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Article

Mangiferin inhibition of proliferation and induction of apoptosis in human prostate cancer cells is correlated with downregulation of B-cell lymphoma-2 and upregulation of microRNA-182

  • Authors:
    • Minglin Li
    • Huili Ma
    • Lixin Yang
    • Peng Li
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Nanyang City Center Hospital, Nanyang, Henan 473009, P.R. China
  • Pages: 817-822
    |
    Published online on: November 16, 2015
       https://doi.org/10.3892/ol.2015.3924
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Abstract

Mangiferin, a flavonoid extracted from the mango tree, possesses anti-inflammatory, antibacterial, anti-herpes simplex and antitumor activity, and is able to affect immune function. The present study investigated the anticancer effects of mangiferin treatment on PC3 human prostate cancer cells, and the potential underlying mechanisms. In the present study, an MTT assay was used to analyze the proliferation of PC3 cells. Subsequently, flow cytometry and colorimetric assay kits were utilized to measure the PC3 cell apoptotic rate. The expression levels of B‑cell lymphoma‑2 (Bcl‑2) and microRNA‑182 (miR‑182) were detected using western blot analysis and quantitative reverse transcription‑polymerase chain reaction, respectively. Finally, miR‑182 and anti‑miR‑182 were transfected into PC3 cells, which were used to investigate the effects of mangiferin. Mangiferin treatment reduced the proliferation of PC3 human prostate cancer cells in a concentration‑ and time‑dependent manner. In addition, mangiferin was able to promote apoptosis and induce the caspase‑3 activity of PC3 human prostate cancer cells. Mangiferin treatment was also able to significantly reduce Bcl‑2 expression levels and enhance miR‑182 expression in PC3 cells. Finally, it was observed that mangiferin inhibited proliferation and induced apoptosis in PC3 human prostate cancer cells, and this effect was correlated with downregulation of Bcl-2 and upregulation of miR-182.
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Copy and paste a formatted citation
Spandidos Publications style
Li M, Ma H, Yang L and Li P: Mangiferin inhibition of proliferation and induction of apoptosis in human prostate cancer cells is correlated with downregulation of B-cell lymphoma-2 and upregulation of microRNA-182. Oncol Lett 11: 817-822, 2016.
APA
Li, M., Ma, H., Yang, L., & Li, P. (2016). Mangiferin inhibition of proliferation and induction of apoptosis in human prostate cancer cells is correlated with downregulation of B-cell lymphoma-2 and upregulation of microRNA-182. Oncology Letters, 11, 817-822. https://doi.org/10.3892/ol.2015.3924
MLA
Li, M., Ma, H., Yang, L., Li, P."Mangiferin inhibition of proliferation and induction of apoptosis in human prostate cancer cells is correlated with downregulation of B-cell lymphoma-2 and upregulation of microRNA-182". Oncology Letters 11.1 (2016): 817-822.
Chicago
Li, M., Ma, H., Yang, L., Li, P."Mangiferin inhibition of proliferation and induction of apoptosis in human prostate cancer cells is correlated with downregulation of B-cell lymphoma-2 and upregulation of microRNA-182". Oncology Letters 11, no. 1 (2016): 817-822. https://doi.org/10.3892/ol.2015.3924
Copy and paste a formatted citation
x
Spandidos Publications style
Li M, Ma H, Yang L and Li P: Mangiferin inhibition of proliferation and induction of apoptosis in human prostate cancer cells is correlated with downregulation of B-cell lymphoma-2 and upregulation of microRNA-182. Oncol Lett 11: 817-822, 2016.
APA
Li, M., Ma, H., Yang, L., & Li, P. (2016). Mangiferin inhibition of proliferation and induction of apoptosis in human prostate cancer cells is correlated with downregulation of B-cell lymphoma-2 and upregulation of microRNA-182. Oncology Letters, 11, 817-822. https://doi.org/10.3892/ol.2015.3924
MLA
Li, M., Ma, H., Yang, L., Li, P."Mangiferin inhibition of proliferation and induction of apoptosis in human prostate cancer cells is correlated with downregulation of B-cell lymphoma-2 and upregulation of microRNA-182". Oncology Letters 11.1 (2016): 817-822.
Chicago
Li, M., Ma, H., Yang, L., Li, P."Mangiferin inhibition of proliferation and induction of apoptosis in human prostate cancer cells is correlated with downregulation of B-cell lymphoma-2 and upregulation of microRNA-182". Oncology Letters 11, no. 1 (2016): 817-822. https://doi.org/10.3892/ol.2015.3924
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