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Article Open Access

MicroRNA-183-5p promotes the proliferation, invasion and metastasis of human pancreatic adenocarcinoma cells

Corrigendum in: /10.3892/ol.2020.12373
  • Authors:
    • Fei Miao
    • Jinhai Zhu
    • Yanlin Chen
    • Nanhong Tang
    • Xiaoqian Wang
    • Xiujin Li
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, Fujian Medical University Union Hospital, Fuzhou, Fujian 350001, P.R. China, Fujian Institute of Hepatobiliary Surgery, Fujian Medical University Union Hospital, Fuzhou, Fujian 350001, P.R. China
    Copyright: © Miao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 134-140
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    Published online on: November 5, 2015
       https://doi.org/10.3892/ol.2015.3872
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Abstract

The aim of the current study was to investigate the potential role of microRNA-183-5p (miR-183-5p) in the proliferation, invasion and metastasis of pancreatic cancer, and to identify promising target genes of oncogenic miR‑183‑5p. Western blotting and quantitative polymerase chain reaction (qPCR) were used to investigate whether these oncogenic microRNAs may be useful as biomarkers in pancreatic carcinoma (PaCa). Potential target genes were verified using miRDB, PicTar and TargetSCAN, and qPCR was used to detect the expression of miR‑183 and suppressor of cytokine signaling 6 (SOCS‑6; a potential target of miR-183) in PANC‑1 PaCa cells and in the HPDE6‑C7 pancreatic ductal cell line for comparison. The function of miR‑183 in cell proliferation, wound healing, invasion and migration was also investigated using a miR‑183 inhibitor. Western blot analysis was used to confirm SOCS‑6 as a tumor suppressor and qPCR was used to detect and confirm that this potential target gene is directly regulated by miR‑183. The results indicated that the expression of miR‑183 in PANC‑1 cells was upregulated compared with that in HPDE6‑C7 cells, whilst the expression of SOCS‑6 was downregulated. SOCS‑6 expression was also significantly lower in PaCa tissues compared with that in matched normal pancreatic tissues from PaCa patients. Furthermore, expression of miR‑183 was inversely correlated with that of SOCS‑6. miR‑183 knockdown decreased cell growth and motility in pancreatic cancer cells and significantly increased the expression of SOCS‑6. These data suggest that oncogenic miR‑183 may be useful as a pancreatic cancer biomarker. In addition, inhibition of miR‑183 expression may be beneficial as PaCa treatment. SOCS‑6 is a potential target gene of miR-183.
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Copy and paste a formatted citation
Spandidos Publications style
Miao F, Zhu J, Chen Y, Tang N, Wang X and Li X: MicroRNA-183-5p promotes the proliferation, invasion and metastasis of human pancreatic adenocarcinoma cells Corrigendum in /10.3892/ol.2020.12373. Oncol Lett 11: 134-140, 2016.
APA
Miao, F., Zhu, J., Chen, Y., Tang, N., Wang, X., & Li, X. (2016). MicroRNA-183-5p promotes the proliferation, invasion and metastasis of human pancreatic adenocarcinoma cells Corrigendum in /10.3892/ol.2020.12373. Oncology Letters, 11, 134-140. https://doi.org/10.3892/ol.2015.3872
MLA
Miao, F., Zhu, J., Chen, Y., Tang, N., Wang, X., Li, X."MicroRNA-183-5p promotes the proliferation, invasion and metastasis of human pancreatic adenocarcinoma cells Corrigendum in /10.3892/ol.2020.12373". Oncology Letters 11.1 (2016): 134-140.
Chicago
Miao, F., Zhu, J., Chen, Y., Tang, N., Wang, X., Li, X."MicroRNA-183-5p promotes the proliferation, invasion and metastasis of human pancreatic adenocarcinoma cells Corrigendum in /10.3892/ol.2020.12373". Oncology Letters 11, no. 1 (2016): 134-140. https://doi.org/10.3892/ol.2015.3872
Copy and paste a formatted citation
x
Spandidos Publications style
Miao F, Zhu J, Chen Y, Tang N, Wang X and Li X: MicroRNA-183-5p promotes the proliferation, invasion and metastasis of human pancreatic adenocarcinoma cells Corrigendum in /10.3892/ol.2020.12373. Oncol Lett 11: 134-140, 2016.
APA
Miao, F., Zhu, J., Chen, Y., Tang, N., Wang, X., & Li, X. (2016). MicroRNA-183-5p promotes the proliferation, invasion and metastasis of human pancreatic adenocarcinoma cells Corrigendum in /10.3892/ol.2020.12373. Oncology Letters, 11, 134-140. https://doi.org/10.3892/ol.2015.3872
MLA
Miao, F., Zhu, J., Chen, Y., Tang, N., Wang, X., Li, X."MicroRNA-183-5p promotes the proliferation, invasion and metastasis of human pancreatic adenocarcinoma cells Corrigendum in /10.3892/ol.2020.12373". Oncology Letters 11.1 (2016): 134-140.
Chicago
Miao, F., Zhu, J., Chen, Y., Tang, N., Wang, X., Li, X."MicroRNA-183-5p promotes the proliferation, invasion and metastasis of human pancreatic adenocarcinoma cells Corrigendum in /10.3892/ol.2020.12373". Oncology Letters 11, no. 1 (2016): 134-140. https://doi.org/10.3892/ol.2015.3872
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