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Increased activity of CHK enhances the radioresistance of MCF-7 breast cancer stem cells

  • Authors:
    • Zhi‑Xue Yang
    • Yi‑Hui Sun
    • Jian‑Gang He
    • Hua Cao
    • Guo‑Qin Jiang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3443-3449
    |
    Published online on: October 2, 2015
       https://doi.org/10.3892/ol.2015.3777
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Abstract

The resistance of breast cancer to radiotherapy remains a major obstacle to successful cancer management. Radiotherapy may result in DNA damage and activate breast cancer stem cells. DNA damage may lead to activation of the checkpoint kinase (CHK) signaling pathway, of which debromohymenialdisine (DBH) is a specific inhibitor. Radiotherapy also increases the expression of phosphorylated CHK1/2 (pCHK1/2) in the breast cancer cell line, MCF‑7, in vitro in a dose‑dependent manner. DBH is a relatively stable effective inhibitor that significantly reduces pCHK1/2 expression and MCF‑7 proliferation. Low‑dose radiotherapy combined with DBH resulted in a higher MCF‑7 inhibition rate compared with high‑dose radiation alone. This result indicates that the inhibition of the CHK1/2 signal pathway may significantly reduce DNA damage within radiated cells. Radiotherapy may also regulate the proportion of CD44+/CD24‑ MCF‑7 cancer stem cells in a dose‑ and time‑dependent manner. However, the stem cell proportion of MCF‑7 cells was significantly reduced by treatment with DBH. The inhibition is relatively stable and time dependent. Significant reductions were observed after 3 days of culture (P<0.01). The results of the present study indicate that the DBH‑induced downregulation of CHK may provide a novel method of enhancing the effect of radiotherapy and reducing stem cell survival in the MCF-7 cell line.
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Copy and paste a formatted citation
Spandidos Publications style
Yang ZX, Sun YH, He JG, Cao H and Jiang GQ: Increased activity of CHK enhances the radioresistance of MCF-7 breast cancer stem cells. Oncol Lett 10: 3443-3449, 2015.
APA
Yang, Z., Sun, Y., He, J., Cao, H., & Jiang, G. (2015). Increased activity of CHK enhances the radioresistance of MCF-7 breast cancer stem cells. Oncology Letters, 10, 3443-3449. https://doi.org/10.3892/ol.2015.3777
MLA
Yang, Z., Sun, Y., He, J., Cao, H., Jiang, G."Increased activity of CHK enhances the radioresistance of MCF-7 breast cancer stem cells". Oncology Letters 10.6 (2015): 3443-3449.
Chicago
Yang, Z., Sun, Y., He, J., Cao, H., Jiang, G."Increased activity of CHK enhances the radioresistance of MCF-7 breast cancer stem cells". Oncology Letters 10, no. 6 (2015): 3443-3449. https://doi.org/10.3892/ol.2015.3777
Copy and paste a formatted citation
x
Spandidos Publications style
Yang ZX, Sun YH, He JG, Cao H and Jiang GQ: Increased activity of CHK enhances the radioresistance of MCF-7 breast cancer stem cells. Oncol Lett 10: 3443-3449, 2015.
APA
Yang, Z., Sun, Y., He, J., Cao, H., & Jiang, G. (2015). Increased activity of CHK enhances the radioresistance of MCF-7 breast cancer stem cells. Oncology Letters, 10, 3443-3449. https://doi.org/10.3892/ol.2015.3777
MLA
Yang, Z., Sun, Y., He, J., Cao, H., Jiang, G."Increased activity of CHK enhances the radioresistance of MCF-7 breast cancer stem cells". Oncology Letters 10.6 (2015): 3443-3449.
Chicago
Yang, Z., Sun, Y., He, J., Cao, H., Jiang, G."Increased activity of CHK enhances the radioresistance of MCF-7 breast cancer stem cells". Oncology Letters 10, no. 6 (2015): 3443-3449. https://doi.org/10.3892/ol.2015.3777
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