Open Access

ASF-4-1 fibroblast-rich culture increases chemoresistance and mTOR expression of pancreatic cancer BxPC-3 cells at the invasive front in vitro, and promotes tumor growth and invasion in vivo

  • Authors:
    • Masaya Fujiwara
    • Kazuki Kanayama
    • Yoshifumi S. Hirokawa
    • Taizo Shiraishi
  • View Affiliations

  • Published online on: March 1, 2016     https://doi.org/10.3892/ol.2016.4289
  • Pages: 2773-2779
  • Copyright: © Fujiwara et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Pancreatic cancer develops dense stromal tissue through the desmoplastic reaction. The aim of the present study was to assess the effects of a fibroblast-rich environment on the malignant potential of pancreatic cancer. Cells from the human pancreatic cancer cell line BxPC‑3 were mixed at a ratio of 1:3 (fibroblast‑rich) or 1:1 (fibroblast-poor) with cells from the human skin fibroblast line ASF-4-1. In the fibroblast‑rich co‑culture, tumor budding was observed and BxPC-3 cells were found to be more resistant to gemcitabine than those in the fibroblast‑poor co‑culture. Immunohistochemistry revealed that the expression of mammalian target of rapamycin was increased at the invasive front of fibroblast-rich co-cultures. In addition, in mouse xenografts of fibroblast-rich co‑cultures, tumors were larger and had a higher Ki‑67 index compared with that of the fibroblast-poor co-culture xenografts. These results indicate that fibroblast-rich co-cultures may promote the malignant potential of the pancreatic cancer cell line BxPC-3, both in vitro and in vivo.
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April-2016
Volume 11 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Fujiwara M, Kanayama K, Hirokawa YS and Shiraishi T: ASF-4-1 fibroblast-rich culture increases chemoresistance and mTOR expression of pancreatic cancer BxPC-3 cells at the invasive front in vitro, and promotes tumor growth and invasion in vivo. Oncol Lett 11: 2773-2779, 2016
APA
Fujiwara, M., Kanayama, K., Hirokawa, Y.S., & Shiraishi, T. (2016). ASF-4-1 fibroblast-rich culture increases chemoresistance and mTOR expression of pancreatic cancer BxPC-3 cells at the invasive front in vitro, and promotes tumor growth and invasion in vivo. Oncology Letters, 11, 2773-2779. https://doi.org/10.3892/ol.2016.4289
MLA
Fujiwara, M., Kanayama, K., Hirokawa, Y. S., Shiraishi, T."ASF-4-1 fibroblast-rich culture increases chemoresistance and mTOR expression of pancreatic cancer BxPC-3 cells at the invasive front in vitro, and promotes tumor growth and invasion in vivo". Oncology Letters 11.4 (2016): 2773-2779.
Chicago
Fujiwara, M., Kanayama, K., Hirokawa, Y. S., Shiraishi, T."ASF-4-1 fibroblast-rich culture increases chemoresistance and mTOR expression of pancreatic cancer BxPC-3 cells at the invasive front in vitro, and promotes tumor growth and invasion in vivo". Oncology Letters 11, no. 4 (2016): 2773-2779. https://doi.org/10.3892/ol.2016.4289