Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
January-2016 Volume 11 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2016 Volume 11 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

miR-128 regulates the apoptosis and proliferation of glioma cells by targeting RhoE

  • Authors:
    • Chao Shang
    • Yang Hong
    • Yan Guo
    • Yun‑Hui Liu
    • Yi‑Xue Xue
  • View Affiliations / Copyright

    Affiliations: Department of Neurobiology, College of Basic Medicine, China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Neurosurgery, Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, P.R. China, Department of Central Laboratory, School of Stomatology, China Medical University, Shenyang, Liaoning 110007, P.R. China
  • Pages: 904-908
    |
    Published online on: November 17, 2015
       https://doi.org/10.3892/ol.2015.3927
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

In this study, we investigate whether miR-128 is capable of regulating the apoptosis and proliferation of human U251 glioma cells by downregulating RhoE. The expression of miR‑128 was assessed by quantitative polymerase chain reaction in normal brain tissue and glioma samples. A significant downregulation of the expression of miR‑128 was detected in glioma in contrast to normal brain tissue. Following the transfection of pre‑miR‑128 and anti‑miR‑128 into U251 cells, the high expression of miR‑128 could inhibit proliferation and induce apoptosis in U251 cells, and those effects could be restored by miR‑128 knockdown. To analyze the regulation mechanism of miR‑128, TargetScan, miRanda and PicTar were used to ascertain whether RhoE was a potential target gene. Next, luciferase activity assay and western blot analysis confirmed that RhoE was a direct and specific target gene of miR‑128. The advanced effects of pre‑miR‑128 on the apoptosis and proliferation of U251 cells were reversed by the upregulation of RhoE expression. In summary, aberrantly expressed miR‑128 regulates apoptosis and proliferation in human glioma U251 cells partly by directly targeting RhoE. This finding may offer a new potential therapeutic strategy for the treatment of glioma.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Kuhnt D, Becker A, Ganslandt O, Bauer M, Buchfelder M and Nimsky C: Correlation of the extent of tumor volume resection and patient survival in surgery of glioblastoma multiforme with high-field intraoperative MRI guidance. Neuro Oncol. 13:1339–1348. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Mrugala MM: Advances and challenges in the treatment of glioblastoma: a clinician's perspective. Discov Med. 15:221–230. 2013.PubMed/NCBI

3 

Nieder C, Astner ST, Mehta MP, Grosu AL and Molls M: Improvement, clinical course, and quality of life after palliative radiotherapy for recurrent glioblastoma. Am J Clin Oncol. 31:300–305. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Shang C, Lu YM and Meng LR: MicroRNA-125b down-regulation mediates endometrial cancer invasion by targeting ERBB2. Med Sci Monit. 18:BR149–BR155. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Zhang H, Guo Y, Shang C, Song Y and Wu B: MiR-21 downregulated TCF21 to inhibit KISS1 in renal cancer. Urology. 80:1298–1302. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Hu J, Cheng Y, Li Y, Jin Z, Pan Y, Liu G, Fu S, Zhang Y, Feng K and Feng Y: MicroRNA-128 plays a critical role in human non-small cell lung cancer tumourigenesis, angiogenesis and lymphangiogenesis by directly targeting vascular endothelial growth factor-C. Eur J Cancer. 50:2336–2350. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Lin L, Chen X, Peng X, Zhou J, Kung HF, Lin MC and Jiang S: microRNA-128 promotes cell-cell adhesion in U87 glioma cells via regulation of EphB2. Oncol Rep. 30:1239–1248. 2013.PubMed/NCBI

8 

Shi ZM, Wang J, Yan Z, You YP, Li CY, Qian X, Yin Y, Zhao P, Wang YY, Wang XF, et al: MiR-128 inhibits tumor growth and angiogenesis by targeting p70S6K1. PLoS One. 7:e327092012. View Article : Google Scholar : PubMed/NCBI

9 

Adlakha YK and Saini N: MicroRNA-128 downregulates Bax and induces apoptosis in human embryonic kidney cells. Cell Mol Life Sci. 68:1415–1428. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Li M, Fu W, Wo L, Shu X, Liu F and Li C: MiR-128 and its target genes in tumorigenesis and metastasis. Exp Cell Res. 319:3059–3064. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Shang C, Zhang H, Guo Y, Hong Y, Liu Y and Xue Y: MiR-320a down-regulation mediates bladder carcinoma invasion by targeting ITGB3. Mol Biol Rep. 41:2521–2527. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Kumar M, Singh M and Singh SB: Optimization of conditions for expression of recombinant interferon-γ in E. coli. Mol Biol Rep. 41:6537–6543. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Dahlrot RH: The prognostic value of clinical factors and cancer stem cell-related markers in gliomas. Dan Med J. 61:B49442014.PubMed/NCBI

14 

Lim YC, Roberts TL, Day BW, Stringer BW, Kozlov S, Fazry S, Bruce ZC, Ensbey KS, Walker DG, Boyd AW and Lavin MF: Increased sensitivity to ionizing radiation by targeting the homologous recombination pathway in glioma initiating cells. Mol Oncol. 8:1603–1615. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Fukai J, Koizumi F and Nakao N: Enhanced anti-tumor effect of zoledronic acid combined with temozolomide against human malignant glioma cell expressing O6-methylguanine DNA methyltransferase. PLoS One. 9:e1045382014. View Article : Google Scholar : PubMed/NCBI

16 

Wang F, Zamora G, Sun CH, Trinidad A, Chun C, Kwon YJ, Berg K, Madsen SJ and Hirschberg H: Increased sensitivity of glioma cells to 5-fluorocytosine following photo-chemical internalization enhanced nonviral transfection of the cytosine deaminase suicide gene. J Neurooncol. 118:29–37. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Chen T, Wang XY, Li C and Xu SJ: Downregulation of microRNA-124 predicts poor prognosis in glioma patients. Neurol Sci. 36:131–135. 2015. View Article : Google Scholar : PubMed/NCBI

18 

Wang L, Shi ZM, Jiang CF, Liu X, Chen QD, Qian X, Li DM, Ge X, Wang XF, Liu LZ, et al: MiR-143 acts as a tumor suppressor by targeting N-RAS and enhances temozolomide-inducedapoptosis in glioma. Oncotarget. 5:5416–27. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Liu Y, Zhao J, Zhang PY, Zhang Y, Sun SY, Yu SY and Xi QS: MicroRNA-10b targets E-cadherin and modulates breast cancer metastasis. Med Sci Monit. 18:BR299–BR308. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Lonjedo M, Poch E, Mocholí E, Hernández-Sánchez M, Ivorra C, Franke TF, Guasch RM and Pérez-Roger I: The Rho family member RhoE interacts with Skp2 and is degraded at the proteasome during cell cycle progression. J Biol Chem. 288:30872–30882. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Tang Y, Hu C, Yang H, Cao L, Li Y, Deng P and Huang L: Rnd3 regulates lung cancer cell proliferation through notch signaling. PLoS One. 9:e1118972014. View Article : Google Scholar : PubMed/NCBI

22 

Riento K, Guasch RM, Garg R, Jin B and Ridley AJ: RhoE binds to ROCK I and inhibits downstream signaling. Mol Cell Biol. 23:4219–4229. 2003. View Article : Google Scholar : PubMed/NCBI

23 

Ma W, Wong CC, Tung EK, Wong CM and Ng IO: RhoE is frequently down-regulated in hepatocellular carcinoma (HCC) and suppresses HCC invasion through antagonizing the Rho/Rho-kinase/myosin phosphatase target pathway. Hepatology. 57:152–161. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Poch E, Miñambres R, Mocholí E, Ivorra C, Pérez-Aragó A, Guerri C, Pérez-Roger I and Guasch RM: RhoE interferes with Rb inactivation and regulates the proliferation and survival of the U87 human glioblastoma cell line. Exp Cell Res. 313:719–731. 2007. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Shang C, Hong Y, Guo Y, Liu YH and Xue YX: miR-128 regulates the apoptosis and proliferation of glioma cells by targeting RhoE. Oncol Lett 11: 904-908, 2016.
APA
Shang, C., Hong, Y., Guo, Y., Liu, Y., & Xue, Y. (2016). miR-128 regulates the apoptosis and proliferation of glioma cells by targeting RhoE. Oncology Letters, 11, 904-908. https://doi.org/10.3892/ol.2015.3927
MLA
Shang, C., Hong, Y., Guo, Y., Liu, Y., Xue, Y."miR-128 regulates the apoptosis and proliferation of glioma cells by targeting RhoE". Oncology Letters 11.1 (2016): 904-908.
Chicago
Shang, C., Hong, Y., Guo, Y., Liu, Y., Xue, Y."miR-128 regulates the apoptosis and proliferation of glioma cells by targeting RhoE". Oncology Letters 11, no. 1 (2016): 904-908. https://doi.org/10.3892/ol.2015.3927
Copy and paste a formatted citation
x
Spandidos Publications style
Shang C, Hong Y, Guo Y, Liu YH and Xue YX: miR-128 regulates the apoptosis and proliferation of glioma cells by targeting RhoE. Oncol Lett 11: 904-908, 2016.
APA
Shang, C., Hong, Y., Guo, Y., Liu, Y., & Xue, Y. (2016). miR-128 regulates the apoptosis and proliferation of glioma cells by targeting RhoE. Oncology Letters, 11, 904-908. https://doi.org/10.3892/ol.2015.3927
MLA
Shang, C., Hong, Y., Guo, Y., Liu, Y., Xue, Y."miR-128 regulates the apoptosis and proliferation of glioma cells by targeting RhoE". Oncology Letters 11.1 (2016): 904-908.
Chicago
Shang, C., Hong, Y., Guo, Y., Liu, Y., Xue, Y."miR-128 regulates the apoptosis and proliferation of glioma cells by targeting RhoE". Oncology Letters 11, no. 1 (2016): 904-908. https://doi.org/10.3892/ol.2015.3927
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team