Study of the mechanism underlying the inhibitory effects of transglutaminase Ⅱ on apoptosis in the osteosarcoma MG-63 cell line under hypoxic conditions

Wang,Guobin; Fu,Limei; Chen,Fangmin

doi:10.3892/ol.2015.3778

December-2015 Volume 10 Issue 6

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December-2015 Volume 10 Issue 6

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Study of the mechanism underlying the inhibitory effects of transglutaminase Ⅱ on apoptosis in the osteosarcoma MG-63 cell line under hypoxic conditions

Authors:
- Guobin Wang
- Limei Fu
- Fangmin Chen
View Affiliations / Copyright

Affiliations: Department of Bone Surgery, Binzhou People's Hospital, Binzhou, Shandong, P.R. China, Department of Pathology, Binzhou People's Hospital, Binzhou, Shandong, P.R. China, Department of Orthopedics, Affiliated Hospital of Binzhou Medical College, Binzhou, Shandong, P.R. China

Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 3425-3428
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Published online on: October 2, 2015

https://doi.org/10.3892/ol.2015.3778
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Abstract

The aim of the present study was to investigate the association between the apoptosis phenomenon in the MG-63 osteosarcoma cell line, and transglutaminase Ⅱ (TG2) expression. The relationship between the anti‑apoptotic mechanism of TG2 and the expression of cytochrome c as well as caspase‑3 under hypoxic conditions was also verified. A hypoxic culture of MG‑63 cells was prepared. The hypoxia and TG2 siRNA hypoxia groups were established, and the cultures were incubated for 12 h under hypoxic conditions. TG2 activity, TG2 protein expression and its mRNA level were investigated. Cytochrome c and caspase‑3 protein levels in the TG2 nucleus and cytoplasm were measured. The apoptotic rate was also monitored. The results showed that TG2 activity, TG2 protein expression and its mRNA level in the hypoxia group were significantly higher than those of the siRNA hypoxia group. The results showed statistically insignificant differences (P<0.05). By contrast, a comparison of the two groups in the cytoplasm yielded no statistically significant differences (P>0.05). Cytochrome c and caspase‑3 protein levels in the hypoxia group were significantly higher than those of the TG2 siRNA hypoxia group. The results showed statistically significant differences (P<0.05). By contrast, the protein levels in the cytoplasm were significantly lower than those of the TG2 siRNA hypoxia group, with differences being statistically significant (P<0.05). The differences in apoptotic rates between the hypoxia and TG2 siRNA hypoxia groups were also statistically significant (P<0.05). Under hypoxic conditions, a high TG2 expression inhibited the apoptosis of the MG‑63 osteosarcoma cell line. This effect was probably associated with its suppressive activity on the transportation of cytochrome c and caspase-3 from nucleus to cytoplasm.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Study of the mechanism underlying the inhibitory effects of transglutaminase Ⅱ on apoptosis in the osteosarcoma MG-63 cell line under hypoxic conditions

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