Tyrphostin AG 1296 induces glioblastoma cell apoptosis in vitro and in vivo

Introduction

Glioblastoma multiforme (GBM) is the most prevalent type of primary human brain tumor, and has low rates of survival (1–3). Current therapeutic strategies for GBM, including radiotherapy and chemotherapy, do not produce satisfactory clinical outcomes (4,5). Therefore, a novel and effective approach with which to treat GBM is required. Mechanistic studies suggest that numerous genetic and physiological alterations occur in GBM, which affect the survival and proliferation of glioblastoma cells (6). Receptor tyrosine kinases (RTKs) are genes/proteins that are frequently modified during the pathogenesis of GBM (7). Evidence from a number of studies, supports the hypothesis that platelet-derived growth factor receptor (PDGFR), a member of the type III RTK family, and the signaling pathway through PDGFR is strongly associated with the development of GBM (8–10). PDGF and its receptors have been shown to be upregulated in one third of surgical glioblastoma samples and human glioblastoma cell lines (8). In addition, in vitro and in vivo studies have demonstrated that PDGF and its receptors are involved in the proliferation, cell migration and angiogenesis of GBM cells (11,12). Furthermore, PDGF autocrine loops have been reported to be involved in the tumorigenesis of GBM (13,14). In light of these findings, it was hypothesized that inhibition of PDGF signaling may be a viable therapeutic approach for GBM.

Currently, different types of PDGF antagonists are being developed and evaluated in animal disease models, in addition to clinical trials (15). Tyrphostin AG 1296 is a specific inhibitor of human PDGFR-α and −#x03B2;, as well as of c-KIT (also termed sCD117), fms-related tyrosine kinase 3 and Bek (a fibroblast growth factor receptor) tyrosine kinases (16–19). It has been reported that tyrphostin AG 1296 inhibits cell proliferation and promotes apoptosis in small cell lung cancer, leukemia and anaplastic thyroid cancer (20–22). Recently, it has been suggested that AG 1296 may suppress the proliferation and migration of glioblastoma cells (23,24). Whilst tyrphostin AG 1296 has been shown to affect the proliferation and migration of glioblastoma cells, its effect on the rate of apoptosis in GBM remains unclear.

In the present study, the effects of tyrphostin AG 1296 on cell apoptosis in glioblastoma cells were examined in vitro and in vivo. The in vitro experiments demonstrated that tyrphostin AG 1296 reduced cell viability, inhibited cell migration and promoted cell apoptosis. The in vivo experiments also showed that tyrphostin AG 1296 inhibited tumor growth and induced tumor cell apoptosis in a mouse xenograft model of human GBM. Therefore the current data support the hypothesis that tyrphostin AG 1296 inhibits the growth of glioblastoma cells by inducing apoptosis.

Materials and methods

U87MG cell culture

U87MG, a representative grade IV glioma cell line, was obtained from the American Type Culture Collection (Manassas, VA, USA). U87MG cells were cultured in Dulbecco's modified Eagle's medium/F12/10% FBS medium (Invitrogen, Grand Island, NY, USA). Cells were maintained in a humidified incubator at 37°C with 5% CO2.

Cell viability assay

U87MG Cells were treated with 0.3125, 0.625, 1.25, 2.5, 5, 10 or 20 µM of tyrphostin AG 1296 (ThermoFisher Scientific, Inc., Waltham, MA USA; Catalog No. 50-230-7882), 72 h after cells had been seeded in 96-well plates for a period of 24 h. In order to assess whether the duration of treatment affected cell viability, cells were treated with 5 mM tyrphostin AG 1296 for 24, 48 or 72 h. The cell viability assay was performed using a CellTiter-Glo® Assay kit (Promega Corporation, Madison, WI USA). Experiments were repeated three times, independently.

Propidium iodide analysis

Cells were treated with tyrphostin AG 1296 at various concentrations and harvested after 48 h. Propidium iodide staining was performed using a Cycletest™ plus DNA reagent kit (Becton Dickinson, San Jose, CA, USA), followed by detection of the apoptotic cells with DNA content in sub-G1, using a FACSCalibur flow cytometry. Data were analyzed using CellQuest Pro software, version 5.1 (Becton Dickinson).

Morphological analysis of U87MG cells

U87MG cells treated with tyrphostin AG 1296 at various concentrations, were stained using Hoechst. Cells with condensed or fragmented nuclei were identified as apoptotic cells using a Olympus BX53 microscope (Olympus Corporation, Tokyo, Japan).

Cell invasion assay

For the cell invasion assay, 1×106 cells were plated into the upper chambers of 24-transwell Boyden chamber wells (Costar, Bedford, MA, USA), and treated with tyrphostin AG 1296 at various concentrations for 8 hr. Following treatment, cells were fixed and stained with 0.1% crystal violet for imaging. Migrated cells were lysed with 10% acetic acid following imaging. The absorbance of lysate was measured at 595 nm by using a Nanodrop 2000 (Thermo Scientific, Inc., Wilmongton, DE, USA).

Tyrphostin AG 1296 treatment in a mouse xenograft model in vivo

Approval for the use of mice in the present study, was obtained from the Animal Ethics Committee and the Institutional Review Board of Wuhan University (Wuhan, China). C57BL/6J mice were purchased from Shanghai SLAC laboratory Animal Co., Ltd. (Shanhai, China). Animals were provided free access to food and water and were housed in a 13:11 (light:dark) cycle. Female mice aged 6–8 weeks, weighing 20 g were used for experiments. U87MG cells (2×106 cells/mouse) were injected into the axillary regions of mice. When tumor volumes had reached 70 mm3, the mice were randomly divided into a control group and low and high dose tyrphostin AG 1296 treatment groups (n=6/group). Low-dose (50 mg/kg) or high-dose (100 mg/kg) tyrphostin AG 1296, or vehicle (10% 1-methyl-2-pyrrolidinone and 90% polyethylene glycol 300) was intravenously injected into the mice each day for 13 days. Tumor size and body weight were measured daily. The tumor volume was calculated according to the formula: Volume (mm3) = (width × width × length)/2.

Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay

Tumor samples from the control and the AG 1296-treatment groups were harvested from mice following 13 days of treatment. The tumors were cut into 5-mm3 sections and fixed in 4% paraformaldehyde. Subsequently, samples were embedded in paraffin blocks and 5-mm sections were produced. Apoptotic cells were identified using a TUNEL assay. Briefly, tumor tissues were incubated with TdT enzyme solution (New England Biolabs, Inc., Ipswich, MA, USA) for 60 min, washed twice with PBS twice and then incubated for 30 min at 25°C with DAPI. Images were captured using an Olympus BX53 fluorescence microscope.

Statistical analysis

All experiments were repeated in triplicates, independently. The data were analyzed by a one-way or two-wayanalysis of variance followed by Tukey's post-hoc test. Graphs were produced using SPSS statistical software, version 17.0 (SPSS, Chicago, IL, USA). Data are expressed as the mean ± standard deviation. P<0.05 was considered to indicate a statistically significant difference.

Results

Tyrphostin AG 1296 inhibits U87MG cell viability

Previous studies have suggested that tyrphostin AG 1296, an inhibitor of PDGFR, reduces glioblastoma cell proliferation (24). In order to confirm whether tyrphostin AG 1296 affects glioblastoma cell growth, a cell viability assay was performed in U87MG cells. The results showed that tyrphostin AG 1296 inhibited the viability of U87MG cells, in a dose-dependent manner (Fig. 1A). It was observed that cell viability was almost fully suppressed when cells were treated with 20 µM tyrphostin AG 1296 for 72 h (Fig. 1A). In order to investigate whether the effect of tyrphostin AG 1296 on the viability of U87MG cells is time-dependent, cells were treated with 5 µM tyrphostin AG 1296 for 24, 48 and 72 h. It was shown tyrphostin AG 1296 reduced U87MG cell viability in a time-dependent manner (Fig. 1B). Therefore, the current data confirm that tyrphostin AG 1296 inhibits glioblastoma cell viability in vitro.

Figure 1. Tyrphostin AG 1296 decreased cell viability of U87MG cells. (A) Cell viability was measured following treatment of U87MG cells with tyrphostin AG 1296 (0.3125–20 µM) for 72 h. (B) Cell viability was measured following treatment of U87MG cells with tyrphostin AG 1296 (5 µM) for 24, 48 or 72 h. The viability of cells was set to 100% in the untreated control group.*P<0.05 vs. control.

Tyrphostin AG 1296 inhibits U87MG cell invasion in vitro

Subsequently, the effects of tyrphostin AG 1296 on U87MG cell invasion were examined. Matrigel invasion assays, with 8.0 µm-pore transwells were used. The results of the invasion assays showed that low and high concentrations of tyrphostin AG 1296 effectively restricted U87MG cell migration across the filter (Fig. 2A). Statistical analysis demonstrated that the anti-migration effect of tyrphostin AG 1296 in U87MG cells, increased in a dose-dependent manner (Fig. 2B). These results are in accordance with those of a previous study, which suggested that tyrphostin AG 1296 inhibits glioblastoma cell invasion in vitro (23).

Figure 2. Tyrphostin AG 1296 inhibited cell migration of glioblastoma cells. (A) Migrated cells on the lower side were stained and photographed following treatment of U87MG cells with tyrphostin AG 1296 (12.5, 50 or 200 nM) for 8 h. Representative images are shown. The dark cells are migrated cells stained with crystal violet. Less dark cells are present in the tyrphostin AG-treated groups compared with the control group. (B) Quantitative analysis of inhibition of cell invasion, as assessed using a transwell assay. Inhibition was set to 100% for the untreated control group. *P<0.05 vs. control.

Tyrphostin AG 1296 induces apoptosis of U87MG cells

Although the effects of tyrphostin AG 1296 on cell growth and migration have been investigated, its effect on glioblastoma cell apoptosis has not been fully elucidated. In order to investigate whether tyrphostin AG 1296 regulates cell apoptosis, the fraction of apoptotic cells with sub-G1 DNA content, with and without tyrphostin AG 1296 treatment, was examined. The results showed that the percentage of cells with sub-G1 DNA content increased in a dose-dependent manner (Fig. 3A). Furthermore, U87MG cells exposed to tyrphostin AG 1296 exhibited the condensed and fragmented morphology (Fig. 3B), which is characteristic of apoptotic cells. The number of cells with condensed or fragmented nuclei was markedly increased in U87MG cells, and this increase occurred in a dose-dependent manner (Fig. 3C). These results demonstrated that tyrphostin AG 1296 induces apoptosis in U87MG cells.

Figure 3. Tyrphostin AG 1296 promoted cell apoptosis in U87MG cells. (A) Flow cytometry analysis of apoptotic cells following treatment of U87MG cells with tyrphostin AG 1296 (0, 2.5, 5, 10 or 20 µM) for 48 h. (B) U87MG cells were incubated with tyrphostin AG 1296 for 48 h and then stained with Hoechst. Images were captures using a fluorescence microscope and representative images are shown. (C) Quantitative analysis of the number of cells with condensed/fragmented nuclei observed in seven randomly-selected fields. Cell inhibition was set to 100% in the untreated control group.*P<0.05 vs. control.

Tyrphostin AG 1296 antagonizes tumor growth and induces apoptosis of tumor cells in vivo

In order to measure the antitumor activity of tyrphostin AG 1296 in vivo, a glioblastoma xenograft model was generated by injecting mice with U87MG cells. Once tumor volumes reached 70 mm3, various doses of tyrphostin AG 1296 were administered over 13 days. Compared with the control group, tyrphostin AG 1296 led to reduction of tumor growth at low (50 mg/kg) and high (100 mg/kg) doses. Furthermore, high-dose tyrphostin AG 1296 suppressed tumor growth to a greater extent than low-dose treatment (Fig. 4A). In accordance with the in vitro data, cell apoptosis was observed in tumor tissues (Fig. 4B). Tyrphostin AG 1296 induced changes characteristic of apoptosis, as measured using a TUNEL assay (Fig. 4B). In addition, mice exhibited no significant signs of toxicity or weight loss during Tyrphostin AG 1296 treatment (data not shown). These data indicate that tyrphostin AG 1296 suppresses GBM tumor growth by inducing cell apoptosis in vivo.

Figure 4. Tyrphostin AG 1296 repressed glioblastoma tumor growth in vivo. (A) Tyrphostin AG 1296 (50 and 100 mg/kg) was injected into mice daily, for a period of 13 days following inoculation of U87MG cells. Tumor volumes were measured on alternate days and the growth curves of tumors are shown. *P<0.05 vs. control. (B) The effect of tyrphostin AG 1296 on U87MG cell apoptosis in vivo was measured using a TUNEL assay (green). Nuclei were stained with Hoechst (blue). TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling.

Discussion

GBM is the most common type of primary tumor of the central nervous system (25–27). The overall median survival period is ~15 months for individuals with GBM (28). The majority of current chemotherapeutic approaches for GBM involve targeting tyrosine kinase receptor signaling pathways, due to the central role of these molecules in the oncogenesis of GBM (29). However, RTK inhibitors have not produced the level of efficacy in patients with GBM, which had been anticipated (30,31). Thus, the development of novel chemotherapeutic agents for GBM is required. The present study examined the effect of tyrphostin AG 1296 on glioblastoma cell growth and migration in vitro and in vivo. In accordance with previous studies (23,24), tyrphostin AG 1296 exhibited marked efficacy in the inhibition of glioblastoma cell growth and migration in vitro. Furthermore, it was demonstrated that tyrphostin AG 1296 induced cell apoptosis in vitro and in vivo. The inhibitory effects of tyrphostin AG 1296 on proliferation and migration, and the induction of apoptosis by this agent, resulted in a reduction in the growth of GBM tumors in vivo (Fig. 4). The results of previous studies and of the present study, on tyrphostin AG 1296 improve the current understanding of GBM treatment and suggest a novel therapeutic approach to this disease.

In addition to tyrphostin AG 1296, other inhibitors targeting PDGFR-β, include Imatinib. Imatinib is currently used for the clinical treatment of certain types of cancer, including chronic myeloid leukemia and gastrointestinal stromal tumor (32–34). However, Imatinib has exhibited limited efficacy in patients with GBM in phase I/II clinical trials (35), which may be a result of resistance, due to mutations in the GBM tumors (36,37). The present study demonstrated a strong antitumor activity of tyrphostin AG 1296 in a glioblastoma cell line and a GBM mouse model. Therefore, tyrphostin AG 1296 may be a promising chemotherapeutic option for malignant GBM. However, its efficacy in patients with GBM requires further evaluation.

Acknowledgements

The authors would like to thank Professor Xiwen Shi (Henan Hospital, Zhengzhou, China), Professor Lijun Huang (Xinxiang Hospital, Xinxiang, China) and Professor Cheng Wang (Shenzhen hospital, Shenzen, China) for their advice, as well as all members of the laboratory for their technical support and suggestions.

References