miR-128 regulates the apoptosis and proliferation of glioma cells by targeting RhoE

Shang,Chao; Hong,Yang; Guo,Yan; Liu,Yun‑Hui; Xue,Yi‑Xue

doi:10.3892/ol.2015.3927

miR-128 regulates the apoptosis and proliferation of glioma cells by targeting RhoE

Authors:
- Chao Shang
- Yang Hong
- Yan Guo
- Yun‑Hui Liu
- Yi‑Xue Xue
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Affiliations: Department of Neurobiology, College of Basic Medicine, China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Neurosurgery, Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, P.R. China, Department of Central Laboratory, School of Stomatology, China Medical University, Shenyang, Liaoning 110007, P.R. China
Published online on: November 17, 2015 https://doi.org/10.3892/ol.2015.3927
Pages: 904-908

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Abstract

In this study, we investigate whether miR-128 is capable of regulating the apoptosis and proliferation of human U251 glioma cells by downregulating RhoE. The expression of miR‑128 was assessed by quantitative polymerase chain reaction in normal brain tissue and glioma samples. A significant downregulation of the expression of miR‑128 was detected in glioma in contrast to normal brain tissue. Following the transfection of pre‑miR‑128 and anti‑miR‑128 into U251 cells, the high expression of miR‑128 could inhibit proliferation and induce apoptosis in U251 cells, and those effects could be restored by miR‑128 knockdown. To analyze the regulation mechanism of miR‑128, TargetScan, miRanda and PicTar were used to ascertain whether RhoE was a potential target gene. Next, luciferase activity assay and western blot analysis confirmed that RhoE was a direct and specific target gene of miR‑128. The advanced effects of pre‑miR‑128 on the apoptosis and proliferation of U251 cells were reversed by the upregulation of RhoE expression. In summary, aberrantly expressed miR‑128 regulates apoptosis and proliferation in human glioma U251 cells partly by directly targeting RhoE. This finding may offer a new potential therapeutic strategy for the treatment of glioma.

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