Open Access

Screening and identification of post-traumatic stress-related serum factors in children with Wilms' tumors

  • Authors:
    • Junjie Zhang
    • Qian Hu
    • Fei Guo
    • Lei Wang
    • Wei Zhao
    • Da Zhang
    • Heying Yang
    • Jiekai Yu
    • Lili Niu
    • Fuquan Yang
    • Shu Zheng
    • Jiaxiang Wang
  • View Affiliations

  • Published online on: January 11, 2016     https://doi.org/10.3892/ol.2016.4099
  • Pages: 1299-1304
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Wilms' tumors are one of the most common malignant, solid intra-abdominal tumors observed in children. Although potential tumor markers have been found, inflammatory cytokines interfere with the process of specific protein identification. The present study was undertaken to identify post-traumatic stress-related factors of Wilms' tumors and to verify the accuracy of early‑stage tumor‑specific serum protein markers. Serum samples were screened for differentially-expressed proteins using surface‑enhanced laser desorption/ionization‑time of flight mass spectrometry (SELDI‑TOF‑MS). Potential markers were isolated and purified using solid‑phase extraction (SPE) and SDS‑PAGE. Following enzymatic digestion of the protein samples, the peptide fragments were detected with high performance liquid chromatography‑mass spectrometry. The obtained peptide mass fingerprint was searched in the Swiss‑Prot protein sequence database via the Mascot search engine. Differentially‑expressed proteins were verified using western blot analysis. Differentially‑expressed proteins with a mass/charge of 5,816 were screened out using SELDI‑TOF‑MS, and significant differences between the tumor and control groups, and the trauma and control groups were observed. Target proteins were isolated and purified using SPE and SDS‑PAGE. Thioredoxin 1 (Trx1) was found to be differentially expressed. In the serum of children with Wilms' tumors, there was an increase in the level of the post‑traumatic stress‑related inflammatory factor, Trx1, as compared with the normal control group. Thus, the results of this study indicate that Trx1 presents a potential post‑traumatic stress-related factor of Wilms' tumors.
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February-2016
Volume 11 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhang J, Hu Q, Guo F, Wang L, Zhao W, Zhang D, Yang H, Yu J, Niu L, Yang F, Yang F, et al: Screening and identification of post-traumatic stress-related serum factors in children with Wilms' tumors. Oncol Lett 11: 1299-1304, 2016
APA
Zhang, J., Hu, Q., Guo, F., Wang, L., Zhao, W., Zhang, D. ... Wang, J. (2016). Screening and identification of post-traumatic stress-related serum factors in children with Wilms' tumors. Oncology Letters, 11, 1299-1304. https://doi.org/10.3892/ol.2016.4099
MLA
Zhang, J., Hu, Q., Guo, F., Wang, L., Zhao, W., Zhang, D., Yang, H., Yu, J., Niu, L., Yang, F., Zheng, S., Wang, J."Screening and identification of post-traumatic stress-related serum factors in children with Wilms' tumors". Oncology Letters 11.2 (2016): 1299-1304.
Chicago
Zhang, J., Hu, Q., Guo, F., Wang, L., Zhao, W., Zhang, D., Yang, H., Yu, J., Niu, L., Yang, F., Zheng, S., Wang, J."Screening and identification of post-traumatic stress-related serum factors in children with Wilms' tumors". Oncology Letters 11, no. 2 (2016): 1299-1304. https://doi.org/10.3892/ol.2016.4099