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Radiation-induced expression of IER5 is dose-dependent and not associated with the clinical outcomes of radiotherapy in cervical cancer

  • Authors:
    • Hai‑Min Shi
    • Ku‑Ke Ding
    • Ping‑Kun Zhou
    • Dong‑Mei Guo
    • Dan Chen
    • Yan‑Sha Li
    • Chun‑Li Zhao
    • Chen‑Chen Zhao
    • Xin Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Gynaecology, Liaoning Cancer Hospital and Institute, Shenyang, Liaoning 110042, P.R. China, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, P.R. China, Department of Radiation Toxicology and Oncology, Beijing Institute of Radiation Medicine, Beijing 100850, P.R. China
    Copyright: © Shi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1309-1314
    |
    Published online on: January 8, 2016
       https://doi.org/10.3892/ol.2016.4086
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Abstract

This study aimed to investigate the expression of the immediate-early response 5 (IER5) gene in cervical cancer tissues and explore the association between the expression of IER5 and the clinical outcomes of radiotherapy. We collected specimens by surgery or biopsy and obtained 53 specimens from tissues after radiotherapy and 16 specimens from tissues before radiotherapy. Immunohistochemistry and western blotting were used to assess the protein expression levels of IER5. Quantitative polymerase chain reaction (qPCR) was performed to assess the mRNA expression levels of IER5. The protein and mRNA expression levels of IER5 in cervical cancer patients treated with radiation doses ≥20 Gy were significantly higher than in those treated with radiation doses <20 Gy (P<0.05) and before treatment with radiotherapy. Moreover, the expression of IER5 was significantly positively correlated with the radiation dose (immunohistochemistry: r=0.548, P=0.019; qPCR: r=0.671, P=0.002; western blotting: r=0.573, P<0.0001). Radiotherapy induced the upregulated expression of IER5 and this was dependent on the radiation dose. However, the radiation‑induced expression of IER5 was not associated with the clinical outcomes of radiotherapy in cervical cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Shi HM, Ding KK, Zhou PK, Guo DM, Chen D, Li YS, Zhao CL, Zhao CC and Zhang X: Radiation-induced expression of IER5 is dose-dependent and not associated with the clinical outcomes of radiotherapy in cervical cancer. Oncol Lett 11: 1309-1314, 2016.
APA
Shi, H., Ding, K., Zhou, P., Guo, D., Chen, D., Li, Y. ... Zhang, X. (2016). Radiation-induced expression of IER5 is dose-dependent and not associated with the clinical outcomes of radiotherapy in cervical cancer. Oncology Letters, 11, 1309-1314. https://doi.org/10.3892/ol.2016.4086
MLA
Shi, H., Ding, K., Zhou, P., Guo, D., Chen, D., Li, Y., Zhao, C., Zhao, C., Zhang, X."Radiation-induced expression of IER5 is dose-dependent and not associated with the clinical outcomes of radiotherapy in cervical cancer". Oncology Letters 11.2 (2016): 1309-1314.
Chicago
Shi, H., Ding, K., Zhou, P., Guo, D., Chen, D., Li, Y., Zhao, C., Zhao, C., Zhang, X."Radiation-induced expression of IER5 is dose-dependent and not associated with the clinical outcomes of radiotherapy in cervical cancer". Oncology Letters 11, no. 2 (2016): 1309-1314. https://doi.org/10.3892/ol.2016.4086
Copy and paste a formatted citation
x
Spandidos Publications style
Shi HM, Ding KK, Zhou PK, Guo DM, Chen D, Li YS, Zhao CL, Zhao CC and Zhang X: Radiation-induced expression of IER5 is dose-dependent and not associated with the clinical outcomes of radiotherapy in cervical cancer. Oncol Lett 11: 1309-1314, 2016.
APA
Shi, H., Ding, K., Zhou, P., Guo, D., Chen, D., Li, Y. ... Zhang, X. (2016). Radiation-induced expression of IER5 is dose-dependent and not associated with the clinical outcomes of radiotherapy in cervical cancer. Oncology Letters, 11, 1309-1314. https://doi.org/10.3892/ol.2016.4086
MLA
Shi, H., Ding, K., Zhou, P., Guo, D., Chen, D., Li, Y., Zhao, C., Zhao, C., Zhang, X."Radiation-induced expression of IER5 is dose-dependent and not associated with the clinical outcomes of radiotherapy in cervical cancer". Oncology Letters 11.2 (2016): 1309-1314.
Chicago
Shi, H., Ding, K., Zhou, P., Guo, D., Chen, D., Li, Y., Zhao, C., Zhao, C., Zhang, X."Radiation-induced expression of IER5 is dose-dependent and not associated with the clinical outcomes of radiotherapy in cervical cancer". Oncology Letters 11, no. 2 (2016): 1309-1314. https://doi.org/10.3892/ol.2016.4086
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