Novel method for the detection and quantification of malignant cells in the CSF of patients with leptomeningeal metastasis of lung cancer

Ma,Chunhua; Lv,Yuan; Jiang,Rong; Li,Jinduo; Wang,Bin; Sun,Liwei

doi:10.3892/ol.2015.3971

January-2016 Volume 11 Issue 1

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January-2016 Volume 11 Issue 1

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Article Open Access

Novel method for the detection and quantification of malignant cells in the CSF of patients with leptomeningeal metastasis of lung cancer

Authors:
- Chunhua Ma
- Yuan Lv
- Rong Jiang
- Jinduo Li
- Bin Wang
- Liwei Sun
View Affiliations / Copyright

Affiliations: Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Disease, Department of Intervention, Tianjin Huanhu Hospital, Tianjin 300060, P.R. China

Copyright: © Ma et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 619-623
|
Published online on: November 25, 2015

https://doi.org/10.3892/ol.2015.3971
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Abstract

The aim of the present study was to discuss a novel method for the detection of malignant tumor cells in cerebrospinal fluid (CSF), by observing tumor marker‑immunostaining fluorescence in situ hybridization (TM‑iFISH) enrichment and by counting CSF malignant tumor cells in patients with lung cancer leptomeningeal metastasis (LM). A total of 10 CSF samples were collected from 6 patients that presented with lung cancer LM. For each patient, 20 ml CSF was obtained through a lumbar puncture, of which 7.5 ml was used to count the number of malignant tumor cells in the CSF using TM‑iFISH enrichment. Cytological and biochemical examinations were conducted on the remaining 10 ml and 2.5 ml CSF, respectively. The 10 CSF samples were successfully analyzed by TM‑iFISH, and the tumor cell count range was 3‑1,823 cells/7.5 ml CSF in 7 of the samples detected. There were no tumor cells detected in the remaining 3 samples. Tumor cells were revealed in 3 of the samples through the CSF cytological examinations, and albumin protein levels were indicated to be greater than the normal range (normal range, 0.15-0.45 g/l), in 9 of the samples using CSF biochemical examinations. Additionally, TM‑iFISH was performed again to count the CSF malignant tumor cells in 3 of the patients following intrathecal injection of chemotherapy (methotrexate 10 mg and dexamethasone 5 mg). The results indicated that the malignant tumor cell count of 2 of the patients had decreased in comparison to the pre‑treatment cell count. As it is capable of enriching and counting CSF malignant tumor cells in patients with lung cancer LM, TM‑iFISH may be an effective method to diagnose lung cancer LM and to evaluate its efficacy.

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1	Leal T, Chang JE, Mehta M and Robins HI: Leptomeningeal metastasis: Challenges in diagnosis and treatment. Curr Cancer Ther Rev. 7:319–327. 2011. View Article : Google Scholar : PubMed/NCBI
2	Nagpal S, Riess J and Wakelee H: Treatment of leptomeningeal spread of NSCLC: A continuing challenge. Curr Treat Options Oncol. 13:491–504. 2012. View Article : Google Scholar : PubMed/NCBI
3	Liu ZH, Zhang DL and Wang L: The value of cerebrospinal fluid cytological checkup in diagnosing meningeal carcinomatosis. Zhonghua Quan Ke Yi Xue. 8:187–188. 2010.
4	Wang P, She CH, Li P, Pu YZ, Wang XG and Li WL: Significance of tumor markers in the diagnosis of lung cancer meningeal metastasis. Zhongguo Zhong Liu Lin Chuang. 35:61–64. 2008.
5	Le Rhun E, Massin F, Tu Q, Bonneterre J, De Carvalho Bittencourt M and Faure GC: Development of a new method for identification and quantification in cerebrospinal fluid of malignant cells from breast carcinoma leptomeningeal metastasis. BMC Clin Pathol. 12:212012. View Article : Google Scholar : PubMed/NCBI
6	Igawa S, Gohda K, Fukui T, Ryuge S, Otani S, Masago A, Sato J, Murakami K, Maki S, Katono K, et al: Circulating tumor cells as a prognostic factor in patients with small cell lung cancer. Oncol Lett. 7:1469–1473. 2014.PubMed/NCBI
7	Yu Y, Chen Z, Dong J, Wei P, Hu R, Zhou C, Sun N, Luo M, Yang W, Yao R, et al: Folate receptor-positive circulating tumor cells as a novel diagnostic biomarker in non-small cell lung cancer. Transl Oncol. 6:697–702. 2013. View Article : Google Scholar : PubMed/NCBI
8	Romiti A, Raffa S, Di Rocco R, Roberto M, Milano A, Zullo A, Leone L, Ranieri D, Mazzetta F, Medda E, et al: Circulating tumor cells count predicts survival in colorectal cancer patients. J Gastrointestin Liver Dis. 23:279–284. 2014.PubMed/NCBI
9	Jiang R, Ma CH, Zhu ZL, Li JD, Wang B, Sun LW and Lv Y: Application of circulating tumor cell detection in cerebrospinal fluid in the diagnosis of meningeal metastasis from non-small cell lung cancer. Zhong Guo Xiandai Shenjig Jibing Zazhi. 14:698–701. 2014.
10	Wang Y, Gao Y, Zhu YF and Tao RJ: The diagnosis and treatments progress of meningeal carcinomatosis. Zhongguo Lin Chuang Shen Jing Wai Ke Za Zhi. 18:760–762. 2013.
11	Ma C, Jiang R, Li J, Wang B, Sun L and Lv Y: Research progress of lung cancer with leptomeningeal metastasis. Zhongguo Fei Ai Za Zhi. 17:695–700. 2014.(In Chinese). PubMed/NCBI
12	Clarke JL, Perez HR, Jacks LM, Panageas KS and Deangelis LM: Leptomeningeal metastases in the MRI era. Neurology. 74:1449–1454. 2010. View Article : Google Scholar : PubMed/NCBI
13	Le Rhun E, Tu Q, De Carvalho Bittencourt M, Farre I, Mortier L, Cai H, Kohler C and Faure GC: Detection and quantification of CSF malignant cells by the CellSearch technology in patients with melanoma leptomeningeal metastasis. Med Oncol. 30:5382013. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Novel method for the detection and quantification of malignant cells in the CSF of patients with leptomeningeal metastasis of lung cancer

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Abstract

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