MicroRNA‑26a induces osteosarcoma cell growth and metastasis via the Wnt/β‑catenin pathway

  • Authors:
    • Feng Qu
    • Chun‑Bao Li
    • Bang‑Tuo Yuan
    • Wei Qi
    • Hong‑Liang Li
    • Xue‑Zhen Shen
    • Gang Zhao
    • Jiang‑Tao Wang
    • Yu‑Jie Liu
  • View Affiliations

  • Published online on: December 31, 2015     https://doi.org/10.3892/ol.2015.4073
  • Pages: 1592-1596
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Abstract

MicroRNAs (miRNAs/miRs) are a type of highly conserved, small non‑coding RNA that are vital to the post‑transcriptional regulation of gene expression via base pairing with target mRNA 3'‑untranslated regions (3'‑UTRs). Several studies have indicated that the abnormal expression of miRNAs occurs frequently in human osteosarcoma (OS). In the present study, the role of miR‑26a in the progression and metastasis of OS was investigated using reverse transcription‑quantitative polymerase chain reaction, a luciferase activity assay, cell viability assay, in vitro migration and invasion assays, transfection and western blot analysis. miR‑26a was upregulated in OS tissues and cell lines, and the expression of miR‑26a was indicated to affect the proliferation, migration and invasion of OS Saos‑2 cells. At the molecular level, the results showed that glycogen synthase kinase‑3β (GSK‑3β) was identified as a target of miR‑26a, and the ectopic expression of miR‑26a inhibited GSK‑3β by directly binding to the 3'‑UTR. Therefore, the expression of miR‑26a was negatively correlated with GSK‑3β in the OS tissues. These data suggest that miR‑26a is significant in the proliferation of human OS cells due to the direct regulation of Wnt/β‑catenin signaling.
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February-2016
Volume 11 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Qu F, Li CB, Yuan BT, Qi W, Li HL, Shen XZ, Zhao G, Wang JT and Liu YJ: MicroRNA‑26a induces osteosarcoma cell growth and metastasis via the Wnt/β‑catenin pathway. Oncol Lett 11: 1592-1596, 2016
APA
Qu, F., Li, C., Yuan, B., Qi, W., Li, H., Shen, X. ... Liu, Y. (2016). MicroRNA‑26a induces osteosarcoma cell growth and metastasis via the Wnt/β‑catenin pathway. Oncology Letters, 11, 1592-1596. https://doi.org/10.3892/ol.2015.4073
MLA
Qu, F., Li, C., Yuan, B., Qi, W., Li, H., Shen, X., Zhao, G., Wang, J., Liu, Y."MicroRNA‑26a induces osteosarcoma cell growth and metastasis via the Wnt/β‑catenin pathway". Oncology Letters 11.2 (2016): 1592-1596.
Chicago
Qu, F., Li, C., Yuan, B., Qi, W., Li, H., Shen, X., Zhao, G., Wang, J., Liu, Y."MicroRNA‑26a induces osteosarcoma cell growth and metastasis via the Wnt/β‑catenin pathway". Oncology Letters 11, no. 2 (2016): 1592-1596. https://doi.org/10.3892/ol.2015.4073