MicroRNA‑26a induces osteosarcoma cell growth and metastasis via the Wnt/β‑catenin pathway

Qu,Feng; Li,Chun‑Bao; Yuan,Bang‑Tuo; Qi,Wei; Li,Hong‑Liang; Shen,Xue‑Zhen; Zhao,Gang; Wang,Jiang‑Tao; Liu,Yu‑Jie

doi:10.3892/ol.2015.4073

February-2016 Volume 11 Issue 2

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February-2016 Volume 11 Issue 2

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Article

MicroRNA‑26a induces osteosarcoma cell growth and metastasis via the Wnt/β‑catenin pathway

Authors:
- Feng Qu
- Chun‑Bao Li
- Bang‑Tuo Yuan
- Wei Qi
- Hong‑Liang Li
- Xue‑Zhen Shen
- Gang Zhao
- Jiang‑Tao Wang
- Yu‑Jie Liu
View Affiliations / Copyright

Affiliations: Department of Orthopedics, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. China, Department of Orthopedics, Chinese People's Liberation Army General Hospital, Beijing 100853, P.R. China
Pages: 1592-1596
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Published online on: December 31, 2015

https://doi.org/10.3892/ol.2015.4073
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Abstract

MicroRNAs (miRNAs/miRs) are a type of highly conserved, small non‑coding RNA that are vital to the post‑transcriptional regulation of gene expression via base pairing with target mRNA 3'‑untranslated regions (3'‑UTRs). Several studies have indicated that the abnormal expression of miRNAs occurs frequently in human osteosarcoma (OS). In the present study, the role of miR‑26a in the progression and metastasis of OS was investigated using reverse transcription‑quantitative polymerase chain reaction, a luciferase activity assay, cell viability assay, in vitro migration and invasion assays, transfection and western blot analysis. miR‑26a was upregulated in OS tissues and cell lines, and the expression of miR‑26a was indicated to affect the proliferation, migration and invasion of OS Saos‑2 cells. At the molecular level, the results showed that glycogen synthase kinase‑3β (GSK‑3β) was identified as a target of miR‑26a, and the ectopic expression of miR‑26a inhibited GSK‑3β by directly binding to the 3'‑UTR. Therefore, the expression of miR‑26a was negatively correlated with GSK‑3β in the OS tissues. These data suggest that miR‑26a is significant in the proliferation of human OS cells due to the direct regulation of Wnt/β‑catenin signaling.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

MicroRNA‑26a induces osteosarcoma cell growth and metastasis via the Wnt/β‑catenin pathway

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