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Article Open Access

Aggressive invasion is observed in CD133‑/A2B5+ glioma-initiating cells

  • Authors:
    • Ting Sun
    • Guilin Chen
    • Yanyan Li
    • Xueshun Xie
    • Youxin Zhou
    • Ziwei Du
  • View Affiliations / Copyright

    Affiliations: Neurosurgery and Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China
    Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3399-3406
    |
    Published online on: October 20, 2015
       https://doi.org/10.3892/ol.2015.3823
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Abstract

Glioblastoma multiforme is the most common and fatal primary brain tumor in adults. Aggressive invasion of glioblastoma cells into brain tissue often limits complete surgical resection and contributes to therapeutic resistance. The cell surface marker, CD133, has been identified as a putative stem cell marker in normal and malignant brain tissues; CD133‑/A2B5+ cells exhibit neural stem‑like cell properties. The invasive properties and the molecular mechanisms of CD133‑/A2B5+ glioma‑initiating cells (GICs) were investigated in the process of self‑renewal and tumorigenesis. An increased number of invasive cells through matrigel and an increase in migratory cells through filters were observed in CD133‑/A2B5+ GIC populations compared with matched non‑initiating tumor cell populations. Considerable changes were detected in expression of mRNA and protein associated with migration or invasion. CD133‑/A2B5+ GIC demonstrated infiltrating growth patterns and displayed greater invasive potential under fluorescent microscopy comparing with the matched non‑initiating tumor cells after cells labeled with red fluorescence protein were transplanted into the brains of athymic/nude mice. CD133‑/A2B5+ GICs possess strong migratory and invasive capacity. These infiltrating cells in the invasive fronts may be responsible for rapid tumor recurrence following conventional treatments. CD133‑/A2B5+ GICs may be an important subpopulation with high invasive potential and they should not be ignored when targeting GICs to prevent GBM recurrence.
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1 

Stupp R, Mason WP, van den Bent MJ, Weller M, Fisher B, Taphoorn MJ, Belanger K, Brandes AA, Marosi C, Bogdahn U, et al: Radiotherapy plus concomitant and adjuvant temozolomide for glioblastoma. N Engl J Med. 352:987–996. 2005. View Article : Google Scholar : PubMed/NCBI

2 

Vehlow A and Cordes N: Invasion as target for therapy of glioblastoma multiforme. Biochim Biophys Acta. 1836:236–244. 2013.PubMed/NCBI

3 

Singh SK, Hawkins C, Clarke ID, Squire JA, Bayani J, Hide T, Henkelman RM, Cusimano MD and Dirks PB: Identification of human brain tumour initiating cells. Nature. 432:396–401. 2004. View Article : Google Scholar : PubMed/NCBI

4 

Galli R, Binda E, Orfanelli U, Cipelletti B, Gritti A, De Vitis S, Fiocco R, Foroni C, Dimeco F and Vescovi A: Isolation and characterization of tumorigenic, stem-like neural precursors from human glioblastoma. Cancer Res. 64:7011–7021. 2004. View Article : Google Scholar : PubMed/NCBI

5 

Bao S, Wu Q, McLendon RE, Hao Y, Shi Q, Hjelmeland AB, Dewhirst MW, Bigner DD and Rich JN: Glioma stem cells promote radioresistance by preferential activation of the DNA damage response. Nature. 444:756–760. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Sanai N, Alvarez-Buylla A and Berger MS: Neural stem cells and the origin of gliomas. N Engl J Med. 353:811–822. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Li Z, Bao S, Wu Q, Wang H, Eyler C, Sathornsumetee S, Shi Q, Cao Y, Lathia J, McLendon RE, et al: Hypoxia-inducible factors regulate tumorigenic capacity of glioma stem cells. Cancer Cell. 15:501–513. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Lee J, Kotliarova S, Kotliarov Y, Li A, Su Q, Donin NM, Pastorino S, Purow BW, Christopher N, Zhang W, et al: Tumor stem cells derived from glioblastomas cultured in bFGF and EGF more closely mirror the phenotype and genotype of primary tumors than do serum-cultured cell lines. Cancer Cell. 9:391–403. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Singh SK, Clarke ID, Terasaki M, Bonn VE, Hawkins C, Squire J and Dirks PB: Identification of a cancer stem cell in human brain tumors. Cancer Res. 63:5821–5828. 2003.PubMed/NCBI

10 

Brescia P, Ortensi B, Fornasari L, Levi D, Broggi G and Pelicci G: CD133 is essential for glioblastoma stem cell maintenance. Stem Cells. 31:857–869. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Colin C, Baeza N, Tong S, Bouvier C, Quilichini B, Durbec P and Figarella-Branger D: In vitro identification and functional characterization of glial precursor cells in human gliomas. Neuropathol Appl Neurobiol. 32:189–202. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Nunes MC, Roy NS, Keyoung HM, Goodman RR, McKhann G II, Jiang L, Kang J, Nedergaard M and Goldman SA: Identification and isolation of multipotential neural progenitor cells from the subcortical white matter of the adult human brain. Nat Med. 9:439–447. 2003. View Article : Google Scholar : PubMed/NCBI

13 

Miconi G, Palumbo P, Dehcordi SR, La Torre C, Lombardi F, Evtoski Z, Cimini AM, Galzio R, Cifone MG and Cinque B: Immunophenotypic characterization of human glioblastoma stem cells: Correlation with clinical outcome. J Cell Biochem. 116:864–876. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Stieber D, Golebiewska A, Evers L, Lenkiewicz E, Brons NH, Nicot N, Oudin A, Bougnaud S, Hertel F, Bjerkvig R, et al: Glioblastomas are composed of genetically divergent clones with distinct tumourigenic potential and variable stem cell-associated phenotypes. Acta Neuropathol. 127:203–219. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Son MJ, Woolard K, Nam DH, Lee J and Fine HA: SSEA-1 is an enrichment marker for tumor-initiating cells in human glioblastoma. Cell Stem Cell. 4:440–452. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Lathia JD, Gallagher J, Heddleston JM, Wang J, Eyler CE, Macswords J, Wu Q, Vasanji A, McLendon RE, Hjelmeland AB, et al: Integrin alpha 6 regulates glioblastoma stem cells. Cell Stem Cell. 6:421–432. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Visvader JE and Lindeman GJ: Cancer stem cells: Current status and evolving complexities. Cell Stem Cell. 10:717–728. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Ogden AT, Waziri AE, Lochhead RA, Fusco D, Lopez K, Ellis JA, Kang J, Assanah M, McKhann GM, Sisti MB, et al: Identification of A2B5+CD133- tumor-initiating cells in adult human gliomas. Neurosurgery. 62:505–515. 2008. View Article : Google Scholar : PubMed/NCBI

19 

Tchoghandjian A, Baeza N, Colin C, Cayre M, Metellus P, Beclin C, Ouafik L and Figarella-Branger D: A2B5 cells from human glioblastoma have cancer stem cell properties. Brain Pathol. 20:211–221. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Friedl P and Alexander S: Cancer invasion and the microenvironment: Plasticity and reciprocity. Cell. 147:992–1009. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Farahani E, Patra HK, Jangamreddy JR, Rashedi I, Kawalec M, Rao Pariti RK, Batakis P and Wiechec E: Cell adhesion molecules and their relation to (cancer) cell stemness. Carcinogenesis. 35:747–759. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Bourboulia D and Stetler-Stevenson WG: Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs): Positive and negative regulators in tumor cell adhesion. Semin Cancer Biol. 20:161–168. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Kessenbrock K, Plaks V and Werb Z: Matrix metalloproteinases: Regulators of the tumor microenvironment. Cell. 141:52–67. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Yamamoto N, Tsuchiya H and Hoffman RM: Tumor imaging with multicolor fluorescent protein expression. Int J Clin Oncol. 16:84–91. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Zhang X, Zheng X, Jiang F, Zhang ZG, Katakowski M and Chopp M: Dual-color fluorescence imaging in a nude mouse orthotopic glioma model. J Neurosci Methods. 181:178–185. 2009. View Article : Google Scholar : PubMed/NCBI

26 

Beier D, Hau P, Proescholdt M, Lohmeier A, Wischhusen J, Oefner PJ, Aigner L, Brawanski A, Bogdahn U and Beier CP: CD133(+) and CD133(−) glioblastoma-derived cancer stem cells show differential growth characteristics and molecular profiles. Cancer Res. 67:4010–4015. 2007. View Article : Google Scholar : PubMed/NCBI

27 

Joo KM, Kim SY, Jin X, Song SY, Kong DS, Lee JI, Jeon JW, Kim MH, Kang BG, Jung Y, et al: Clinical and biological implications of CD133-positive and CD133-negative cells in glioblastomas. Lab Invest. 88:808–815. 2008. View Article : Google Scholar : PubMed/NCBI

28 

Wang J, Sakariassen PØ, Tsinkalovsky O, Immervoll H, Bøe SO, Svendsen A, Prestegarden L, Røsland G, Thorsen F, Stuhr L, et al: CD133 negative glioma cells form tumors in nude rats and give rise to CD133 positive cells. Int J Cancer. 122:761–768. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Sun T, Chen G, Li Y, Xie X, Zhou Y and Du Z: Aggressive invasion is observed in CD133‑/A2B5+ glioma-initiating cells. Oncol Lett 10: 3399-3406, 2015.
APA
Sun, T., Chen, G., Li, Y., Xie, X., Zhou, Y., & Du, Z. (2015). Aggressive invasion is observed in CD133‑/A2B5+ glioma-initiating cells. Oncology Letters, 10, 3399-3406. https://doi.org/10.3892/ol.2015.3823
MLA
Sun, T., Chen, G., Li, Y., Xie, X., Zhou, Y., Du, Z."Aggressive invasion is observed in CD133‑/A2B5+ glioma-initiating cells". Oncology Letters 10.6 (2015): 3399-3406.
Chicago
Sun, T., Chen, G., Li, Y., Xie, X., Zhou, Y., Du, Z."Aggressive invasion is observed in CD133‑/A2B5+ glioma-initiating cells". Oncology Letters 10, no. 6 (2015): 3399-3406. https://doi.org/10.3892/ol.2015.3823
Copy and paste a formatted citation
x
Spandidos Publications style
Sun T, Chen G, Li Y, Xie X, Zhou Y and Du Z: Aggressive invasion is observed in CD133‑/A2B5+ glioma-initiating cells. Oncol Lett 10: 3399-3406, 2015.
APA
Sun, T., Chen, G., Li, Y., Xie, X., Zhou, Y., & Du, Z. (2015). Aggressive invasion is observed in CD133‑/A2B5+ glioma-initiating cells. Oncology Letters, 10, 3399-3406. https://doi.org/10.3892/ol.2015.3823
MLA
Sun, T., Chen, G., Li, Y., Xie, X., Zhou, Y., Du, Z."Aggressive invasion is observed in CD133‑/A2B5+ glioma-initiating cells". Oncology Letters 10.6 (2015): 3399-3406.
Chicago
Sun, T., Chen, G., Li, Y., Xie, X., Zhou, Y., Du, Z."Aggressive invasion is observed in CD133‑/A2B5+ glioma-initiating cells". Oncology Letters 10, no. 6 (2015): 3399-3406. https://doi.org/10.3892/ol.2015.3823
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