Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
February-2016 Volume 11 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
February-2016 Volume 11 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Effect of miR‑155 knockdown on the reversal of doxorubicin resistance in human lung cancer A549/dox cells

  • Authors:
    • Lixia Lv
    • Xiumei An
    • Hongyan Li
    • Lanxiu Ma
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, Tianjin Huanhu Hospital, Tianjin 300060, P.R. China, Biotechnological Immunology Laboratory, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, P.R. China
  • Pages: 1161-1166
    |
    Published online on: December 3, 2015
       https://doi.org/10.3892/ol.2015.3995
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Doxorubicin has been widely used in the treatment of cancer. However, acquired doxorubicin resistance severely hinders the application of the drug. In the present study, doxorubicin resistance was investigated in lung carcinoma. microRNA‑155 (miR‑155) was found to be upregulated in the doxorubicin‑resistant A549/dox cell line. Suppression of miR‑155 in this cell line considerably reversed doxorubicin resistance, and doxorubicin‑induced apoptosis and cell cycle arrest were recovered. Furthermore, reverse transcription‑polymerase chain reaction and western blot analysis revealed that miR‑155 suppression downregulated the expression of multidrug resistance protein 1, multidrug resistance‑associated protein 1, breast cancer resistance protein, glutathione S‑transferase‑π, Survivin and B‑cell lymphoma 2, and upregulated the expression of caspase‑3 and caspase‑8. In addition, it was found that miR‑155 suppression inhibited the activation of AKT and extracellular signal‑regulated kinase. The transcriptional activity of nuclear factor‑κB and activator protein‑1 was also downregulated. In summary, the present results indicate that miR‑155 may participate in doxorubicin resistance in lung carcinoma. The current study provides a novel target for lung carcinoma treatment.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Niu X, Liu S, Jia L and Chen J: Role of MiR-3619-5p in β-catenin-mediated non-small cell lung cancer growth and invasion. Cell Physiol Biochem. 4:1527–1536. 2015. View Article : Google Scholar

2 

Won YK, Lee JY, Kang YN, Jang JS, Kang JH, Jung SL, Sung SY, Jo IY, Park HH, Lee DS, et al: Stereotactic radiosurgery for brain metastasis in non-small cell lung cancer. Radiat Oncol J. 3:207–216. 2015. View Article : Google Scholar

3 

Hoffman PC, Mauer AM and Vokes EE: Lung cancer. Lancet. 355:479–485. 2000. View Article : Google Scholar : PubMed/NCBI

4 

Molina JR, Yang P, Cassivi SD, Schild SE and Adjei AA: Non-small cell lung cancer, Epidemiology, risk factors, treatment and survivorship. Mayo Clin Proc. 83:584–594. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Siegel R, Ma J, Zou Z and Jemal A: Cancer statistics, 2014. CA Cancer J Clin. 64:9–29. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Janku F, Stewart DJ and Kurzrock R: Targeted therapy in non-small-cell lung cancer-is it becoming a reality? Nat Rev Clin Oncol. 7:401–414. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Agarwal ML, Taylor WR, Chernov MV, Chernova OB and Stark GR: The p53 network. J Biol Chem. 273:1–4. 1998. View Article : Google Scholar : PubMed/NCBI

8 

Müller I, Niethammer D and Bruchelt G: Anthracycline-derived chemotherapeutics in apoptosis and free radical cytotoxicity (Review). Int J Mol Med. 1:491–494. 1998.PubMed/NCBI

9 

Zhu T, Li LL, Xiao GF, Luo QZ, Liu QZ, Yao KT and Xiao GH: Berberine increases doxorubicin sensitivity by suppressing STAT3 in lung cancer. Am J Chin Med. 27:1–16. 2015.

10 

Li ZL, Chen C, Yang Y, Wang C, Yang T, Yang X and Liu SC: Gamma secretase inhibitor enhances sensitivity to doxorubicin in MDA-MB-231 cells. Int J Clin Exp Pathol. 5:4378–4387. 2015.

11 

Calin GA and Croce CM: MicroRNA signatures in human cancers. Nat Rev Cancer. 6:857–866. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Carthew RW and Sontheimer EJ: Origins and Mechanisms of miRNAs and siRNAs. Cell. 136:642–655. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Valencia-Sanchez MA, Liu J, Hannon GJ and Parker R: Control of translation and mRNA degradation by miRNAs and siRNAs. Genes Dev. 20:515–524. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Yuan Y, Yao YF, Hu SN, Gao J and Zhang LL: MiR-133a is functionally involved in doxorubicin-resistance in breast cancer cells MCF-7 via its regulation of the expression of uncoupling protein 2. PLoS One. 6:e01298432015. View Article : Google Scholar

15 

Gandellini P, Profumo V, Folini M and Zaffaroni N: MicroRNAs as new therapeutic targets and tools in cancer. Expert Opin Ther Targets. 15:265–279. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Trang P, Weidhaas JB and Slack FJ: MicroRNAs as potential cancer therapeutics. Oncogene. 27((Suppl 2)): S52–S57. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Wang F, Zhou J, Zhang Y, Wang Y, Cheng L, Bai Y and Ma H: The value of microRNA-155 as a prognostic factor for survival in non-small cell lung cancer, A meta-analysis. PLoS One. 8:e01368892015. View Article : Google Scholar

18 

Sochor M, Basova P, Pesta M, Dusilkova N, Bartos J, Burda P, Pospisil V and Stopka T: Oncogenic microRNAs, MiR-155, miR-19a, miR-181b and miR-24 enable monitoring of early breast cancer in serum. BMC Cancer. 14:4482014. View Article : Google Scholar : PubMed/NCBI

19 

Yan Z, Che S, Wang J, Jiao Y, Wang C and Meng Q: miR-155 contributes to the progression of glioma by enhancing Wnt/β-catenin pathway. Tumour Biol. 7:5323–5331. 2015. View Article : Google Scholar

20 

Hongliang C, Shaojun H, Aihua L and Hua J: Correlation between expression of miR-155 in colon cancer and serum carcinoembryonic antigen level and its contribution to recurrence and metastasis forecast. Saudi Med J. 35:547–553. 2014.PubMed/NCBI

21 

Li T, Yang J, Lv X, Liu K, Gao C, Xing Y and Xi T: MiR-155 regulates the proliferation and cell cycle of colorectal carcinoma cells by targeting E2F2. Biotechnol Lett. 36:1743–1752. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Merhautova J, Hezova R, Poprach A, Kovarikova A, Radova L, Svoboda M, Vyzula R, Demlova R and Slaby O: miR-155 and miR-484 are associated with time to progression in metastatic renal cell carcinoma treated with sunitinib. Biomed Res Int. 2015:9419802015. View Article : Google Scholar : PubMed/NCBI

23 

Zhu Y, Liu XJ, Yang P, Zhao M, Lv LX, Zhang GD, Wang Q and Zhang L: Alkylglyceronephosphate synthase (AGPS) alters lipid signaling pathways and supports chemotherapy resistance of glioma and hepatic carcinoma cell lines. Asian Pac J Cancer Prev. 15:3219–3226. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Fruman DA and Rommel C: PI3K and cancer: Lessons challenges and opportunities. Nat Rev Drug Discov. 13:140–156. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Hendijani F: JavanmardS hH, Rafiee L and Sadeghi-Aliabadi H: Effect of human Wharton's jelly mesenchymal stem cell secretome on proliferation, apoptosis and drug resistance of lung cancer cells. Res Pharm Sci. 2:134–142. 2015.

26 

Zhao YY, Yu L, Liu BL, He XJ and Zhang BY: Downregulation of P-gp, Ras and p-ERK1/2 contributes to the arsenic trioxide-induced reduction in drug resistance towards doxorubicin in gastric cancer cell lines. Mol Med Rep. 5:7335–7343. 2015.

27 

Keppler D: Multidrug resistance proteins (MRPs, ABCC s): Importance for pathophysiology and drug therapy. Handb Exp Pharmacol. 299–323. 2011. View Article : Google Scholar : PubMed/NCBI

28 

Chen S, Jiao JW, Sun KX, Zong ZH and Zhao Y: MicroRNA-133b targets glutathione S-transferase π expression to increase ovarian cancer cell sensitivity to chemotherapy drugs. Drug Des Devel Ther. 9:5225–5535. 2015.PubMed/NCBI

29 

Surowiak P, Materna V, Kaplenko I, Spaczyński M, Dietel M, Lage H and Zabel M: Augmented expression of metallothionein and glutathione S-transferase pi as unfavourable prognostic factors in cisplatin-treated ovarian cancer patients. Virchows Arch. 447:626–633. 2005. View Article : Google Scholar : PubMed/NCBI

30 

Stewart DJ: Mechanisms of resistance to cisplatin and carboplatin. Crit Rev Oncol Hematol. 63:12–31. 2007. View Article : Google Scholar : PubMed/NCBI

31 

de Moraes Nestal G, Delbue D, Silva KL, Robaina MC, Khongkow P, Gomes AR, Zona S, Crocamo S, Mencalha AL, Magalhães LM, Lam EW and Maia RC: FOXM1 targets XIAP and Survivin to modulate breast cancer survival and chemoresistance. Cell Signal. 12:2496–2505. 2015. View Article : Google Scholar

32 

Ryan BM, O'Donovan N and Duffy MJ: Survivin A new target for anti-cancer therapy. Cancer Treat Rev. 35:553–562. 2009. View Article : Google Scholar : PubMed/NCBI

33 

Altieri DC: Survivin and IAP proteins in cell-death mechanisms. Biochem J. 430:199–205. 2010. View Article : Google Scholar : PubMed/NCBI

34 

Yip KW and Reed JC: Bcl-2 family proteins and cancer. Oncogene. 27:6398–6406. 2008. View Article : Google Scholar : PubMed/NCBI

35 

Ghavami S, Hashemi M, Ande SR, Yeganeh B, Xiao W, Eshraghi M, Bus CJ, Kadkhoda K, Wiechec E, Halayko AJ and Los M: Apoptosis and cancer, Mutations within caspase genes. J Med Genet. 46:497–510. 2009. View Article : Google Scholar : PubMed/NCBI

36 

Chakraborty C: GeorgeP riya Doss C and Bandyopadhyay S: miRNAs in insulin resistance and diabetes-associated pancreatic cancer: The 'minute and miracle' molecule moving as a monitor in the 'genomic galaxy'. Curr Drug Targets. 10:1110–1117. 2013. View Article : Google Scholar

37 

Zhang E and Wu Y: Dual effects of miR-155 on macrophages at different stages of atherosclerosis: LDL is the key? Med Hypotheses. 83:74–78. 2014. View Article : Google Scholar : PubMed/NCBI

38 

Nitiss JL: Targeting DNA topoisomerase II in cancer chemotherapy. Nat Rev Cancer. 9:338–350. 2009. View Article : Google Scholar : PubMed/NCBI

39 

Kong W, He L, Coppola M, Guo J, Esposito NN, Coppola D and Cheng JQ: MicroRNA-155 regulates cell survival, growth, and chemosensitivity by targeting FOXO3a in breast cancer. J Biol Chem. 23:17869–17879. 2010. View Article : Google Scholar

40 

Cai ZK, Chen Q, Chen YB, Gu M, Zheng DC, Zhou J and Wang Z: microRNA-155 promotes the proliferation of prostate cancer cells by targeting annexin 7. Mol Med Rep. 1:533–538. 2015.

41 

Xue P, Zheng M, Diao Z, Shen L, Liu M, Gong P, Sun H and Hu Y: miR-155* mediates suppressive effect of PTEN 3′-untranslated region on AP-1/NF-κB pathway in HTR-8/SVneo cells. Placenta. 8:650–656. 2013. View Article : Google Scholar

42 

Farooqi AA, Qureshi MZ, Coskunpinar E, Naqvi SK, Yaylim I and Ismail M: MiR-421, miR-155 and miR-650: Emerging trends of regulation of cancer and apoptosis. Asian Pac J Cancer Prev. 15:1909–1912. 2014. View Article : Google Scholar : PubMed/NCBI

43 

Gasparini P, Lovat F, Fassan M, Casadei L, Cascione L, Jacob NK, Carasi S, Palmieri D, Costinean S, Shapiro CL, et al: Protective role of miR-155 in breast cancer through RAD51 targeting impairs homologous recombination after irradiation. Proc Natl Acad Sci USA. 111:4536–4541. 2014. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Lv L, An X, Li H and Ma L: Effect of miR‑155 knockdown on the reversal of doxorubicin resistance in human lung cancer A549/dox cells. Oncol Lett 11: 1161-1166, 2016.
APA
Lv, L., An, X., Li, H., & Ma, L. (2016). Effect of miR‑155 knockdown on the reversal of doxorubicin resistance in human lung cancer A549/dox cells. Oncology Letters, 11, 1161-1166. https://doi.org/10.3892/ol.2015.3995
MLA
Lv, L., An, X., Li, H., Ma, L."Effect of miR‑155 knockdown on the reversal of doxorubicin resistance in human lung cancer A549/dox cells". Oncology Letters 11.2 (2016): 1161-1166.
Chicago
Lv, L., An, X., Li, H., Ma, L."Effect of miR‑155 knockdown on the reversal of doxorubicin resistance in human lung cancer A549/dox cells". Oncology Letters 11, no. 2 (2016): 1161-1166. https://doi.org/10.3892/ol.2015.3995
Copy and paste a formatted citation
x
Spandidos Publications style
Lv L, An X, Li H and Ma L: Effect of miR‑155 knockdown on the reversal of doxorubicin resistance in human lung cancer A549/dox cells. Oncol Lett 11: 1161-1166, 2016.
APA
Lv, L., An, X., Li, H., & Ma, L. (2016). Effect of miR‑155 knockdown on the reversal of doxorubicin resistance in human lung cancer A549/dox cells. Oncology Letters, 11, 1161-1166. https://doi.org/10.3892/ol.2015.3995
MLA
Lv, L., An, X., Li, H., Ma, L."Effect of miR‑155 knockdown on the reversal of doxorubicin resistance in human lung cancer A549/dox cells". Oncology Letters 11.2 (2016): 1161-1166.
Chicago
Lv, L., An, X., Li, H., Ma, L."Effect of miR‑155 knockdown on the reversal of doxorubicin resistance in human lung cancer A549/dox cells". Oncology Letters 11, no. 2 (2016): 1161-1166. https://doi.org/10.3892/ol.2015.3995
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team