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Article

Apelin‑13 induces autophagy in hepatoma HepG2 cells through ERK1/2 signaling pathway‑dependent upregulation of Beclin1

Expression of Concern in: /10.3892/ol.2025.15236
  • Authors:
    • Qiulin Huang
    • Xuan Liu
    • Chao Cao
    • Junyue Lei
    • Dong Han
    • Guodong Chen
    • Jia Yu
    • Linxi Chen
    • Deguan Lv
    • Zhongyu Li
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, First Affiliated Hospital of University of South China, Hengyang, Hunan 421001, P.R. China, Institute of Pharmacy and Pharmacology, University of South China, Hengyang, Hunan 421001, P.R. China, Department of Microbiology, School of Medicine, University of South China, Hengyang, Hunan 421001, P.R. China
  • Pages: 1051-1056
    |
    Published online on: December 3, 2015
       https://doi.org/10.3892/ol.2015.3991
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Abstract

The aim of the present study was to investigate the effect of Apelin‑13 on autophagy in hepatocellular carcinoma HepG2 cells and the underlying mechanism of the effect. The HepG2 cells were treated with Apelin‑13 at a final concentration of 0.0001, 0.001, 0.01 and 0.1 µmol/l for 24 h. Cells were also treated with 10 µmol/l PD98059 for 24 h. The expression of the extracellular signal‑regulated kinase (ERK)1/2, phosphorylated ERK1/2 (pERK1/2) and Beclin1 proteins were detected by western blot analysis. Beclin1 mRNA expression was also detected by reverse transcription‑polymerase chain reaction. Autophagy was observed using fluorescence microscopy subsequent to monodansylcadaverine (MDC) staining. Following treatment with the various concentrations of Apelin‑13, the expression of the ERK1/2 protein remained at a similar level, whereas the expression of pERK1/2 increased in a dose‑dependent manner. Compared with the control group, the increase was significant (P<0.05). Similarly, Beclin1 expression was upregulated at the protein and mRNA levels by Apelin‑13 treatment in a dose‑dependent manner and was significantly increased compared with the control group. However, following treatment with the Apelin‑13 inhibitor PD98059, the expression of pERK1/2, Beclin1 protein and Beclin1 mRNA were significantly decreased (P<0.05). In addition, Apelin‑13 induced the autophagy of HepG2 cells in a dose‑dependent manner, as revealed by MDC staining. PD98059 inhibited autophagy of HepG2 cells induced by Apelin‑13. Therefore, Apelin‑13 may promote autophagy in HepG2 cells by inducing the phosphorylation of ERK1/2 and upregulating the expression of Beclin1.
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Copy and paste a formatted citation
Spandidos Publications style
Huang Q, Liu X, Cao C, Lei J, Han D, Chen G, Yu J, Chen L, Lv D, Li Z, Li Z, et al: Apelin‑13 induces autophagy in hepatoma HepG2 cells through ERK1/2 signaling pathway‑dependent upregulation of Beclin1 Expression of Concern in /10.3892/ol.2025.15236. Oncol Lett 11: 1051-1056, 2016.
APA
Huang, Q., Liu, X., Cao, C., Lei, J., Han, D., Chen, G. ... Li, Z. (2016). Apelin‑13 induces autophagy in hepatoma HepG2 cells through ERK1/2 signaling pathway‑dependent upregulation of Beclin1 Expression of Concern in /10.3892/ol.2025.15236. Oncology Letters, 11, 1051-1056. https://doi.org/10.3892/ol.2015.3991
MLA
Huang, Q., Liu, X., Cao, C., Lei, J., Han, D., Chen, G., Yu, J., Chen, L., Lv, D., Li, Z."Apelin‑13 induces autophagy in hepatoma HepG2 cells through ERK1/2 signaling pathway‑dependent upregulation of Beclin1 Expression of Concern in /10.3892/ol.2025.15236". Oncology Letters 11.2 (2016): 1051-1056.
Chicago
Huang, Q., Liu, X., Cao, C., Lei, J., Han, D., Chen, G., Yu, J., Chen, L., Lv, D., Li, Z."Apelin‑13 induces autophagy in hepatoma HepG2 cells through ERK1/2 signaling pathway‑dependent upregulation of Beclin1 Expression of Concern in /10.3892/ol.2025.15236". Oncology Letters 11, no. 2 (2016): 1051-1056. https://doi.org/10.3892/ol.2015.3991
Copy and paste a formatted citation
x
Spandidos Publications style
Huang Q, Liu X, Cao C, Lei J, Han D, Chen G, Yu J, Chen L, Lv D, Li Z, Li Z, et al: Apelin‑13 induces autophagy in hepatoma HepG2 cells through ERK1/2 signaling pathway‑dependent upregulation of Beclin1 Expression of Concern in /10.3892/ol.2025.15236. Oncol Lett 11: 1051-1056, 2016.
APA
Huang, Q., Liu, X., Cao, C., Lei, J., Han, D., Chen, G. ... Li, Z. (2016). Apelin‑13 induces autophagy in hepatoma HepG2 cells through ERK1/2 signaling pathway‑dependent upregulation of Beclin1 Expression of Concern in /10.3892/ol.2025.15236. Oncology Letters, 11, 1051-1056. https://doi.org/10.3892/ol.2015.3991
MLA
Huang, Q., Liu, X., Cao, C., Lei, J., Han, D., Chen, G., Yu, J., Chen, L., Lv, D., Li, Z."Apelin‑13 induces autophagy in hepatoma HepG2 cells through ERK1/2 signaling pathway‑dependent upregulation of Beclin1 Expression of Concern in /10.3892/ol.2025.15236". Oncology Letters 11.2 (2016): 1051-1056.
Chicago
Huang, Q., Liu, X., Cao, C., Lei, J., Han, D., Chen, G., Yu, J., Chen, L., Lv, D., Li, Z."Apelin‑13 induces autophagy in hepatoma HepG2 cells through ERK1/2 signaling pathway‑dependent upregulation of Beclin1 Expression of Concern in /10.3892/ol.2025.15236". Oncology Letters 11, no. 2 (2016): 1051-1056. https://doi.org/10.3892/ol.2015.3991
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