Novel cyclic pentapeptide H-15 induces differentiation and inhibits proliferation in murine melanoma B16 cells

Liu,Yunjiang; Zhang,Geng; Wang,Haoqi; Liu,Shouxin; Chen,Jinxia; Zhao,Lianmei; Li,Jie; Shan,Baoen

doi:10.3892/ol.2015.4025

February-2016 Volume 11 Issue 2

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

February-2016 Volume 11 Issue 2

Full Size Image

Article

Novel cyclic pentapeptide H-15 induces differentiation and inhibits proliferation in murine melanoma B16 cells

Authors:
- Yunjiang Liu
- Geng Zhang
- Haoqi Wang
- Shouxin Liu
- Jinxia Chen
- Lianmei Zhao
- Jie Li
- Baoen Shan
View Affiliations / Copyright

Affiliations: Breast Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China, Research Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China, State Key Laboratory Breeding Base‑Hebei Province Key Laboratory of Molecular Chemistry for Drug, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, P.R. China
Pages: 1251-1255
|
Published online on: December 10, 2015

https://doi.org/10.3892/ol.2015.4025
Expand metrics +

Abstract

Sansalvamide A is a cyclic depsipeptide that is isolated from a marine fungus of the Fusarium genus. Sansalvamide A exhibits significant antitumor ability. The molecular formula and molecular weight of the novel sansalvamide A derivative H‑15 are C29H44BrN5O6 and 637.2475, respectively. In the present study, H‑15 was found to inhibit the proliferation and induce the differentiation of murine melanoma B16 cells. A sulforhodamine B colorimetric assay was used to measure the inhibitory effects of 0.1, 1, 10, 50 and 100 µM H‑15 on the B16 cells, and the results revealed that the inhibitory effects of H‑15 exerted on the B16 cells occurred in a concentration‑dependent manner. In addition, the growth curve model of the B16 cells treated with 50 µM H‑15 revealed that the effect of H‑15 was also time‑dependent. The differentiation morphology of the B16 cells was observed subsequent to treating the cells with H‑15. An optical microscope was used to observe the differentiation morphology of the cells. In addition, melanin secretion increased in the B16 cells treated with 50 µM H‑15. The expression levels of tyrosinase (TYR) were assayed by western blot analysis, and it was found that the cells treated with 50 µM H‑15 for 48 h exhibited increased expression of TYR. The results of the present study indicated that H-15 may induce the differentiation of B16 cells.

View Figures

View References

1	Gray-Schopfer V, Wellbrock C and Marais R: Melanoma biology and new targeted therapy. Nature. 445:851–857. 2007. View Article : Google Scholar : PubMed/NCBI
2	Berk LB: Radiation therapy as primary and adjuvant treatment for local and regional melanoma. Cancer Control. 15:233–238. 2008.PubMed/NCBI
3	MacQuarrie KL, Yao Z, Fong AP, Diede SJ, Rudzinski ER, Hawkins DS and Tapscott SJ: Comparison of genome-wide binding of MyoD in normal human myogenic cells and rhabdomyosarcomas identifies regional and local suppression of promyogenic transcription factors. Mol Cell Biol. 33:773–784. 2013. View Article : Google Scholar : PubMed/NCBI
4	Manu D.T, Sarika M, Jadhav S and Jayesh RB: All-trans retinoic acid loaded block copolymer nanoparticles efficiently induce cellular differentiation in HL-60 cells. Eur J Pharm Sci. 44:643–652. 2011. View Article : Google Scholar : PubMed/NCBI
5	Belofsky GN, Jensen PR and Fenical W: Sansalvamide A new cytotoxic cyclic depsipeptide produced by a marine fungus of the genus Fusarium. Tetrahedron Lett. 40:2913–2916. 1999. View Article : Google Scholar
6	Liu S, Gu W, Lo D, Ding XZ, Ujiki M, Adrian TE, Soff GA and Silverman RB: N-methylSansalvamide A peptide analogues. Histopathology. J Med Chem. 48:3630–3638. 2005. View Article : Google Scholar : PubMed/NCBI
7	Heiferman MJ, Salabat MR, Ujiki MB, Strouch MJ, Cheon EC, Silverman RB and Bentrem DJ: Sansalvamide induces pancreatic cancer growth arrest through changes in the cell cycle. Anticancer Res. 30:73–78. 2010.PubMed/NCBI
8	Styers TJ, Kekec A, Rodriguez R, Brown JD, Cajica J, Pan PS, Parry E, Carroll CL, et al: Synthesis of Sansalvamide A derivatives and their cytotoxicity in the MSS colon cancer cell line HT-29. Bioorg Med Chem. 14:5625–5631. 2006. View Article : Google Scholar : PubMed/NCBI
9	Amidon GL and Lee HJ: Absorption of peptide and peptidomimetic drugs. Annu Rev Pharmacol Toxicol. 34:321–341. 1994. View Article : Google Scholar : PubMed/NCBI
10	Zhang G, Liu S, Liu YJ, Wang F, Ren J, Gu J, Zhou K and Shan B: A novel cyclic pentapeptide, H-10, inhibits B16 cancer cell growth and induces cell apoptosis. Oncology Lett. 8:248–252. 2014.
11	Serra JM, Gutiérrez A, Alemany R, Navarro M, Ros T, Saus C, Ginés J, Sampol A, Amat JC, Serra-Moisés L, et al: Inhibition of c-Myc down-regulation by sustained extracellular signal-regulated kinase activation prevents the antimetabolite methotrexate- and gemcitabine-induced differentiation in non-small-cell lung cancer cells. Mol Pharmacol. 73:1679–1687. 2008. View Article : Google Scholar : PubMed/NCBI
12	Leszczyniecka M, Roberts T, Dent P, Grant S and Fisher PB: Differentiation therapy of human cancer, Basic science and clinical applications. Pharmacol Ther. 90:105–156. 2001. View Article : Google Scholar : PubMed/NCBI
13	Jiang Z, Xu J, Long M, Tu Z, Yang G and He G: 2,3,5, 4′-tetrahydroxystilbene-2-O-beta-D-glucoside (THSG) induces melanogenesis in B16 cells by MAP kinase activation and tyrosinase upregulation. Life Sci. 85:345–350. 2009. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Novel cyclic pentapeptide H-15 induces differentiation and inhibits proliferation in murine melanoma B16 cells

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Related Articles