Differential expression of TYRP1 in adult human retinal pigment epithelium and uveal melanoma cells

Qiu,Chun; Li,Peng; Bi,Jianjun; Wu,Qing; Lu,Linna; Qian,Guanxiang; Jia,Renbing; Jia,Rong

doi:10.3892/ol.2016.4280

Differential expression of TYRP1 in adult human retinal pigment epithelium and uveal melanoma cells

Authors:
- Chun Qiu
- Peng Li
- Jianjun Bi
- Qing Wu
- Linna Lu
- Guanxiang Qian
- Renbing Jia
- Rong Jia
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Affiliations: School of Life Science, Anhui University, Hefei, Anhui 230601, P.R. China, Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200011, P.R. China
Published online on: February 25, 2016 https://doi.org/10.3892/ol.2016.4280
Pages: 2379-2383
Copyright: © Qiu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Uveal melanoma (UM) is the most frequently occurring primary intraocular malignancy in adults. Tyrosinase (TYR) is a copper‑containing enzyme and a type I membrane protein that is involved in the generation of melanin, the main pigment in vertebrates. TYR‑related protein 1 (TYRP1) is regarded to have a crucial role in the immunotherapy of melanoma. As biomarkers, the TYR‑related proteins, TYRP1 and TYRP2, exhibit specific expression in melanocytes, while also contributing to melanin synthesis within melanosomes. In the present study, the differential expression of TYRP1 was investigated at the mRNA, protein and morphological levels in four human UM cell lines (SP6.5, OM431, OCM1 and OCM290) and the human retinal pigment epithelium (RPE) cell line, using polymerase chain reaction, western blotting, immunocytochemistry and immunofluorescence staining. It was found that SP6.5 cells expressed the highest level of TYRP1, in comparison to SP6.5 OCM1 and OM431 cells, which produced less TYRP1, and OCM290 cells, which produced almost no TYRP1. No TYRP1 protein expression was identified in the RPE cell line. These findings indicate the potential use of TYRP1 in the development of therapy for UM.

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