Picfeltarraenin IA inhibits lipopolysaccharide-induced inflammatory cytokine production by the nuclear factor-κB pathway in human pulmonary epithelial A549 cells

  • Authors:
    • Rong Shi
    • Qing Wang
    • Yang Ouyang
    • Qian Wang
    • Xudong Xiong
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  • Published online on: December 16, 2015     https://doi.org/10.3892/ol.2015.4037
  • Pages: 1195-1200
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Abstract

The present study aimed to investigate the effect of picfeltarraenin IA (IA) on respiratory inflammation by analyzing its effect on interleukin (IL)‑8 and prostaglandin E2 (PGE2) production. The expression of cyclooxygenase 2 (COX2) in human pulmonary adenocarcinoma epithelial A549 cells in culture was also examined. Human pulmonary epithelial A549 cells and the human monocytic leukemia THP‑1 cell line were used in the current study. Cell viability was measured using a methylthiazol tetrazolium assay. The production of IL‑8 and PGE2 was investigated using an enzyme‑linked immunosorbent assay. The expression of COX2 and nuclear factor‑κB (NF‑κB)‑p65 was examined using western blot analysis. Treatment with lipopolysaccharide (LPS; 10 µg/ml) resulted in the increased production of IL‑8 and PGE2, and the increased expression of COX2 in the A549 cells. Furthermore, IA (0.1‑10 µmol/l) significantly inhibited PGE2 production and COX2 expression in cells with LPS‑induced IL‑8, in a concentration‑dependent manner. The results suggested that IA downregulates LPS‑induced COX2 expression, and inhibits IL‑8 and PGE2 production in pulmonary epithelial cells. Additionally, IA was observed to suppress the expression of COX2 in THP‑1 cells, and also to regulate the expression of COX2 via the NF‑κB pathway in the A549 cells, but not in the THP‑1 cells. These results indicate that IA regulates LPS‑induced cytokine release in A549 cells via the NF-κB pathway.
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February-2016
Volume 11 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Shi R, Wang Q, Ouyang Y, Wang Q and Xiong X: Picfeltarraenin IA inhibits lipopolysaccharide-induced inflammatory cytokine production by the nuclear factor-κB pathway in human pulmonary epithelial A549 cells . Oncol Lett 11: 1195-1200, 2016.
APA
Shi, R., Wang, Q., Ouyang, Y., Wang, Q., & Xiong, X. (2016). Picfeltarraenin IA inhibits lipopolysaccharide-induced inflammatory cytokine production by the nuclear factor-κB pathway in human pulmonary epithelial A549 cells . Oncology Letters, 11, 1195-1200. https://doi.org/10.3892/ol.2015.4037
MLA
Shi, R., Wang, Q., Ouyang, Y., Wang, Q., Xiong, X."Picfeltarraenin IA inhibits lipopolysaccharide-induced inflammatory cytokine production by the nuclear factor-κB pathway in human pulmonary epithelial A549 cells ". Oncology Letters 11.2 (2016): 1195-1200.
Chicago
Shi, R., Wang, Q., Ouyang, Y., Wang, Q., Xiong, X."Picfeltarraenin IA inhibits lipopolysaccharide-induced inflammatory cytokine production by the nuclear factor-κB pathway in human pulmonary epithelial A549 cells ". Oncology Letters 11, no. 2 (2016): 1195-1200. https://doi.org/10.3892/ol.2015.4037