Differential effect of psoralidin in enhancing apoptosis of colon cancer cells via nuclear factor‑κB and B‑cell lymphoma‑2/B‑cell lymphoma‑2‑associated X protein signaling pathways

Jin,Zhiliang; Yan,Wei; Jin,Hui; Ge,Changzheng; Xu,Yanhua

doi:10.3892/ol.2015.3861

Differential effect of psoralidin in enhancing apoptosis of colon cancer cells via nuclear factor‑κB and B‑cell lymphoma‑2/B‑cell lymphoma‑2‑associated X protein signaling pathways

Authors:
- Zhiliang Jin
- Wei Yan
- Hui Jin
- Changzheng Ge
- Yanhua Xu
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Affiliations: Department of Oncology, Central Hospital of Jingzhou, Jingzhou, Hubei 434020, P.R. China, Department of Gastroenterology, Central Hospital of Jingzhou, Jingzhou, Hubei 434020, P.R. China, Department of Neonatology, Central Hospital of Jingzhou, Jingzhou, Hubei 434020, P.R. China
Published online on: November 4, 2015 https://doi.org/10.3892/ol.2015.3861
Pages: 267-272

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Abstract

Worldwide, colon cancer is the third most common cancer in terms of incidence, following lung and breast cancer. Resistance to psoralidin frequently occurs following its use as an anticancer treatment. However, the mechanisms underlying the effects of psoralidin on colon cancer, remain to be elucidated. Hence, the present study investigated the anticancer effects and potential mechanism of action of psoralidin on SW480 human colon cancer cells. In the present study, an MTT assay was performed to measure the viability of SW480 cells. Additionally, an Annexin V‑fluorescein isothiocyanate/propidium iodide apoptosis detection kit, DAPI staining assay and caspase‑3 colorimetric assay kits were used to analyze the cellular apoptosis of SW480 cells. The nuclear factor‑κB (NF‑κB) p65 activity and B‑cell lymphoma‑2 (Bcl‑2)/Bcl‑2‑associated X protein (Bax) protein expression of SW480 cells was detected using NF‑κB colorimetric assay kits and western blot analysis, respectively. Bcl‑2 inhibitor ABT‑737 was added to SW480 cells and the subsequent effects and mechanism of action of psoralidin on SW480 colon cancer cells was studied. In the present study, psoralidin reduced SW480 cell viability and enhanced the cellular apoptosis of SW480 cells in a dose‑dependent manner. Caspase‑3 activity of SW480 cells was increased following treatment with psoralidin. Additionally, psoralidin was able to reduce the NF‑κB p65 activity of SW480 cells. Furthermore, psoralidin was able to reduce Bcl‑2 protein expression and increase Bax protein expression in SW480 cells. Notably, Bcl‑2 inhibitor was observed to enhance the effects of psoralidin on SW480 cells. The results of the present study suggest that psoralidin may be a candidate drug for the treatment of colon cancer by inhibition of the NF‑κB and Bcl‑2/Bax signaling pathways.

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1	Isik A, Peker K, Firat D, Yilmaz B, Sayar I, Idiz O, Cakir C, Demiryilmaz I and Yilmaz I: Importance of metastatic lymph node ratio in non-metastatic, lymph node-invaded colon cancer: A clinical trial. Med Sci Monit. 20:1369–1375. 2014. View Article : Google Scholar : PubMed/NCBI
2	Lu ZJ, Lu LG, Tao KZ, Chen DF, Xia Q, Weng JJ, Zhu F, Wang XP and Zheng P: MicroRNA-185 suppresses growth and invasion of colon cancer cells through inhibition of the hypoxia-inducible factor-2α pathway in vitro and in vivo. Mol Med Rep. 10:2401–2408. 2014.PubMed/NCBI
3	Su CC: Tanshinone IIA potentiates the efficacy of 5-FU in Colo205 colon cancer cells in vivo through downregulation of P-gp and LC3-II. Exp Ther Med. 3:555–559. 2012.PubMed/NCBI
4	Grijelmo C, Rodrigue C, Svrcek M, Bruyneel E, Hendrix A, de Wever O and Gespach C: Proinvasive activity of BMP-7 through SMAD4/src-independent and ERK/Rac/JNK-dependent signaling pathways in colon cancer cells. Cell Signal. 19:1722–1732. 2007. View Article : Google Scholar : PubMed/NCBI
5	Miskolci V, Rollins J, Vu HY, Ghosh CC, Davidson D and Vancurova I: NFkappaB is persistently activated in continuously stimulated human neutrophils. Mol Med. 13:134–142. 2007. View Article : Google Scholar : PubMed/NCBI
6	Luo W, Liu Y, Zhang J, Luo X, Lin C and Guo J: Andrographolide inhibits the activation of NF-κB and MMP-9 activity in H3255 lung cancer cells. Exp Ther Med. 6:743–746. 2013.PubMed/NCBI
7	Chelvarajan RL, Liu Y, Popa D, Getchell ML, Getchell TV, Stromberg AJ and Bondada S: Molecular basis of age-associated cytokine dysregulation in LPS-stimulated macrophages. J Leukoc Biol. 79:1314–1327. 2006. View Article : Google Scholar : PubMed/NCBI
8	Nakanishi C and Toi M: Nuclear factor-kappaB inhibitors as sensitizers to anticancer drugs. Nat Rev Cancer. 5:297–309. 2005. View Article : Google Scholar : PubMed/NCBI
9	Yu M, Tong X, Qi B, Qu H, Dong S, Yu B, Zhang N, Tang N, Wang L and Zhang C: Berberine enhances chemosensitivity to irinotecan in colon cancer via inhibition of NF-κB. Mol Med Rep. 9:249–254. 2014.PubMed/NCBI
10	Tanwar L, Vaish V and Sanyal SN: Chemoprevention of 1,2-dimethylhydrazine-induced colon carcinogenesis by a non-steroidal anti-inflammatory drug, etoricoxib, in rats: Inhibition of nuclear factor kappaB. Asian Pac J Cancer Prev. 10:1141–1146. 2009.PubMed/NCBI
11	Sato T, Hanada M, Bodrug S, Irie S, Iwama N, Boise LH, Thompson CB, Golemis E, Fong L, Wang HG, et al: Interactions among members of the Bcl-2 protein family analyzed with a yeast two-hybrid system. Proc Natl Acad Sci USA. 91:9238–9242. 1994. View Article : Google Scholar : PubMed/NCBI
12	Zhao L, Yu N, Guo T, Hou Y, Zeng Z, Yang X, Hu P, Tang X, Wang J and Liu M: Tissue biomarkers for prognosis of prostate cancer: A systematic review and meta-analysis. Cancer Epidemiol Biomarkers Prev. 23:1047–1054. 2014. View Article : Google Scholar : PubMed/NCBI
13	Giatromanolaki A, Stathopoulos GP, Koukourakis MI, Rigatos S, Vrettou E, Kittas C, Fountzilas G and Sivridis E: Angiogenesis and apoptosis-related protein (p53, bcl-2, and bax) expression versus response of gastric adenocarcinomas to paclitaxel and carboplatin chemotherapy. Am J Clin Oncol. 24:222–226. 2001. View Article : Google Scholar : PubMed/NCBI
14	Mirjolet JF, Barberi-Heyob M, Didelot C, Peyrat JP, Abecassis J, Millon R and Merlin JL: Bcl-2/Bax protein ratio predicts 5-fluorouracil sensitivity independently of p53 status. Br J Cancer. 83:1380–1386. 2000. View Article : Google Scholar : PubMed/NCBI
15	Ko YJ, Jeong JW, Choi YH and Ryu CH: Soy soluble polysaccharide induces apoptosis in HCT-116 human colon cancer cells via reactive oxygen species generation. Mol Med Rep. 8:1767–1772. 2013.PubMed/NCBI
16	Mao JD, Wu P, Xia XH, Hu JQ, Huang WB and Xu GQ: Correlation between expression of gastrin, somatostatin and cell apoptosis regulation gene bcl-2/bax in large intestine carcinoma. World J Gastroenterol. 11:721–725. 2005. View Article : Google Scholar : PubMed/NCBI
17	Yang HJ, Youn H, Seong KM, Yun YJ, Kim W, Kim YH, Lee JY, Kim CS, Jin YW and Youn B: Psoralidin, a dual inhibitor of COX-2 and 5-LOX, regulates ionizing radiation (IR)-induced pulmonary inflammation. Biochem Pharmacol. 82:524–534. 2011. View Article : Google Scholar : PubMed/NCBI
18	Hao W, Zhang X, Zhao W and Chen X: Psoralidin induces autophagy through ROS generation which inhibits the proliferation of human lung cancer A549 cells. PeerJ. 2:e5552014. View Article : Google Scholar : PubMed/NCBI
19	Pu Z, Zhang X, Chen Q, Yuan X and Xie H: Establishment of an expression platform of OATP1B1 388GG and 521CC genetic polymorphism and the therapeutic effect of tamoxifen in MCF-7 cells. Oncol Rep. 33:2420–2428. 2015.PubMed/NCBI
20	Wang S, Liu K, Seneviratne CJ, Li X, Cheung GS, Jin L, Chu CH and Zhang C: Lipoteichoic acid from an Enterococcus faecalis clinical strain promotes TNF-α expression through the NF-κB and p38 MAPK signaling pathways in differentiated THP-1 macrophages. Biomed Rep. 3:697–702. 2015.PubMed/NCBI
21	Han SA, Lee WY, Park CM, Yun SH and Chun HK: Comparison of immunologic outcomes of laparoscopic vs open approaches in clinical stage III colorectal cancer. Int J Colorectal Dis. 25:631–638. 2010. View Article : Google Scholar : PubMed/NCBI
22	Zhang L, Cai Q, Lin J, Fang Y, Zhan Y, Shen A, Wei L, Wang L and Peng J: Chloroform fraction of Scutellaria barbata D. Don promotes apoptosis and suppresses proliferation in human colon cancer cells. Mol Med Rep. 9:701–706. 2014.PubMed/NCBI
23	Touil Y, Igoudjil W, Corvaisier M, Dessein AF, Vandomme J, Monté D, Stechly L, Skrypek N, Langlois C, Grard G, et al: Colon cancer cells escape 5FU chemotherapy-induced cell death by entering stemness and quiescence associated with the c-Yes/YAP axis. Clin Cancer Res. 20:837–846. 2014. View Article : Google Scholar : PubMed/NCBI
24	Lo AC, Soliman AS, Khaled HM, Aboelyazid A and Greenson JK: Lifestyle, occupational, and reproductive factors and risk of colorectal cancer. Dis Colon Rectum. 53:830–837. 2010. View Article : Google Scholar : PubMed/NCBI
25	Szliszka E, Czuba ZP, Sędek Ł, Paradysz A and Król W: Enhanced TRAIL-mediated apoptosis in prostate cancer cells by the bioactive compounds neobavaisoflavone and psoralidin isolated from Psoralea corylifolia. Pharmacol Rep. 63:139–148. 2011. View Article : Google Scholar : PubMed/NCBI
26	Das TP, Suman S and Damodaran C: Induction of reactive oxygen species generation inhibits epithelial-mesenchymal transition and promotes growth arrest in prostate cancer cells. Mol Carcinog. 53:537–547. 2014. View Article : Google Scholar : PubMed/NCBI
27	Cohen M, Meisser A, Haenggeli L and Bischof P: Involvement of MAPK pathway in TNF-alpha-induced MMP-9 expression in human trophoblastic cells. Mol Hum Reprod. 12:225–232. 2006. View Article : Google Scholar : PubMed/NCBI
28	Davoudi Z, Akbarzadeh A, Rahmatiyamchi M, Movassaghpour AA, Alipour M, Nejati-Koshki K, Sadeghi Z, Dariushnejad H and Zarghami N: Molecular target therapy of AKT and NF-kB signaling pathways and multidrug resistance by specific cell penetrating inhibitor peptides in HL-60 cells. Asian Pac J Cancer Prev. 15:4353–4358. 2014. View Article : Google Scholar : PubMed/NCBI
29	Ali S, El-Rayes BF, Sarkar FH and Philip PA: Simultaneous targeting of the epidermal growth factor receptor and cyclooxygenase-2 pathways for pancreatic cancer therapy. Mol Cancer Ther. 4:1943–1951. 2005. View Article : Google Scholar : PubMed/NCBI
30	Chiou WF, Don MJ, Liao JF and Wei BL: Psoralidin inhibits LPS-induced iNOS expression via repressing Syk-mediated activation of PI3K-IKK-IκB signaling pathways. Eur J Pharmacol. 650:102–109. 2011. View Article : Google Scholar : PubMed/NCBI
31	Edwards MJ: Apoptosis, the heat shock response, hyperthermia, birth defects, disease and cancer. Where are the common links? Cell Stress Chaperones. 3:213–220. 1998. View Article : Google Scholar : PubMed/NCBI
32	Xiong Y, Ma XY, Zhang Z, Shao ZJ, Zhang YY and Zhou LM: Apoptosis induced by β,β-dimethylacrylshikonin is associated with Bcl-2 and NF-κB in human breast carcinoma MCF-7 cells. Oncol Lett. 6:1789–1793. 2013.PubMed/NCBI
33	Gu Y, Liu SL, Ju WZ, Li CY and Cao P: Analgesic-antitumor peptide induces apoptosis and inhibits the proliferation of SW480 human colon cancer cells. Oncol Lett. 5:483–488. 2013.PubMed/NCBI
34	Nam H and Kim MM: Ursolic acid induces apoptosis of SW480 cells via p53 activation. Food Chem Toxicol. 62:579–583. 2013. View Article : Google Scholar : PubMed/NCBI
35	Srinivasan S, Kumar R, Koduru S, Chandramouli A and Damodaran C: Inhibiting TNF-mediated signaling: A novel therapeutic paradigm for androgen independent prostate cancer. Apoptosis. 15:153–161. 2010. View Article : Google Scholar : PubMed/NCBI