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Article Open Access

Phosphorylation of BMK1 induces prostatic carcinoma cell proliferation by promoting entry into the S phase of the cell cycle

  • Authors:
    • Ying Xiong
    • Luying Zhang
    • Tao Wang
  • View Affiliations / Copyright

    Affiliations: Department of Urology, First Affiliated Hospital of Yangtze University, Jingzhou, Hubei 434000, P.R. China, Division of Anatomy, Hubei College of Chinese Medicine, Jingzhou, Hubei 434100, P.R. China
    Copyright: © Xiong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 99-104
    |
    Published online on: November 10, 2015
       https://doi.org/10.3892/ol.2015.3909
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Abstract

Big mitogen-activated protein kinase 1 (BMK1) is activated by mitogens and oncogenic signals, and is strongly implicated in tumorigenesis. In the present study, it was demonstrated that the activation of BMK1, but not extracellular signal-regulated kinase (ERK)1/2, can induce proliferation in prostate cancer cells. It was found that the proliferation of epidermal growth factor (EGF)‑treated cells was accelerated by 40% compared with non‑treated cells using a CCK8 assay. In addition, cell cycle analysis using flow cytometry showed that the proportion of cells in the S phase increased significantly in the BMK1‑activated PC‑3 cells, suggesting that the activation of BMK1 promotes entry into the S phase of the cell cycle in prostate cancer cells. Furthermore, western blot analysis indicated that EGF‑mediated activation of BMK1, but not ERK1/2, participates in the proliferation and cell cycle regulation in prostate cancer cells. Furthermore, the effects of cell cycle regulation by the activation of BMK1 were associated with the increased expression levels of cyclin A and cyclin E, whereas the expression of cyclin B and cyclin D1 was unchanged in this process. Therefore, the present study demonstrated that the activation of BMK1 can induce proliferation by promoting entry into the S phase through the upregulation of cyclin A and cyclin E expression levels in prostate cancer cells.
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Copy and paste a formatted citation
Spandidos Publications style
Xiong Y, Zhang L and Wang T: Phosphorylation of BMK1 induces prostatic carcinoma cell proliferation by promoting entry into the S phase of the cell cycle. Oncol Lett 11: 99-104, 2016.
APA
Xiong, Y., Zhang, L., & Wang, T. (2016). Phosphorylation of BMK1 induces prostatic carcinoma cell proliferation by promoting entry into the S phase of the cell cycle. Oncology Letters, 11, 99-104. https://doi.org/10.3892/ol.2015.3909
MLA
Xiong, Y., Zhang, L., Wang, T."Phosphorylation of BMK1 induces prostatic carcinoma cell proliferation by promoting entry into the S phase of the cell cycle". Oncology Letters 11.1 (2016): 99-104.
Chicago
Xiong, Y., Zhang, L., Wang, T."Phosphorylation of BMK1 induces prostatic carcinoma cell proliferation by promoting entry into the S phase of the cell cycle". Oncology Letters 11, no. 1 (2016): 99-104. https://doi.org/10.3892/ol.2015.3909
Copy and paste a formatted citation
x
Spandidos Publications style
Xiong Y, Zhang L and Wang T: Phosphorylation of BMK1 induces prostatic carcinoma cell proliferation by promoting entry into the S phase of the cell cycle. Oncol Lett 11: 99-104, 2016.
APA
Xiong, Y., Zhang, L., & Wang, T. (2016). Phosphorylation of BMK1 induces prostatic carcinoma cell proliferation by promoting entry into the S phase of the cell cycle. Oncology Letters, 11, 99-104. https://doi.org/10.3892/ol.2015.3909
MLA
Xiong, Y., Zhang, L., Wang, T."Phosphorylation of BMK1 induces prostatic carcinoma cell proliferation by promoting entry into the S phase of the cell cycle". Oncology Letters 11.1 (2016): 99-104.
Chicago
Xiong, Y., Zhang, L., Wang, T."Phosphorylation of BMK1 induces prostatic carcinoma cell proliferation by promoting entry into the S phase of the cell cycle". Oncology Letters 11, no. 1 (2016): 99-104. https://doi.org/10.3892/ol.2015.3909
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