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Genetic heterogeneity of RPMI-8402, a T-acute lymphoblastic leukemia cell line

  • Authors:
    • Ewelina Stoczynska‑Fidelus
    • Sylwester Piaskowski
    • Roza Pawlowska
    • Malgorzata Szybka
    • Joanna Peciak
    • Krystyna Hulas‑Bigoszewska
    • Marta Winiecka‑Klimek
    • Piotr Rieske
  • View Affiliations / Copyright

    Affiliations: Department of Tumor Biology, Medical University of Łódź, Łódź 90‑752, Poland, Department of Research and Development, Celther Polska Ltd., Łódź 93‑193, Poland, Department of Microbiology and Medical Laboratory Immunology, Medical University of Łódź, Łódź 92‑213, Poland
    Copyright: © Stoczynska‑Fidelus et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 593-599
    |
    Published online on: November 23, 2015
       https://doi.org/10.3892/ol.2015.3959
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Abstract

Thorough examination of genetic heterogeneity of cell lines is uncommon. In order to address this issue, the present study analyzed the genetic heterogeneity of RPMI-8402, a T-acute lymphoblastic leukemia (T-ALL) cell line. For this purpose, traditional techniques such as fluorescence in situ hybridization and immunocytochemistry were used, in addition to more advanced techniques, including cell sorting, Sanger sequencing and massive parallel sequencing. The results indicated that the RPMI‑8402 cell line consists of several genetically different cell subpopulations. Furthermore, massive parallel sequencing of RPMI‑8402 provided insight into the evolution of T‑ALL carcinogenesis, since this cell line exhibited the genetic heterogeneity typical of T‑ALL. Therefore, the use of cell lines for drug testing in future studies may aid the progress of anticancer drug research.
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1 

Gillet JP, Calcagno AM, Varma S, Marino M, Green LJ, Vora MI, Patel C, Orina JN, Eliseeva TA, Singal V, et al: Redefining the relevance of established cancer cell lines to the study of mechanisms of clinical anti-cancer drug resistance. Proc Natl Acad Sci USA. 108:18708–18713. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Gazdar AF, Gao B and Minna JD: Lung cancer cell lines: Useless artifacts or invaluable tools for medical science? Lung Cancer. 68:309–318. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Nugoli M, Chuchana P, Vendrell J, Orsetti B, Ursule L, Nguyen C, Birnbaum D, Douzery EJ, Cohen P and Theillet C: Genetic variability in MCF-7 sublines: Evidence of rapid genomic and RNA expression profile modifications. BMC Cancer. 3:132003. View Article : Google Scholar : PubMed/NCBI

4 

Inoue A, Yokomori K, Tanabe H, Mizusawa H, Sofuni T, Hayashi Y, Tsuchida Y and Shimatake H: Extensive genetic heterogeneity in the neuroblastoma cell line NB(TU)1. Int J Cancer. 72:1070–1077. 1997. View Article : Google Scholar : PubMed/NCBI

5 

Treanor LM, Zhou S, Janke L, Churchman ML, Ma Z, Lu T, Chen SC, Mullighan CG and Sorrentino BP: Interleukin-7 receptor mutants initiate early T cell precursor leukemia in murine thymocyte progenitors with multipotent potential. J Exp Med. 211:701–713. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Hait WN: Anticancer drug development: The grand challenges. Nat Rev Drug Discov. 9:253–254. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Hutchinson L and Kirk R: High drug attrition rates - where are we going wrong? Nat Rev Clin Oncol. 8:189–190. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Robinson JT, Thorvaldsdóttir H, Winckler W, Guttman M, Lander ES, Getz G and Mesirov JP: Integrative genomics viewer. Nat Biotechnol. 29:24–26. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Srivastava BI, Minowada J and Moore GE: High terminal deoxynucleotidyl transferase activity in a new T-cell line (RPMI 8402) of acute lymphoblastic leukemia origin. J Natl Cancer Inst. 55:11–14. 1975.PubMed/NCBI

10 

Gu Y, Lu K, Yang G, Cen Z, Yu L, Lin L, Hao J, Yang Z, Peng J, Cui S and Huang J: Mutation spectrum of six genes in Chinese phenylketonuria patients obtained through next-generation sequencing. PLoS One. 9:e941002014. View Article : Google Scholar : PubMed/NCBI

11 

Cai X, Sheng J, Tang C, Nandakumar V, Ye H, Ji H, Tang H, Qin Y, Guan H, Lou F, et al: Frequent mutations in EGFR, KRAS and TP53 genes in human lung cancer tumors detected by ion torrent DNA sequencing. PLoS One. 9:e952282014. View Article : Google Scholar : PubMed/NCBI

12 

Bai X, Zhang E, Ye H, Nandakumar V, Wang Z, Chen L, Tang C, Li J, Li H, Zhang W, et al: PIK3CA and TP53 gene mutations in human breast cancer tumors frequently detected by ion torrent DNA sequencing. PLoS One. 9:e993062014. View Article : Google Scholar : PubMed/NCBI

13 

Mitra A, Mishra L and Li S: Technologies for deriving primary tumor cells for use in personalized cancer therapy. Trends Biotechnol. 31:347–354. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Marjanovic ND, Weinberg RA and Chaffer CL: Cell plasticity and heterogeneity in cancer. Clin Chem. 59:168–179. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Vogelstein B and Kinzler KW: Cancer genes and the pathways they control. Nat Med. 10:789–799. 2004. View Article : Google Scholar : PubMed/NCBI

16 

Gebhart E, Thoma K, Verdorfer I, Drexler HG and Efferth T: Genomic imbalances in T-cell acute lymphoblastic leukemia cell lines. Int J Oncol. 21:887–894. 2002.PubMed/NCBI

17 

Gerlinger M, Horswell S, Larkin J, Rowan AJ, Salm MP, Varela I, Fisher R, McGranahan N, Matthews N, Santos CR, et al: Genomic architecture and evolution of clear cell renal cell carcinomas defined by multiregion sequencing. Nat Genet. 46:225–233. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Sottoriva A, Spiteri I, Piccirillo SG, Touloumis A, Collins VP, Marioni JC, Curtis C, Watts C and Tavaré S: Intratumor heterogeneity in human glioblastoma reflects cancer evolutionary dynamics. Proc Natl Acad Sci USA. 110:4009–4014. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Mullighan CG: The molecular genetic makeup of acute lymphoblastic leukemia. Hematology Am Soc Hematol Educ Program. 2012:389–396. 2012.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Stoczynska‑Fidelus E, Piaskowski S, Pawlowska R, Szybka M, Peciak J, Hulas‑Bigoszewska K, Winiecka‑Klimek M and Rieske P: Genetic heterogeneity of RPMI-8402, a T-acute lymphoblastic leukemia cell line. Oncol Lett 11: 593-599, 2016.
APA
Stoczynska‑Fidelus, E., Piaskowski, S., Pawlowska, R., Szybka, M., Peciak, J., Hulas‑Bigoszewska, K. ... Rieske, P. (2016). Genetic heterogeneity of RPMI-8402, a T-acute lymphoblastic leukemia cell line. Oncology Letters, 11, 593-599. https://doi.org/10.3892/ol.2015.3959
MLA
Stoczynska‑Fidelus, E., Piaskowski, S., Pawlowska, R., Szybka, M., Peciak, J., Hulas‑Bigoszewska, K., Winiecka‑Klimek, M., Rieske, P."Genetic heterogeneity of RPMI-8402, a T-acute lymphoblastic leukemia cell line". Oncology Letters 11.1 (2016): 593-599.
Chicago
Stoczynska‑Fidelus, E., Piaskowski, S., Pawlowska, R., Szybka, M., Peciak, J., Hulas‑Bigoszewska, K., Winiecka‑Klimek, M., Rieske, P."Genetic heterogeneity of RPMI-8402, a T-acute lymphoblastic leukemia cell line". Oncology Letters 11, no. 1 (2016): 593-599. https://doi.org/10.3892/ol.2015.3959
Copy and paste a formatted citation
x
Spandidos Publications style
Stoczynska‑Fidelus E, Piaskowski S, Pawlowska R, Szybka M, Peciak J, Hulas‑Bigoszewska K, Winiecka‑Klimek M and Rieske P: Genetic heterogeneity of RPMI-8402, a T-acute lymphoblastic leukemia cell line. Oncol Lett 11: 593-599, 2016.
APA
Stoczynska‑Fidelus, E., Piaskowski, S., Pawlowska, R., Szybka, M., Peciak, J., Hulas‑Bigoszewska, K. ... Rieske, P. (2016). Genetic heterogeneity of RPMI-8402, a T-acute lymphoblastic leukemia cell line. Oncology Letters, 11, 593-599. https://doi.org/10.3892/ol.2015.3959
MLA
Stoczynska‑Fidelus, E., Piaskowski, S., Pawlowska, R., Szybka, M., Peciak, J., Hulas‑Bigoszewska, K., Winiecka‑Klimek, M., Rieske, P."Genetic heterogeneity of RPMI-8402, a T-acute lymphoblastic leukemia cell line". Oncology Letters 11.1 (2016): 593-599.
Chicago
Stoczynska‑Fidelus, E., Piaskowski, S., Pawlowska, R., Szybka, M., Peciak, J., Hulas‑Bigoszewska, K., Winiecka‑Klimek, M., Rieske, P."Genetic heterogeneity of RPMI-8402, a T-acute lymphoblastic leukemia cell line". Oncology Letters 11, no. 1 (2016): 593-599. https://doi.org/10.3892/ol.2015.3959
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