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Article

Myeloid‑derived suppressor cells enhance the expression of melanoma‑associated antigen A4 in a Lewis lung cancer murine model

  • Authors:
    • Guilan Shi
    • Huiru Wang
    • Xiufen Zhuang
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    Affiliations: Department of Immunology, Zibo Vocational Institute, Zibo, Shandong 255314, P.R. China, Department of Immunology, Cancer Institute, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, P.R. China, Department of Oncology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212000, P.R. China
  • Pages: 809-816
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    Published online on: November 13, 2015
       https://doi.org/10.3892/ol.2015.3918
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Abstract

The cancer‑testis (CT) family of antigens are expressed in multiple types of malignant neoplasm and are silent in normal tissues, apart from the testis. Immunotherapy targeting CT antigens is a promising therapeutic strategy for treatment of solid tumors. One member of this family, melanoma‑associated antigen A4 (MAGE‑A4), has been demonstrated to be expressed in melanomas and lung cancer. Patients with tumors expressing the MAGE‑A4 antigen exhibit specific cellular and humoral immune responses to the antigen, resulting in a favorable prognosis. Conversely, the expression of MAGE‑A4 is associated with poor survival in lung cancer. Myeloid‑derived suppressor cells (MDSCs) are a heterogeneous population of immunosuppressive cells, which are upregulated in the cancer microenvironment. Little is known regarding any potential correlation between the expression of MAGE‑A4 antigens and the accumulation of MDSCs. The present study aimed to examine the association between circulating MDSC levels and MAGE‑A4 expression in a mouse model of Lewis lung cancer. The expression of MAGE‑A4 in tumor cells or tissues was evaluated using western blotting, while the percentage of MDSCs (CD11b+Gr‑1+) in the blood was detected by flow cytometry. In addition, the suppressive capacity of MDSCs and the effectiveness of MDSC depletion were assessed in C57BL/6 tumor‑bearing mice. MDSCs were demonstrated to upregulate MAGE‑A4 expression via the phosphosphorylated‑signal transducer and activator of transcription 3705 pathway, while depletion of MDSCs decreased the tumor growth rate, prolonged median survival and enhanced the recognition of MAGE‑A4 by CD8+ T cells. These findings indicated that immunotherapeutic strategies involving induction of cytotoxic T lymphocytes that target MAGE‑A4, in combination with MDSC depletion, may be an effective approach to immunotherapy for cancer types with high expression of MAGE‑A4.
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Copy and paste a formatted citation
Spandidos Publications style
Shi G, Wang H and Zhuang X: Myeloid‑derived suppressor cells enhance the expression of melanoma‑associated antigen A4 in a Lewis lung cancer murine model. Oncol Lett 11: 809-816, 2016.
APA
Shi, G., Wang, H., & Zhuang, X. (2016). Myeloid‑derived suppressor cells enhance the expression of melanoma‑associated antigen A4 in a Lewis lung cancer murine model. Oncology Letters, 11, 809-816. https://doi.org/10.3892/ol.2015.3918
MLA
Shi, G., Wang, H., Zhuang, X."Myeloid‑derived suppressor cells enhance the expression of melanoma‑associated antigen A4 in a Lewis lung cancer murine model". Oncology Letters 11.1 (2016): 809-816.
Chicago
Shi, G., Wang, H., Zhuang, X."Myeloid‑derived suppressor cells enhance the expression of melanoma‑associated antigen A4 in a Lewis lung cancer murine model". Oncology Letters 11, no. 1 (2016): 809-816. https://doi.org/10.3892/ol.2015.3918
Copy and paste a formatted citation
x
Spandidos Publications style
Shi G, Wang H and Zhuang X: Myeloid‑derived suppressor cells enhance the expression of melanoma‑associated antigen A4 in a Lewis lung cancer murine model. Oncol Lett 11: 809-816, 2016.
APA
Shi, G., Wang, H., & Zhuang, X. (2016). Myeloid‑derived suppressor cells enhance the expression of melanoma‑associated antigen A4 in a Lewis lung cancer murine model. Oncology Letters, 11, 809-816. https://doi.org/10.3892/ol.2015.3918
MLA
Shi, G., Wang, H., Zhuang, X."Myeloid‑derived suppressor cells enhance the expression of melanoma‑associated antigen A4 in a Lewis lung cancer murine model". Oncology Letters 11.1 (2016): 809-816.
Chicago
Shi, G., Wang, H., Zhuang, X."Myeloid‑derived suppressor cells enhance the expression of melanoma‑associated antigen A4 in a Lewis lung cancer murine model". Oncology Letters 11, no. 1 (2016): 809-816. https://doi.org/10.3892/ol.2015.3918
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