Screening and identification of post-traumatic stress-related serum factors in children with Wilms' tumors

Zhang,Junjie; Hu,Qian; Guo,Fei; Wang,Lei; Zhao,Wei; Zhang,Da; Yang,Heying; Yu,Jiekai; Niu,Lili; Yang,Fuquan; Zheng,Shu; Wang,Jiaxiang

doi:10.3892/ol.2016.4099

February-2016 Volume 11 Issue 2

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February-2016 Volume 11 Issue 2

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Article Open Access

Screening and identification of post-traumatic stress-related serum factors in children with Wilms' tumors

Authors:
- Junjie Zhang
- Qian Hu
- Fei Guo
- Lei Wang
- Wei Zhao
- Da Zhang
- Heying Yang
- Jiekai Yu
- Lili Niu
- Fuquan Yang
- Shu Zheng
- Jiaxiang Wang
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Affiliations: Department of Surgery, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Department of Nursing, Zhengzhou Railway Vocational and Technical College, Zhengzhou, Henan 450052, P.R. China, Institute of Cancer, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310000, P.R. China, Proteomic Platform, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100000, P.R. China

Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 1299-1304
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Published online on: January 11, 2016

https://doi.org/10.3892/ol.2016.4099
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Abstract

Wilms' tumors are one of the most common malignant, solid intra-abdominal tumors observed in children. Although potential tumor markers have been found, inflammatory cytokines interfere with the process of specific protein identification. The present study was undertaken to identify post-traumatic stress-related factors of Wilms' tumors and to verify the accuracy of early‑stage tumor‑specific serum protein markers. Serum samples were screened for differentially-expressed proteins using surface‑enhanced laser desorption/ionization‑time of flight mass spectrometry (SELDI‑TOF‑MS). Potential markers were isolated and purified using solid‑phase extraction (SPE) and SDS‑PAGE. Following enzymatic digestion of the protein samples, the peptide fragments were detected with high performance liquid chromatography‑mass spectrometry. The obtained peptide mass fingerprint was searched in the Swiss‑Prot protein sequence database via the Mascot search engine. Differentially‑expressed proteins were verified using western blot analysis. Differentially‑expressed proteins with a mass/charge of 5,816 were screened out using SELDI‑TOF‑MS, and significant differences between the tumor and control groups, and the trauma and control groups were observed. Target proteins were isolated and purified using SPE and SDS‑PAGE. Thioredoxin 1 (Trx1) was found to be differentially expressed. In the serum of children with Wilms' tumors, there was an increase in the level of the post‑traumatic stress‑related inflammatory factor, Trx1, as compared with the normal control group. Thus, the results of this study indicate that Trx1 presents a potential post‑traumatic stress-related factor of Wilms' tumors.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Screening and identification of post-traumatic stress-related serum factors in children with Wilms' tumors

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