Prognostic significance of claudin-1 and cyclin B1 protein expression in patients with hypopharyngeal squamous cell carcinoma

Li,Wujie; Dong,Qing; Li,Lei; Zhang,Zhenlei; Cai,Xiaolan; Pan,Xinliang

doi:10.3892/ol.2016.4333

Prognostic significance of claudin-1 and cyclin B1 protein expression in patients with hypopharyngeal squamous cell carcinoma

Authors:
- Wujie Li
- Qing Dong
- Lei Li
- Zhenlei Zhang
- Xiaolan Cai
- Xinliang Pan
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Affiliations: Department of Otolaryngology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China, Department of Pediatrics, Tai'an City Central Hospital, Tai'an, Shandong 271000, P.R. China, Department of Gastroenterology, Tai'an City Central Hospital, Tai'an, Shandong 271000, P.R. China, Department of Otolaryngology, Tai'an City Central Hospital, Tai'an, Shandong 271000, P.R. China
Published online on: March 16, 2016 https://doi.org/10.3892/ol.2016.4333
Pages: 2995-3002
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Claudin-l and cyclin B1 are abnormally expressed in certain malignancies, but their expression in hypopharyngeal squamous cell carcinoma (HSCC) has not been reported thus far. Studying the expression levels of claudin-1 and cylin B1 in HSCC tissues and their association with clinical stage, pathological grade and prognosis in patients with HSCC may provide a theoretical basis and guide future research on HSCC targeted therapy. The protein expression levels of the above two biomarkers was immunohistochemically detected in 97 HSCC cases and 90 matched adjacent tissue samples. The correlation between the expression levels of claudin‑1 and cylin B1 and the patients' clinical parameters was analyzed via Pearson's χ2 test, while survival analysis was performed using a log‑rank test. The results of the current study revealed that claudin‑1 and cyclin B1 were highly expressed in HSCC tissues, and the expression of claudin‑1 was associated with tumor differentiation degree and lymph node metastasis, while cyclin B1 expression was associated with tumor differentiation degree. Furthermore, Kaplan‑Meier analysis revealed that claudin‑1 expression correlated with survival (P=0.003), and the expression levels of claudin‑1 and cyclin B1 were observed to be positively correlated, in patients with HSCC. Cyclin B1 and claudin‑1 exhibited an elevated expression in HSCC specimens, thus suggesting their use as tumor markers. Therefore, the joint detection of claudin‑1 and cyclin B1 may aid to guide cancer therapy and to determine prognosis in HSCC. Furthermore, claudin-1 may be used as an HSCC-monitoring index, and may serve as a therapeutic target.

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