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Article

Mesenchymal stem cells promote tumor angiogenesis via the action of transforming growth factor β1

  • Authors:
    • Guo‑Cai Li
    • Hong‑Wei Zhang
    • Qing‑Chun Zhao
    • Li Sun
    • Jian‑Jun Yang
    • Liu Hong
    • Fan Feng
    • Lei Cai
  • View Affiliations / Copyright

    Affiliations: Department of Digestive Surgery, Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China
  • Pages: 1089-1094
    |
    Published online on: December 3, 2015
       https://doi.org/10.3892/ol.2015.3997
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Abstract

Mesenchymal stem cells (MSCs) may influence the growth and metastasis of various human malignancies, including hepatocellular carcinoma (HCC). Therefore, the underlying mechanisms via which MSCs are able to affect malignancies require investigation. In the present study, the potential role of MSC in the angiogenesis of HCC was investigated. A total of 17 nude mouse models exhibiting human HCC were used to evaluate the effects of MSC on angiogenesis. A total of 8 mice were injected with human MSCs via the tail vein, and the remaining 9 mice were injected with phosphate‑buffered saline as a control. A total of 35 days subsequent to the injection of MSCs, the microvessel density (MVD) of tumors was evaluated by immunostaining, using cluster of differentiation 31 antibody. The mRNA levels of transforming growth factor (TGF)β1, Smad2 and Smad7 were detected using reverse transcription‑quantitative polymerase chain reaction. Protein expression levels of TGFβ1 and vascular endothelial growth factor (VEGF) in tumor tissues were analyzed using ELISA. Compared with controls, MVD in MSC‑treated mice was significantly increased (28.00±9.19 vs. 18.11±3.30; P=0.006). The levels of TGFβ1 mRNA in the MSC‑treated group were 2.15‑fold higher compared with the control group (1.27±0.61 vs. 0.59±0.39; P=0.033), and MVD was higher in the group exhibiting increased TGFβ1 mRNA levels compared with the control group (26.50±9.11 vs. 19.44±6.14; P=0.038). In addition, a close correlation between the expression levels of TGFβ1 and VEGF was identified. The results of the present study suggested that MSCs may be capable of enhancing the angiogenesis of HCC, which may be partly due to the involvement of TGFβ1.
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Copy and paste a formatted citation
Spandidos Publications style
Li GC, Zhang HW, Zhao QC, Sun L, Yang JJ, Hong L, Feng F and Cai L: Mesenchymal stem cells promote tumor angiogenesis via the action of transforming growth factor β1. Oncol Lett 11: 1089-1094, 2016.
APA
Li, G., Zhang, H., Zhao, Q., Sun, L., Yang, J., Hong, L. ... Cai, L. (2016). Mesenchymal stem cells promote tumor angiogenesis via the action of transforming growth factor β1. Oncology Letters, 11, 1089-1094. https://doi.org/10.3892/ol.2015.3997
MLA
Li, G., Zhang, H., Zhao, Q., Sun, L., Yang, J., Hong, L., Feng, F., Cai, L."Mesenchymal stem cells promote tumor angiogenesis via the action of transforming growth factor β1". Oncology Letters 11.2 (2016): 1089-1094.
Chicago
Li, G., Zhang, H., Zhao, Q., Sun, L., Yang, J., Hong, L., Feng, F., Cai, L."Mesenchymal stem cells promote tumor angiogenesis via the action of transforming growth factor β1". Oncology Letters 11, no. 2 (2016): 1089-1094. https://doi.org/10.3892/ol.2015.3997
Copy and paste a formatted citation
x
Spandidos Publications style
Li GC, Zhang HW, Zhao QC, Sun L, Yang JJ, Hong L, Feng F and Cai L: Mesenchymal stem cells promote tumor angiogenesis via the action of transforming growth factor β1. Oncol Lett 11: 1089-1094, 2016.
APA
Li, G., Zhang, H., Zhao, Q., Sun, L., Yang, J., Hong, L. ... Cai, L. (2016). Mesenchymal stem cells promote tumor angiogenesis via the action of transforming growth factor β1. Oncology Letters, 11, 1089-1094. https://doi.org/10.3892/ol.2015.3997
MLA
Li, G., Zhang, H., Zhao, Q., Sun, L., Yang, J., Hong, L., Feng, F., Cai, L."Mesenchymal stem cells promote tumor angiogenesis via the action of transforming growth factor β1". Oncology Letters 11.2 (2016): 1089-1094.
Chicago
Li, G., Zhang, H., Zhao, Q., Sun, L., Yang, J., Hong, L., Feng, F., Cai, L."Mesenchymal stem cells promote tumor angiogenesis via the action of transforming growth factor β1". Oncology Letters 11, no. 2 (2016): 1089-1094. https://doi.org/10.3892/ol.2015.3997
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