zhang X: Comparison of immunohistochemistry and mRNA in situ hybridization in detecting thyroid transcription factor-1 expression in non-small cell lung carcinomas tissue. Oncol Lett 10: 3581-3584, 2015
APA
Jiang, H., Bai, X., Meng, F., & Zhang, X. (2015). Comparison of immunohistochemistry and mRNA in situ hybridization in detecting thyroid transcription factor-1 expression in non-small cell lung carcinomas tissue. Oncology Letters, 10, 3581-3584. https://doi.org/10.3892/ol.2015.3757
MLA
Jiang, H., Bai, X., Meng, F., Zhang, X."Comparison of immunohistochemistry and mRNA in situ hybridization in detecting thyroid transcription factor-1 expression in non-small cell lung carcinomas tissue". Oncology Letters 10.6 (2015): 3581-3584.
Chicago
Jiang, H., Bai, X., Meng, F., Zhang, X."Comparison of immunohistochemistry and mRNA in situ hybridization in detecting thyroid transcription factor-1 expression in non-small cell lung carcinomas tissue". Oncology Letters 10, no. 6 (2015): 3581-3584. https://doi.org/10.3892/ol.2015.3757