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Evaluation of the cytotoxic effects of Cyperus longus extract, fractions and its essential oil on the PC3 and MCF7 cancer cell lines

  • Authors:
    • Toktam Memariani
    • Toktam Hosseini
    • Hossein Kamali
    • Ameneh Mohammadi
    • Maryam Ghorbani
    • Abdoreza Shakeri
    • Demetrios A. Spandidos
    • Aristidis M. Tsatsakis
    • Shabnam Shahsavand
  • View Affiliations / Copyright

    Affiliations: Central Research Laboratory, North Khorasan University of Medical Sciences, Bojnurd, Iran, Department of General Health, School of Health Sciences, North Khorasan University of Medical Sciences, Bojnurd, Iran, Targeted Drug Delivery Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran, Research Center of Natural Products and Medicinal Plants, North Khorasan University of Medical Sciences, Bojnurd, Iran, Department of Pharmacology and Toxicology, School of Pharmacy, Baqiyatallah University of Medical Sciences, Tehran, Iran, Department of Pediatrics, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran, Laboratory of Clinical Virology, School of Medicine, University of Crete, Heraklion, Greece, Department of Forensic Sciences and Toxicology, School of Medicine, University of Crete, Heraklion, Greece
    Copyright: © Memariani et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1353-1360
    |
    Published online on: December 23, 2015
       https://doi.org/10.3892/ol.2015.4050
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Abstract

Cyperus longus is one of the Iranian endemic species. However, to date, and to the best of our knowledge, there are no availale academic reports on the cytotoxicity of this plant. Thus, this study was carried out to examine the in vitro anti‑proliferative and anti‑apoptotic effects of Cyperus longus extract, fractions and essential oil (EO) on MCF7 and PC3 cell lines. The chemical constituents of EO were identified using gas chromatography (GC)‑mass spectrometry (MS) analysis. The cells were cultured in RPMI‑1640 medium and incubated with various concentrations of the plant extract and fractions. Cell viability was quantified by MTT assay following 24, 48 and 72 h of exposure to (12.5‑200 µg/ml) of the methanol extract, the dichloromethane (CH2Cl2), ethyl acetate (EtOAc) and water fractions, as well as the EO of the plant. The percentage of apoptotic cells was determined using propidium iodide staining of DNA fragments by flow cytometry (sub‑G1 peak). The most effective fraction in the MCF7 cell line was the CH2Cl2 fraction (IC50 after 48 h, 25.34±2.01). The EtOAc fraction (IC50 after 48 h, 35.2±2.69) and the methanol extract (IC50 after 48 h, 64.64±1.64) were also found to be effective. The IC50 values obtained for the PC3 cell line were 37.97±3.87, 51.57±3.87 and 70.33±2.36 for the CH2Cl2 fraction, the EtOAc fraction and the methanol extract, respectively. Based on these data and due to the partial polarity of the most effective fraction (the CH2Cl2 fraction), we also examined the cytotoxicity of the plant EO. The IC50 values after 48 h were 22.25±4.25 and 12.55±3.65 in the PC3 and MCF7 cell lines, respectively. DNA fragmentation assay also confirmed these data. Performing GC‑MS analysis for the plant EO revealed that β‑himachalene (10.81%), α‑caryophyllene oxide (7.6%), irisone (4.78%), β‑caryophyllene oxide (4.36%), humulene oxide (12%), viridiflorol (4.73%), aristolone (6.39%) and longiverbenone (6.04%) were the main constituents. Our results demonstrated that two of the constituents of Cyperus longus, viridiflorol and longiverbenone, should be investigated further as possible promising chemotherapeutic agents in cancer treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Memariani T, Hosseini T, Kamali H, Mohammadi A, Ghorbani M, Shakeri A, Spandidos DA, Tsatsakis AM and Shahsavand S: Evaluation of the cytotoxic effects of Cyperus longus extract, fractions and its essential oil on the PC3 and MCF7 cancer cell lines. Oncol Lett 11: 1353-1360, 2016.
APA
Memariani, T., Hosseini, T., Kamali, H., Mohammadi, A., Ghorbani, M., Shakeri, A. ... Shahsavand, S. (2016). Evaluation of the cytotoxic effects of Cyperus longus extract, fractions and its essential oil on the PC3 and MCF7 cancer cell lines. Oncology Letters, 11, 1353-1360. https://doi.org/10.3892/ol.2015.4050
MLA
Memariani, T., Hosseini, T., Kamali, H., Mohammadi, A., Ghorbani, M., Shakeri, A., Spandidos, D. A., Tsatsakis, A. M., Shahsavand, S."Evaluation of the cytotoxic effects of Cyperus longus extract, fractions and its essential oil on the PC3 and MCF7 cancer cell lines". Oncology Letters 11.2 (2016): 1353-1360.
Chicago
Memariani, T., Hosseini, T., Kamali, H., Mohammadi, A., Ghorbani, M., Shakeri, A., Spandidos, D. A., Tsatsakis, A. M., Shahsavand, S."Evaluation of the cytotoxic effects of Cyperus longus extract, fractions and its essential oil on the PC3 and MCF7 cancer cell lines". Oncology Letters 11, no. 2 (2016): 1353-1360. https://doi.org/10.3892/ol.2015.4050
Copy and paste a formatted citation
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Spandidos Publications style
Memariani T, Hosseini T, Kamali H, Mohammadi A, Ghorbani M, Shakeri A, Spandidos DA, Tsatsakis AM and Shahsavand S: Evaluation of the cytotoxic effects of Cyperus longus extract, fractions and its essential oil on the PC3 and MCF7 cancer cell lines. Oncol Lett 11: 1353-1360, 2016.
APA
Memariani, T., Hosseini, T., Kamali, H., Mohammadi, A., Ghorbani, M., Shakeri, A. ... Shahsavand, S. (2016). Evaluation of the cytotoxic effects of Cyperus longus extract, fractions and its essential oil on the PC3 and MCF7 cancer cell lines. Oncology Letters, 11, 1353-1360. https://doi.org/10.3892/ol.2015.4050
MLA
Memariani, T., Hosseini, T., Kamali, H., Mohammadi, A., Ghorbani, M., Shakeri, A., Spandidos, D. A., Tsatsakis, A. M., Shahsavand, S."Evaluation of the cytotoxic effects of Cyperus longus extract, fractions and its essential oil on the PC3 and MCF7 cancer cell lines". Oncology Letters 11.2 (2016): 1353-1360.
Chicago
Memariani, T., Hosseini, T., Kamali, H., Mohammadi, A., Ghorbani, M., Shakeri, A., Spandidos, D. A., Tsatsakis, A. M., Shahsavand, S."Evaluation of the cytotoxic effects of Cyperus longus extract, fractions and its essential oil on the PC3 and MCF7 cancer cell lines". Oncology Letters 11, no. 2 (2016): 1353-1360. https://doi.org/10.3892/ol.2015.4050
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