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Article Open Access

CD44 promotes the migration of bone marrow-derived mesenchymal stem cells toward glioma

  • Authors:
    • Qiang Yin
    • Yang‑Yang Zhou
    • Peng Wang
    • Li Ma
    • Peng Li
    • Xiao‑Guang Wang
    • Chun‑Hua She
    • Wen‑Liang Li
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery and Neuro‑Oncology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin Key Laboratory of Cancer Prevention and Therapy, Tianjin 300060, P.R. China, Division of Pulmonary Medicine, Tianjin Institute of Respiratory Diseases, Tianjin Haihe Hospital, Tianjin 300222, P.R. China
    Copyright: © Yin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2353-2358
    |
    Published online on: February 24, 2016
       https://doi.org/10.3892/ol.2016.4270
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Abstract

Previous in vivo and in vitro studies have shown that human mesenchymal stem cells (MSCs) exhibit tropism for gliomas. However, the mechanism underlying this directed migration remains unclear. The aim of the present study was to investigate the possible mechanism underlying platelet-derived growth factor‑BB (PDGF‑BB)-induced chemotactic migration of bone marrow-derived MSCs (BMSCs) toward glioma. Rat glioma C6 cell-conditioned medium was utilized to evaluate the chemotactic response of BMSCs toward glioma using an in vitro migration assay. Recombinant rat PDGF-BB was added to C6 cell‑conditioned medium to assess its effect on the tropism of BMSCs. The effect of PDGF‑BB on the expression levels of cluster of differentiation (CD)44 in BMSCs was evaluated by reverse transcription‑polymerase chain reaction (RT‑PCR) and immunofluorescence assays. The results revealed that chemotactic migration was induced in BMSCs by rat glioma C6 cell‑conditioned medium, which was enhanced by PDGF-BB treatment in a dose‑dependent manner. Furthermore, RT‑PCR and immunofluorescence assays showed that CD44 expression was upregulated in BMSCs following treatment with 40 ng/ml PDGF‑BB for 12 h. Additionally, 3‑h pretreatment with the anti‑CD44 neutralizing antibody OX‑50 was observed to attenuate the tropism of BMSCs toward glioma in the presence or absence of PDGF‑BB. The results of the present study indicate that CD44 mediates the tropism of BMSCs toward glioma, and PDGF‑BB promotes the migration of BMSCs toward glioma via the upregulation of CD44 expression in BMSCs. These findings suggest CD44 inhibition may be a potential therapeutic target for the treatment of glioma.
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Copy and paste a formatted citation
Spandidos Publications style
Yin Q, Zhou YY, Wang P, Ma L, Li P, Wang XG, She CH and Li WL: CD44 promotes the migration of bone marrow-derived mesenchymal stem cells toward glioma. Oncol Lett 11: 2353-2358, 2016.
APA
Yin, Q., Zhou, Y., Wang, P., Ma, L., Li, P., Wang, X. ... Li, W. (2016). CD44 promotes the migration of bone marrow-derived mesenchymal stem cells toward glioma. Oncology Letters, 11, 2353-2358. https://doi.org/10.3892/ol.2016.4270
MLA
Yin, Q., Zhou, Y., Wang, P., Ma, L., Li, P., Wang, X., She, C., Li, W."CD44 promotes the migration of bone marrow-derived mesenchymal stem cells toward glioma". Oncology Letters 11.4 (2016): 2353-2358.
Chicago
Yin, Q., Zhou, Y., Wang, P., Ma, L., Li, P., Wang, X., She, C., Li, W."CD44 promotes the migration of bone marrow-derived mesenchymal stem cells toward glioma". Oncology Letters 11, no. 4 (2016): 2353-2358. https://doi.org/10.3892/ol.2016.4270
Copy and paste a formatted citation
x
Spandidos Publications style
Yin Q, Zhou YY, Wang P, Ma L, Li P, Wang XG, She CH and Li WL: CD44 promotes the migration of bone marrow-derived mesenchymal stem cells toward glioma. Oncol Lett 11: 2353-2358, 2016.
APA
Yin, Q., Zhou, Y., Wang, P., Ma, L., Li, P., Wang, X. ... Li, W. (2016). CD44 promotes the migration of bone marrow-derived mesenchymal stem cells toward glioma. Oncology Letters, 11, 2353-2358. https://doi.org/10.3892/ol.2016.4270
MLA
Yin, Q., Zhou, Y., Wang, P., Ma, L., Li, P., Wang, X., She, C., Li, W."CD44 promotes the migration of bone marrow-derived mesenchymal stem cells toward glioma". Oncology Letters 11.4 (2016): 2353-2358.
Chicago
Yin, Q., Zhou, Y., Wang, P., Ma, L., Li, P., Wang, X., She, C., Li, W."CD44 promotes the migration of bone marrow-derived mesenchymal stem cells toward glioma". Oncology Letters 11, no. 4 (2016): 2353-2358. https://doi.org/10.3892/ol.2016.4270
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