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Article Open Access

Calcium efflux from the endoplasmic reticulum regulates cisplatin-induced apoptosis in human cervical cancer HeLa cells

Corrigendum in: /10.3892/ol.2025.15020
  • Authors:
    • Luyan Shen
    • Naiyan Wen
    • Meihui Xia
    • Yu Zhang
    • Weimin Liu
    • Ye Xu
    • Liankun Sun
  • View Affiliations / Copyright

    Affiliations: Department of Pathophysiology, Basic College of Medicine, Jilin University, Changchun, Jilin 130021, P.R. China, Department of Obstetrics and Gynecology, First Hospital, Jilin University, Changchun, Jilin 130021, P.R. China, Department of Interventional Radiology, Renmin Hospital, Jilin, Jilin 132013, P.R. China, Medical Research Laboratory, Jilin Medical College, Jilin, Jilin 132013, P.R. China
    Copyright: © Shen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2411-2419
    |
    Published online on: February 25, 2016
       https://doi.org/10.3892/ol.2016.4278
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Abstract

The function of calcium efflux from the endoplasmic reticulum (ER) in cisplatin-induced apoptosis is not fully understood in cancer cells. The present study used western blot analysis, flow cytometry, immunofluorescence and 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay to investigate calcium signaling in human cervical cancer cells exposed to cisplatin. In the present study, treatment with cisplatin increased free Ca2+ levels in the cytoplasm and mitochondria of human cervical cancer HeLa cells, which further triggers the mitochondria‑mediated and ER stress‑associated apoptosis pathways. Notably, blocking calcium signaling using the calcium chelating agent bis-(o-aminophenoxy)ethane‑N,N,N',N'-tetra-acetic acid acetoxymethyl ester inhibited cisplatin-induced apoptosis via downregulation of the calcium‑dependent proteases, the calpains, and innate apoptosis proteins, such as caspsae‑3, caspase‑4 and C/EBP homologous protein (CHOP). In addition, use of the inositol triphosphate receptor inhibitor, 2-aminoethyl diphenylborinate, to inhibit calcium efflux from the ER resulted in similar effects. This data indicated that calcium efflux from the ER plays a significant role in cisplatin‑induced apoptosis in human cervical cancer HeLa cells, which provides further mechanistic insights into the tumor cell‑killing effect of cisplatin and potential therapeutic strategies to improve cisplatin chemotherapy.
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Copy and paste a formatted citation
Spandidos Publications style
Shen L, Wen N, Xia M, Zhang Y, Liu W, Xu Y and Sun L: Calcium efflux from the endoplasmic reticulum regulates cisplatin-induced apoptosis in human cervical cancer HeLa cells Corrigendum in /10.3892/ol.2025.15020. Oncol Lett 11: 2411-2419, 2016.
APA
Shen, L., Wen, N., Xia, M., Zhang, Y., Liu, W., Xu, Y., & Sun, L. (2016). Calcium efflux from the endoplasmic reticulum regulates cisplatin-induced apoptosis in human cervical cancer HeLa cells Corrigendum in /10.3892/ol.2025.15020. Oncology Letters, 11, 2411-2419. https://doi.org/10.3892/ol.2016.4278
MLA
Shen, L., Wen, N., Xia, M., Zhang, Y., Liu, W., Xu, Y., Sun, L."Calcium efflux from the endoplasmic reticulum regulates cisplatin-induced apoptosis in human cervical cancer HeLa cells Corrigendum in /10.3892/ol.2025.15020". Oncology Letters 11.4 (2016): 2411-2419.
Chicago
Shen, L., Wen, N., Xia, M., Zhang, Y., Liu, W., Xu, Y., Sun, L."Calcium efflux from the endoplasmic reticulum regulates cisplatin-induced apoptosis in human cervical cancer HeLa cells Corrigendum in /10.3892/ol.2025.15020". Oncology Letters 11, no. 4 (2016): 2411-2419. https://doi.org/10.3892/ol.2016.4278
Copy and paste a formatted citation
x
Spandidos Publications style
Shen L, Wen N, Xia M, Zhang Y, Liu W, Xu Y and Sun L: Calcium efflux from the endoplasmic reticulum regulates cisplatin-induced apoptosis in human cervical cancer HeLa cells Corrigendum in /10.3892/ol.2025.15020. Oncol Lett 11: 2411-2419, 2016.
APA
Shen, L., Wen, N., Xia, M., Zhang, Y., Liu, W., Xu, Y., & Sun, L. (2016). Calcium efflux from the endoplasmic reticulum regulates cisplatin-induced apoptosis in human cervical cancer HeLa cells Corrigendum in /10.3892/ol.2025.15020. Oncology Letters, 11, 2411-2419. https://doi.org/10.3892/ol.2016.4278
MLA
Shen, L., Wen, N., Xia, M., Zhang, Y., Liu, W., Xu, Y., Sun, L."Calcium efflux from the endoplasmic reticulum regulates cisplatin-induced apoptosis in human cervical cancer HeLa cells Corrigendum in /10.3892/ol.2025.15020". Oncology Letters 11.4 (2016): 2411-2419.
Chicago
Shen, L., Wen, N., Xia, M., Zhang, Y., Liu, W., Xu, Y., Sun, L."Calcium efflux from the endoplasmic reticulum regulates cisplatin-induced apoptosis in human cervical cancer HeLa cells Corrigendum in /10.3892/ol.2025.15020". Oncology Letters 11, no. 4 (2016): 2411-2419. https://doi.org/10.3892/ol.2016.4278
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