Natural killer cells inhibit pulmonary metastasis of hepatocellular carcinoma in nude mice

Introduction

HCC is one of the most prevalent types of human cancer worldwide, particularly in Southeast Asia (1). The 5-year survival rate of patients with HCC remains poor, with ~600,000 mortalities each year, despite recent advances in surgical techniques and medical treatment (2). There is a lack of typical symptoms during the early stages of HCC. However, a common clinical manifestation is dull or tingling pain, as well as distension, in the hepatic area, which is the initial symptom in 50% of HCC patients. Patients may demonstrate additional symptoms, including weakness, weight loss, anorexia, abdominal distension, nausea, vomiting, fever and diarrhea. Hepatomegaly frequently occurs in terminal-stage HCC patients. There are a number of methods via which HCC may be diagnosed, including blood concentration of α-fetoprotein, color Doppler ultrasound, x-ray, computed tomography and magnetic resonance imaging (MRI) (3,4). Metastasis is one of the primary causes of the poor prognosis associated with the disease. Tumor metastasis is a difficulty that must frequently be faced when treating hepatocellular carcinoma. Decreased antitumor immunity in patients with cancer has been established to be a major factor associated with the development, progression and metastasis of several types of cancer, including hepatocellular carcinoma (HCC) (5–7). Natural killer (NK) cells serve an important role in the suppression of carcinogenesis (5). Several previous studies have demonstrated that NK cells inhibit tumor growth and that interleukin (IL)-2 is an essential cytokine for NK cell proliferation and activation (5,8). Thus, NK cells may serve as a promising treatment for HCC metastasis. However, the role of NK cells in the inhibition of hepatocellular carcinoma metastasis to the lungs has not been well characterized. In the present study, it was assessed whether NK cells, activated by IL-2, may inhibit the metastasis of hepatocellular carcinoma in vitro and in vivo.

Materials and methods

Isolation and culture of NK cells

The study protocol conformed to the ethical guidelines of the Declaration of Helsinki and was approved by the Institute Research Ethics Committee of the Xiamen Traditional Hospital (Xiamen, China). Blood samples obtained from healthy volunteers were used to separate peripheral blood mononuclear cells (PBMCs). The PBMCs were then used to isolate the NK cells using the Human NK Cell Isolation kit (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany), following the manufacturer's protocols. The NK cells were cultured in RPMI-1640 medium (HyClone; GE Healthcare Life Sciences, Logan, Utah, USA), containing 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA). Subsequently, the NK cells were activated using recombinant human IL-2 (PeproTech, Inc., Rocky Hill, NJ, USA) at a concentration of 2 pg/ml (9). The MHCC-97H cells were cultured in RPMI-1640 medium containing 10% FBS.

Detection of NK cell receptors and activated NK cell markers

Flow cytometry was used to examine the expression of the following cell surface proteins: Cluster of differentiation (CD)56 [phycoerythrin (PE)-labeled mouse anti-human CD56 clone B159; BD Biosciences, San Diego, CA, USA], CD3 [allophycocyanin (APC)-labeled mouse anti-human CD3 clone UCHTI; BD Pharmingen, San Diego, CA, USA], NKG2D [fluorescein isothiocyanate (FITC)-labeled anti-human NKG2D clone 1D11; eBioscience, Inc., San Diego, CA, USA], NKB1 (FITC-labeled anti-human NKB1 clone DX9; BD Pharmingen), perforin (FITC Perforin Reagent set containing FITC-labeled mouse anti-human perforin clone δG9 and FITC-mouse immunoglobulin (Ig)G2bκ clone 27–35; BD Pharmingen) and granzyme (FITC-labeled mouse anti-human granzyme clone GB11; BD Pharmingen). The isotype control included PE-labeled mouse IgG1κ (clone MOPC-21), APC-labeled mouse IgG1κ (clone MOPC-21), FITC-labeled mouse IgG1κ (clone MOPC-21) and PerCP-CyTM5.5 mouse IgG1κ (clone MOPC-21) (all BD Pharmingen). The dilution ratio for all antibodies was 1:100.

Proliferation assay

The NK cells were stimulated with IL-2 (2 pg/ml) for 48 h at 37°C and were then cocultured with the MHCC97-H cells, which were obtained from the Liver Cancer Institute and Zhongshan Hospital (Shanghai, China). The cells were cultured at a concentration of 2×106 cells/plate (1×106 cells/ml), using varying amounts of RPMI-1640 medium that contained 10% FBS. Following a total of 48 h, the culture medium was removed and the MHCC97-H cells were washed with phosphate-buffered saline (PBS). Subsequently, 5-bromo-2′-deoxyuridine (BrdU; Roche Diagnostics, Basel, Switzerland) was added to the MHCC97-H cells, and the cell cultures were reincubated in the RPMI-1640 medium containing 10% FBS at 37°C. Following a total of 4 h, the culture medium was removed and the cells were fixed and permeabilized, and the genomic DNA was denatured in one step through the addition of FixDenat (Roche Diagnostics) according the manufacturer's protocols. The MHCC97-H cells were collected and the BrdU-positive cells were detected with anti-BrdU antibody (PerCP-CyTM5.5-labeled mouse anti-BrdU clone 3D4; 1:100; BD Pharmingen) using flow cytometry.

Wound healing assays

Migration of the NK cells was determined by performing wound healing assays. The MHCC97-H cells were cocultured with the NK cells in 6-well plates (106 cells/well), with RPMI-1640 medium containing 10% FBS. Confluent monolayers were wounded in a straight line with a sterile plastic pipette tip, and were subsequently incubated for 72 h with the NK cells (106 cells/well), in 0.2% FBS medium containing IL-2 (2 pg/ml). In the control group, the monolayers were incubated with medium instead of NK cells. The NK cell migration was evaluated by comparing the remaining cell-free area with that of the initial wound.

Matrigel invasion assays

Cell invasion was determined using BD BioCoat Matrigel Invasion Chambers (BD Biosciences, Franklin Lakes, NJ, USA). In the NK group, 200 µl of 0.2% FBS medium, containing 5×104 MHCC97-H cells and 5×104 NK cells that had been activated with IL-2 (2 pg/ml), were added to the upper compartment of the Transwell chamber, and 600 µl of 10% FBS-containing medium was added to the lower chamber. In the control group, medium was used instead of the NK cells. Following 48 h of incubation at 37°C in a humidified CO2 incubator, non-invaded cells in the upper chamber were removed with a cotton swab, and the invaded cells were stained and counted using a microscope (×200 magnification) along the longitudinal or transverse axis, including five fields, without any overlap in the fields assessed. The mean value of the cells per field was calculated.

In vivo assessment of NK cell survival

All experimental procedures involving animals were approved by the Animal Care and Use Committee of Xiamen University (Fujian, China). Healthy nude mice were injected through the portal vein with 2×106 NK cells that were stained with carboxyfluorescein diacetate-succinimidyl ester (CFDA-SE; EnoGene Biotech, Co., Ltd., Nanjing, China), a non-fluorescent dye that passively diffuses into the cell cytoplasm. Once inside the cell, the non-fluorescent dye is cleaved by intracellular esterase to become fluorescent and can be detected under a fluorescence microscope. The NK cells were detected 2 weeks after the cell injection using the IX73-U Fluorescence Microscope (Olympus Corporation, Tokyo, Japan).

Tumor growth and metastasis assays in vivo

The study protocol was approved by the Institutional Animal Care and Use Committee of the Xiamen University (Xiamen, China), and the mice were kept in specific pathogen-free conditions (an environment without specific disease-causing microbe and parasites, including the hepatitis A and E virus; certain non-pathogenic microbes and parasites, including yeasts, were allowed to exist). A hind leg xenograft was established by subcutaneously injecting 2×106 MHCC97-H cells into 4-week-old nude mice (BALB/c nu/nu: Shanghai SLAC Laboratory Animal Co., Ltd., Shanghai, China). Following a total of 14 days, the subcutaneous tumors were removed and dissected into 1-mm3 sections, which were inoculated into the left hepatic lobe of the nude mice to establish orthotopic implantation models. Subsequently, 2×106 NK cells, which had been activated with IL-2, were injected into the orthotopic implantation models through the portal vein, prior to closing of the enterocoelia in the experimental group. In order to continuously activate the NK cells in vivo, IL-2 (1 pg/mg of body weight) was injected into the enterocelia of the orthotopic implantation models from the experimental and control groups on the 5th and 30th days post-surgery. In general, the nude mice exhibited lung metastasis 8 weeks after orthotopic implantation. Tumor growth was monitored, and the nude mice were sacrificed (anesthesia with a 5% chloral hydrate intraperitoneal injection) on the 56th day post-surgery. The control group was administered PBS instead of NK cells. The mice were sacrificed and the tumors, livers and lungs were removed, weighed, and 5-µm sections were fixed in formalin and embedded in paraffin. The liver tumors were detected through MRI, with the tumor volume estimated using the MRI data. The lung metastases were assayed according to methods described by Yang et al (10). The grading system was described as follows: Grade I, <20 tumor cells; grade II, 20–50 tumor cells; grade III, 50–100 tumor cells; and grade IV, >100 tumor cells.

Statistical analysis

Data were analyzed using commercially available software (SPSS version 13.0; SPSS, Inc., Chicago, IL, USA). Mean ± standard deviation values are used to represent replicate experimental data. Student's t-test was used for bivariate analysis, and the rank sum test was used for the assessment of multivariable data. P<0.05 was considered to indicate a statistically significant difference.

Results

Culture and purity of NK cells

A previous study demonstrated that NK cells isolated from PBMCs express high levels of CD56 and do not express CD3 (5). Therefore, the CD56+CD3− cells obtained from PBMCs were considered to be NK cells. The percentage of NK cells in the PBMCs was 10–30% (Fig. 1A) and the purity of the isolated NK cells was determined to be >95% (Fig. 1B). The isolated NK cells exhibited high activity and propagated in clusters in suspension cultures (Fig. 1C).

Figure 1. Isolation and culture of the NK cells. (A) CD56+CD3− cells were identified as the marker of the NK cells. The percentage of the NK cells in peripheral blood mononuclear cells was 10–30%. The NK cell percentage of certain samples was >30%. (B) The purity of the NK cells was >95%. (C) NK cell culture suspensions formed aggregates or clusters (magnification, ×100). NK, natural killer; PE, phycoerythrin; APC, allophycocyanin; CD, cluster of differentiation.

Role of NK cells in inhibiting MHCC97-H cell mobility in vitro

In order to examine the role of NK cells in the inhibition of hepatocellular carcinoma in vitro, the MHCC97-H cells were cocultured with or without NK cells. The BrdU stain was used to enumerate progenitive MHCC97-H cells in the presence and absence of the NK cells (Fig. 2A and B). The data demonstrated that the NK cells inhibited the MHCC97-H cell proliferation when the ratio of NK/MHCC97-H was >0.25/1, and the inhibitory effect peaked at an NK/MHCC97-H ratio of 4/1 (P<0.05). Therefore, the data indicated that NK cells inhibited the proliferation of the MHCC97-H cells in vitro.

Figure 2. In vitro assessment of the role of NK cells in inhibiting MHCC97-H cell proliferation, migration and invasion. (A) NK cells were cocultured with MHCC97-H cells in different ratios, and the progenitive MHCC97-H cells were detected with anti-BrdU antibody by flow cytometry. The BrdU-positive cells were identified as proliferative cells. (B) Inhibition rate of the indicated cells, calculated as [(the ratio of progenitive cells in the control group - the ratio of progenitive cells in the experimental group)/the ratio of progenitive cells in the control group] ×100. (C) Cell-free area of the NK and control groups (magnification, ×100). (D) Number of invading cells in the NK and control groups. (E) Histochemical analysis of the NK and control group invasion and migration (staining, crystal violet; magnification, ×200). (F) Number of migrating cells in the NK and control groups. Data are presented as the mean ± standard deviation (n=3). *P<0.05. NK, natural killer; BrdU, 5-bromo-2′-deoxyuridine.

The data also demonstrated that the mobility of the MHCC97-H cells in the wound healing assays was significantly decreased in the presence of the NK cells (Fig. 2C and D; P<0.05). Similarly, the Matrigel invasion assay data indicated that the NK cells significantly decreased migration and invasion of the MHCC97-H cells (Fig. 2E and F; P<0.05).

Role of NK cells in inhibiting MHCC97-H cell survival in vivo

In vivo cell survival experiments demonstrated that NK cells stained with CFDA-SE were localized in the liver tissue. Thus, NK cells may survive in the liver of nude mice (Fig. 3A). To examine the role of NK cells in the inhibition of hepatocellular carcinoma in vivo, the orthotopic implantation models (n=5) were injected with NK cells, and control models (n=5) were injected with PBS. The general state of health, tumor burden in the liver and cancer metastasis to the lungs were examined.

Figure 3. In vivo assessment of the role of NK cells in inhibiting MHCC97-H cell proliferation and invasion. (A) Liver sections were stained with CFDA-SE. The sections were then stained with DAPI dihydrochloride and observed with a fluorescence microscope using blue and green excitation light. Magnification, ×200. (B) In the 8th week, the liver tumors were detected using MRI. (C) Tumor volumes of the NK and control groups. (D) Histochemical analysis of the lung metastatic lesions (staining, hematoxylin and eosin; magnification, ×200). (E) Number of lung metastatic lesions in the NK and control groups. Data are presented as the mean ± standard deviation (n=3). *P<0.05. NK, natural killer; MRI, magnetic resonance imaging; DAPI, 4′,6-diamidino-2-phenylindole; CFDA-SE, carboxyfluorescein diacetate succinimidyl ester.

It was observed that the experimental and control animals survived for longer than 6 weeks. The appetite and activity of the two groups were not significantly different.

The volume and weight of the liver tumors of the experimental and control mice were not significantly different (data not shown). The metastatic lesions in the lungs were too small to be detected with MRI (Fig. 3B and C). Metastatic lesions in the lungs of the orthotopic implantation models were measured on the 56th day post-implantation. The total numbers and grades of lung metastatic lesions of the experimental group were considerably lower than those of the control group (Fig. 3D and E; P<0.05).

Effect of IL-2 stimulation on NK cell function

Previous studies have indicated that IL-2 is required for NK cell growth, proliferation and activation (11). In the present study, NK cells were cultured with or without IL-2 for 48 h using a previously described protocol (8). The expression of NK group 2, member D (NKG2D; an activating receptor for NK cells) and killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail, 1 (KIR3DL1; NKB1; an inhibitory receptor) was assessed. Additionally, the expression of perforin and granzyme was also evaluated (P<0.05). The data indicated that the NK cells exhibited moderate expression levels of NKG2D, perforin and granzyme, and that the expression of the aforementioned molecules was considerably upregulated following IL-2 stimulation (P<0.05). However, the NK cells expressed low levels of KIR3DL and the KIR3DL expression was slightly upregulated following IL-2 treatment (Fig. 4; P=0.023).

Figure 4. Role of IL-2 in activating NK cells. (A) NK cells were cultured in the presence (IL-2 group) or absence (control group) of IL-2 (2 pg/ml). The immune molecule expression was detected using flow cytometry. (B) The expression of NK cell-associated proteins on the NK cells of the control group and IL-2 group. NK, natural killer; IL, interleukin; FITC, fluorescein isothiocyanate. *P<0.05.

Discussion

NK cells are important mediators of the innate immune system, and the number of tumor-infiltrating NK cells may be used as a predictor of liver cancer prognosis (12). A previous study indicated that the function of NK cells is repressed in the liver tumor microenvironment (13). Therefore, strategies to enable NK cell function and activity are required to develop an effective treatment approach.

In the present study, the antitumor activity of NK cells was assessed. The PBMCs separated from blood consisted of 20–30% CD56+CD3− cells, which were characterized as the NK cells. The isolated CD56+CD3− cell purity was determined to be 95%. In the presence of IL-2, the NK cells demonstrated robust growth and maintained vitality. In vivo, the NK cells exhibited long-term survival in the liver of the nude mice. This finding was consistent with the findings of previous studies (8,10). NK cell dysfunction in patients with hepatocellular carcinoma has been associated with several factors (14,15), including the suppression of cytokine expression in the tumor environment (16,17). Cytokines, such as IL-12, serve an important role in the activation of NK cells in the tumor microenvironment (18). As aforementioned, IL-2 is required for the growth, proliferation and activation of NK cells. Furthermore, NK cells with repressed effector function regain cytolytic function following stimulation with IL-2 (19).

Overall, several studies have demonstrated that NK cells inhibit tumor growth. Maat et al (20) demonstrated that NK cells were involved in the prevention of uveal melanoma metastases. However, few studies have investigated the treatment efficacy of NK cell administration in the prevention of hepatocellular carcinoma metastases. Barkholt et al (8) reported that NK cells, along with IL-2, may be safely administered to patients with hepatocellular carcinoma. In the present in vitro study, the data indicated that IL-2 activated the NK cells, and therefore inhibited MHCC97-H proliferation, migration and invasion. Furthermore, the in vivo data demonstrated that the NK cells inhibited pulmonary metastases of hepatocellular cancer. However, the tumor burden in the livers obtained from the control and NK groups was not significantly different. The similarity in tumor burden may be due to low levels of tumor necrosis and the poor nutritional intake by the nude mice in the last weeks of the experiments.

The mechanisms underlying the NK-mediated inhibition of tumor invasion and tumor metastasis have not been well characterized. As aforementioned, the cytokines secreted by NK cells may be crucial in activating antitumor immunity. For example, NK cells are a major producer of interferon-γ, which is critical for the IL-12-induced antitumor effect (21). Furthermore, NK cells may mediate antitumor responses via direct contact with tumor cells or other immune cells. The contact between NK cells and tumor cells may lead to tumor cell immunoediting through cell surface molecular interactions (22,23).

Previous studies have demonstrated that NK cell receptors and their corresponding ligands serve an important role in liver diseases (24,25). NKG2D is an important activating receptor of NK cells, and NKG2D expression has been directly associated with hepatocellular carcinoma prognosis (26). Ul16 binding protein is an NKG2D ligand and is expressed on tumor cells. The absence of Ul16 binding protein expression has been identified as an independent factor for recurrent hepatocellular carcinoma (27). NK cell cytotoxicity is mediated through the NKG2D-major histocompatability complex (MHC) class I-related chain A/B interaction (28). In the present study, the expression of NKG2D increased significantly following incubation with IL-2 and the expression of NKB1, an inhibitory receptor, was elevated moderately. The expression of cell markers for activated NK cells (granzyme and perforin) increased significantly. Overall, the data indicate that NK cells exert inhibitory effects on tumor cell proliferation, migration and invasion.

Killer immunoglobulin receptors (KIRs) are NK cell receptors that are associated with liver cancer incidence. KIRs are involved in the regulation of NK cell activation through recognition and binding with specific human leukocyte antigen (HLA) class I allotypes. Thus, the type of HLAs expressed on tumor cells may determine the antitumor efficacy of the NK cells (29). NKB1 (KIR3DL1) is a member of the KIR family. The interaction of NKB1 with specific HLA-B antigens on a target cell inhibits cell-mediated cytotoxicity, possibly by delivering a negative signal that prevents NK cell activation. Therefore, the interactions between MHC I and KIRs may regulate NK responses to antigenic challenge (30).

The mechanisms underlying the antitumor and anti-metastatic activity of NK cells have not been well characterized. Few studies have indicated that NK cells may mediate the removal of micro-metastatic cells from circulation and attack metastatic cells present inside organs. Therefore, methods to further improve the antitumor functions of NK cells are of considerable interest. The role of signaling pathways and gene expression in the development of NK cells has been previously investigated. One study demonstrated that activation of the Notch signaling pathway supports the development of cytokine-producing NK cells that are hyporesponsive and have low receptor and poor effector functions (31). Furthermore, microRNA-30c-1 has been identified to strengthen NK cell cytotoxicity against hepatocellular carcinoma cells by regulating the expression of the transcription factor homeobox containing 1 (32). When combined, these studies indicate that the NK cell antitumor activity may be improved by regulating the signal transduction pathways or the gene expression of NK cells.

In conclusion, in the present study, it was demonstrated that NK cells inhibited the proliferation, migration and invasion of the MHCC97-H cells in vitro, and that the NK cells survived in the livers of the nude mice. Furthermore, the NK cells inhibited lung metastasis of hepatocellular carcinoma in vivo. However, the NK cells did not inhibit tumor growth in the liver of the nude mice. The data also indicated that IL-2 may enhance NK cell-mediated metastasis inhibition by inducing the expression of immunologically relevant molecules. However, further investigation is required to identify the mechanisms that enable NK cells to inhibit cancer metastasis from the liver to the lungs.

Acknowledgements

This study was supported by grants from the National Natural Science Foundation of China (no. 81171976) and the Fujian University of Traditional Chinese Medicine (no. XB2015039).

References