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Article

Helicobacter pylori-infected MSCs acquire a pro-inflammatory phenotype and induce human gastric cancer migration by promoting EMT in gastric cancer cells

  • Authors:
    • Qiang Zhang
    • Juan Ding
    • Jinjun Liu
    • Wei Wang
    • Feng Zhang
    • Junhe Wang
    • Yuyun Li
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory Science, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, P.R. China, Department of Clinical Laboratory, Bengbu Medical College, Bengbu, Anhui 233030, P.R. China
  • Pages: 449-457
    |
    Published online on: November 10, 2015
       https://doi.org/10.3892/ol.2015.3897
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Abstract

Accumulating clinical and experimental evidence has suggested that Helicobacter pylori (H. pylori) infection‑associated gastric cancer (GC) is associated with high rates of mortality and serious health effects. The majority of patients succumb to H. pylori infection‑associated GC due to metastasis. Mesenchymal stem cells (MSCs), which have multipotent differentiation potential, may be recruited into the tumor‑associated stroma. MSCs are crucial components of the H. pylori infection‑associated GC microenvironment, and may be critical for GC cell migration. In this study, an MSCs/H. pylori co‑culture model was designed, and the effect of H. pylori‑infected MSCs on the migration of GC cells was evaluated using a Transwell migration assay. H. pylori‑infected MSC cytokine expression was evaluated using Luminex/ELISA. The expression of epithelial‑mesenchymal transition (EMT) markers in the GC cells treated with supernatants from H. pylori‑infected MSCs were detected by western blot analysis. The results demonstrated that the interaction between MSCs and H. pylori may induce GC cell migration, through secretion of a combination of cytokines that promote EMT in GC cells. The expression of phosphorylated forms of nuclear factor-κB (NF-κB) was observed to be increased in MSCs by H. pylori. Inhibition of NF‑κB activation by pyrrolidine dithiocarbamate blocked the effects of H. pylori‑infected MSCs on SGC‑7901 human stomach adenocarcinoma cell migration. Overall, the results of the present study suggest that H. pylori‑infected MSCs acquire a pro‑inflammatory phenotype through secretion of a combination of multiple cytokines, a number of which are NF‑κB‑dependent. These cytokines enhance H. pylori infection-associated GC cell migration by promoting EMT in GC cells. The results of the present study provide novel evidence for the modulatory effect of MSCs in the tumor microenvironment and provide insight into the significance of stromal cell involvement in GC progression.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Q, Ding J, Liu J, Wang W, Zhang F, Wang J and Li Y: Helicobacter pylori-infected MSCs acquire a pro-inflammatory phenotype and induce human gastric cancer migration by promoting EMT in gastric cancer cells. Oncol Lett 11: 449-457, 2016.
APA
Zhang, Q., Ding, J., Liu, J., Wang, W., Zhang, F., Wang, J., & Li, Y. (2016). Helicobacter pylori-infected MSCs acquire a pro-inflammatory phenotype and induce human gastric cancer migration by promoting EMT in gastric cancer cells. Oncology Letters, 11, 449-457. https://doi.org/10.3892/ol.2015.3897
MLA
Zhang, Q., Ding, J., Liu, J., Wang, W., Zhang, F., Wang, J., Li, Y."Helicobacter pylori-infected MSCs acquire a pro-inflammatory phenotype and induce human gastric cancer migration by promoting EMT in gastric cancer cells". Oncology Letters 11.1 (2016): 449-457.
Chicago
Zhang, Q., Ding, J., Liu, J., Wang, W., Zhang, F., Wang, J., Li, Y."Helicobacter pylori-infected MSCs acquire a pro-inflammatory phenotype and induce human gastric cancer migration by promoting EMT in gastric cancer cells". Oncology Letters 11, no. 1 (2016): 449-457. https://doi.org/10.3892/ol.2015.3897
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Q, Ding J, Liu J, Wang W, Zhang F, Wang J and Li Y: Helicobacter pylori-infected MSCs acquire a pro-inflammatory phenotype and induce human gastric cancer migration by promoting EMT in gastric cancer cells. Oncol Lett 11: 449-457, 2016.
APA
Zhang, Q., Ding, J., Liu, J., Wang, W., Zhang, F., Wang, J., & Li, Y. (2016). Helicobacter pylori-infected MSCs acquire a pro-inflammatory phenotype and induce human gastric cancer migration by promoting EMT in gastric cancer cells. Oncology Letters, 11, 449-457. https://doi.org/10.3892/ol.2015.3897
MLA
Zhang, Q., Ding, J., Liu, J., Wang, W., Zhang, F., Wang, J., Li, Y."Helicobacter pylori-infected MSCs acquire a pro-inflammatory phenotype and induce human gastric cancer migration by promoting EMT in gastric cancer cells". Oncology Letters 11.1 (2016): 449-457.
Chicago
Zhang, Q., Ding, J., Liu, J., Wang, W., Zhang, F., Wang, J., Li, Y."Helicobacter pylori-infected MSCs acquire a pro-inflammatory phenotype and induce human gastric cancer migration by promoting EMT in gastric cancer cells". Oncology Letters 11, no. 1 (2016): 449-457. https://doi.org/10.3892/ol.2015.3897
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