Re-sensitization of radiation resistant colorectal cancer cells to radiation through inhibition of AMPK pathway

Jin,Hongyong; Gao,Sujie; Guo,Huiling; Ren,Shengnan; Ji,Fujian; Liu,Zhuo; Chen,Xuebo

doi:10.3892/ol.2016.4339

May-2016 Volume 11 Issue 5

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May-2016 Volume 11 Issue 5

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Article

Re-sensitization of radiation resistant colorectal cancer cells to radiation through inhibition of AMPK pathway

Authors:
- Hongyong Jin
- Sujie Gao
- Huiling Guo
- Shengnan Ren
- Fujian Ji
- Zhuo Liu
- Xuebo Chen
View Affiliations / Copyright

Affiliations: Department of General Surgery, China Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China, Department of Anesthesia, China Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China
Pages: 3197-3201
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Published online on: March 16, 2016

https://doi.org/10.3892/ol.2016.4339
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Abstract

Radiotherapy (RT) is commonly used to treat multi-tumors to attenuate the risk of recurrence. Despite impressive initial clinical responses, a large proportion of patients experience resistance to RT. Therefore, identification of functionally relevant biomarkers would be beneficial for radioresistant patients. Adenosine monophosphate-activated kinase (AMPK) is recognized as a mediator of tumor suppressor gene function. In the present study, radio-sensitive and ‑resistant colon cancer patient samples were compared and the AMPK pathway was observed to be highly activated in radioresistant patients. In addition, the protein and mRNA levels of AMPK were upregulated in radioresistant colon cancer cells in comparison to radiosensitive colon cancer cells. The present study provides evidence that activation of AMPK by metformin contributes to radioresistance. Inhibition of AMPK by either small interfering RNA or Compound C, which is a specific inhibitor of AMPK, re‑sensitized radiation resistant cells. The data presented indicates a synergistic effect on radiation resistant cancer cells by the combination of Compound C and radiation. In summary, the present study proposes that inhibition of the AMPK pathway is a potential strategy for reversing radiation resistance and may contribute to the development of therapeutic anticancer drugs.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Re-sensitization of radiation resistant colorectal cancer cells to radiation through inhibition of AMPK pathway

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