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Article Open Access

Establishment and characterization of intraperitoneal xenograft models by co‑injection of human tumor cells and extracellular matrix gel

  • Authors:
    • Yuqin Yao
    • Yongjun Zhou
    • Xiaolan Su
    • Lei Dai
    • Lin Yu
    • Hongxin Deng
    • Lantu Gou
    • Jinliang Yang
  • View Affiliations / Copyright

    Affiliations: Research Centre for Public Health and Preventive Medicine, West China School of Public Health, No. 4 West China Teaching Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China, State Key Laboratory of Biotherapy and Cancer Center, Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China
    Copyright: © Yao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3450-3456
    |
    Published online on: September 29, 2015
       https://doi.org/10.3892/ol.2015.3764
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Abstract

Establishing a feasible intraperitoneal (i.p.) xenograft model in nude mice is a good strategy to evaluate the antitumor effect of drugs in vivo. However, the manipulation of human cancer cells in establishing a stable peritoneal carcinomatosis model in nude mice is problematic. In the present study, the ovarian and colorectal peritoneal tumor models were successfully established in nude mice by co‑injection of human tumor cells and extracellular matrix gel. In ovarian tumor models, the mean number tumor nodes was significantly higher in the experimental group (intraperitoneal tumor cell co‑injection with ECM gel) compared with the PBS control group on the 30th day (21.0±3.0 vs. 3.6±2.5; P<0.05). The same results were observed in the colorectal peritoneal tumor models on the 28th day. The colorectal peritoneal tumor model was further used to evaluate the chemotherapy effect of irinotecan (CPT‑11). The mean weight of peritoneal tumor nodes in CPT‑11 treatment group was significantly less than that of the control group (0.81±0.16 vs. 2.18±0.21 g; P<0.05). The results confirmed the value of these i.p. xenograft models in nude mice as efficient and feasible tools for preclinical evaluation.
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Copy and paste a formatted citation
Spandidos Publications style
Yao Y, Zhou Y, Su X, Dai L, Yu L, Deng H, Gou L and Yang J: Establishment and characterization of intraperitoneal xenograft models by co‑injection of human tumor cells and extracellular matrix gel. Oncol Lett 10: 3450-3456, 2015.
APA
Yao, Y., Zhou, Y., Su, X., Dai, L., Yu, L., Deng, H. ... Yang, J. (2015). Establishment and characterization of intraperitoneal xenograft models by co‑injection of human tumor cells and extracellular matrix gel. Oncology Letters, 10, 3450-3456. https://doi.org/10.3892/ol.2015.3764
MLA
Yao, Y., Zhou, Y., Su, X., Dai, L., Yu, L., Deng, H., Gou, L., Yang, J."Establishment and characterization of intraperitoneal xenograft models by co‑injection of human tumor cells and extracellular matrix gel". Oncology Letters 10.6 (2015): 3450-3456.
Chicago
Yao, Y., Zhou, Y., Su, X., Dai, L., Yu, L., Deng, H., Gou, L., Yang, J."Establishment and characterization of intraperitoneal xenograft models by co‑injection of human tumor cells and extracellular matrix gel". Oncology Letters 10, no. 6 (2015): 3450-3456. https://doi.org/10.3892/ol.2015.3764
Copy and paste a formatted citation
x
Spandidos Publications style
Yao Y, Zhou Y, Su X, Dai L, Yu L, Deng H, Gou L and Yang J: Establishment and characterization of intraperitoneal xenograft models by co‑injection of human tumor cells and extracellular matrix gel. Oncol Lett 10: 3450-3456, 2015.
APA
Yao, Y., Zhou, Y., Su, X., Dai, L., Yu, L., Deng, H. ... Yang, J. (2015). Establishment and characterization of intraperitoneal xenograft models by co‑injection of human tumor cells and extracellular matrix gel. Oncology Letters, 10, 3450-3456. https://doi.org/10.3892/ol.2015.3764
MLA
Yao, Y., Zhou, Y., Su, X., Dai, L., Yu, L., Deng, H., Gou, L., Yang, J."Establishment and characterization of intraperitoneal xenograft models by co‑injection of human tumor cells and extracellular matrix gel". Oncology Letters 10.6 (2015): 3450-3456.
Chicago
Yao, Y., Zhou, Y., Su, X., Dai, L., Yu, L., Deng, H., Gou, L., Yang, J."Establishment and characterization of intraperitoneal xenograft models by co‑injection of human tumor cells and extracellular matrix gel". Oncology Letters 10, no. 6 (2015): 3450-3456. https://doi.org/10.3892/ol.2015.3764
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