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Article

Hyperoside and rutin of Nelumbo nucifera induce mitochondrial apoptosis through a caspase-dependent mechanism in HT-29 human colon cancer cells

  • Authors:
    • Tae Eun Guon
    • Ha Sook Chung
  • View Affiliations / Copyright

    Affiliations: Department of Food and Nutrition, College of Natural Sciences, Duksung Women's University, Seoul 132‑714, Republic of Korea
  • Pages: 2463-2470
    |
    Published online on: February 18, 2016
       https://doi.org/10.3892/ol.2016.4247
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Abstract

The present study demonstrates the mechanism of 2 flavonol glycosides, hyperoside and rutin, in the induction of apoptosis in HT-29 human colon cancer cells through the bioactivity-guided fractionation and isolation method. The chemical structure of hyperoside and rutin, isolated from the roots of Nelumbo nucifera, were established using extensive 1- and 2-dimensional nuclear magnetic resonance experiments and absolute high resolution fast-atom bombardment mass spectrometry, ultraviolet‑visible and Fourier transform infrared spectroscopy spectral analytical methods. The treatment of HT‑29 colon cancer cells with hyperoside and rutin significantly decreased cell viability in a dose‑dependent manner. The concomitant activation of the mitochondria-dependent apoptotic pathway of hyperoside and rutin occurred via modulation of Bcl-2-associated X protein and B-cell lymphoma 2 expression, resulting in the activation of cleaved caspases-3, -8 and -9 and cleaved poly‑(ADP‑ribose) polymerase. The findings of the present study indicate that hyperoside and rutin induce apoptosis in HT‑29 human colon cancer cells, and that this phenomenon is mediated via the death receptor‑mediated and mitochondria‑mediated apoptotic pathways. These results suggest that hyperoside and rutin may be useful in the development of a colon cancer therapy protocol.
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Copy and paste a formatted citation
Spandidos Publications style
Guon TE and Chung HS: Hyperoside and rutin of Nelumbo nucifera induce mitochondrial apoptosis through a caspase-dependent mechanism in HT-29 human colon cancer cells. Oncol Lett 11: 2463-2470, 2016.
APA
Guon, T.E., & Chung, H.S. (2016). Hyperoside and rutin of Nelumbo nucifera induce mitochondrial apoptosis through a caspase-dependent mechanism in HT-29 human colon cancer cells. Oncology Letters, 11, 2463-2470. https://doi.org/10.3892/ol.2016.4247
MLA
Guon, T. E., Chung, H. S."Hyperoside and rutin of Nelumbo nucifera induce mitochondrial apoptosis through a caspase-dependent mechanism in HT-29 human colon cancer cells". Oncology Letters 11.4 (2016): 2463-2470.
Chicago
Guon, T. E., Chung, H. S."Hyperoside and rutin of Nelumbo nucifera induce mitochondrial apoptosis through a caspase-dependent mechanism in HT-29 human colon cancer cells". Oncology Letters 11, no. 4 (2016): 2463-2470. https://doi.org/10.3892/ol.2016.4247
Copy and paste a formatted citation
x
Spandidos Publications style
Guon TE and Chung HS: Hyperoside and rutin of Nelumbo nucifera induce mitochondrial apoptosis through a caspase-dependent mechanism in HT-29 human colon cancer cells. Oncol Lett 11: 2463-2470, 2016.
APA
Guon, T.E., & Chung, H.S. (2016). Hyperoside and rutin of Nelumbo nucifera induce mitochondrial apoptosis through a caspase-dependent mechanism in HT-29 human colon cancer cells. Oncology Letters, 11, 2463-2470. https://doi.org/10.3892/ol.2016.4247
MLA
Guon, T. E., Chung, H. S."Hyperoside and rutin of Nelumbo nucifera induce mitochondrial apoptosis through a caspase-dependent mechanism in HT-29 human colon cancer cells". Oncology Letters 11.4 (2016): 2463-2470.
Chicago
Guon, T. E., Chung, H. S."Hyperoside and rutin of Nelumbo nucifera induce mitochondrial apoptosis through a caspase-dependent mechanism in HT-29 human colon cancer cells". Oncology Letters 11, no. 4 (2016): 2463-2470. https://doi.org/10.3892/ol.2016.4247
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