Downregulation of LSD1 suppresses the proliferation, tumorigenicity and invasion of papillary thyroid carcinoma K1 cells

  • Authors:
    • Ling‑Ling Kong
    • Dong‑Mei Man
    • Tian Wang
    • Guo‑An Zhang
    • Wen Cui
  • View Affiliations

  • Published online on: February 18, 2016     https://doi.org/10.3892/ol.2016.4244
  • Pages: 2475-2480
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Abstract

The present study aimed to evaluate the effects of lysine‑specific demethylase 1 (LSD1) downregulation, induced by small interfering RNA (siRNA) transfection, on the proliferation, colony formation, migration and invasion of the papillary thyroid carcinoma K1 cell line. The siRNA targeting LSD1 and scrambled non‑targeting siRNA were each transfected into papillary thyroid carcinoma K1 cells. Downregulation of LSD1 mRNA and protein level was evaluated by reverse transcription‑quantitative polymerase chain reaction, and immunocytochemical (ICC) analysis and western blotting, respectively. A Cell Counting kit‑8 assay was applied to estimate the effect of LSD1‑siRNA on cell growth. Migration and invasion abilities were estimated by Transwell chamber assay. A soft agar colony formation assay was performed to estimate the effect of LSD1‑siRNA on tumorigenicity in vitro. ICC data showed that LSD1 protein was strongly expressed in the blank and control K1 cells compared with the LSD1‑siRNA cells (F=15.192, P<0.01). Compared with the control cells, cells transfected with siRNA targeting LSD1 exhibited significant downregulation of LSD1 mRNA (t=6.845, P<0.01) and protein (F=53.764, P<0.01) levels. siRNA targeting LSD1 also downregulated cell proliferation following transfection for 24, 48 and 72 h (t=4.777, P<0.001; t=3.302, P=0.003; and t=3.017, P=0.006, respectively). Compared with the control group, the amount of cell invasion was gradually reduced in the LSD1‑siRNA group (t=12.301, P<0.01). The number of migrating cells was significantly higher in the negative control group compared with the LSD1‑siRNA group (t=7.911, P<0.01), and the ability of colony formation in the LSD1‑siRNA cells was notably reduced in the soft agar formation assay (t=3.612, P=0.005). siRNA targeting LSD1 efficiently inhibits the proliferation, colony formation, migration and invasion of papillary thyroid carcinoma K1 cells.
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April-2016
Volume 11 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Kong LL, Man DM, Wang T, Zhang GA and Cui W: Downregulation of LSD1 suppresses the proliferation, tumorigenicity and invasion of papillary thyroid carcinoma K1 cells. Oncol Lett 11: 2475-2480, 2016
APA
Kong, L., Man, D., Wang, T., Zhang, G., & Cui, W. (2016). Downregulation of LSD1 suppresses the proliferation, tumorigenicity and invasion of papillary thyroid carcinoma K1 cells. Oncology Letters, 11, 2475-2480. https://doi.org/10.3892/ol.2016.4244
MLA
Kong, L., Man, D., Wang, T., Zhang, G., Cui, W."Downregulation of LSD1 suppresses the proliferation, tumorigenicity and invasion of papillary thyroid carcinoma K1 cells". Oncology Letters 11.4 (2016): 2475-2480.
Chicago
Kong, L., Man, D., Wang, T., Zhang, G., Cui, W."Downregulation of LSD1 suppresses the proliferation, tumorigenicity and invasion of papillary thyroid carcinoma K1 cells". Oncology Letters 11, no. 4 (2016): 2475-2480. https://doi.org/10.3892/ol.2016.4244