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Article Open Access

Co-expression of pregnane X receptor and ATP-binding cassette sub-family B member 1 in peripheral blood: A prospective indicator for drug resistance prediction in non-small cell lung cancer

  • Authors:
    • Qingnuan Kong
    • Zenglei Han
    • Xiaoli Zuo
    • Hongjun Wei
    • Weiqing Huang
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Qingdao Municipal Hospital, Affiliated to Medical College of Qingdao University, Qingdao, Shandong 266071, P.R. China, Department of Pathology, Qingdao Hiser Hospital, Qingdao, Shandong 266011, P.R. China
    Copyright: © Kong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3033-3039
    |
    Published online on: March 22, 2016
       https://doi.org/10.3892/ol.2016.4369
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Abstract

The aim of the present study was to investigate the protein expression profiling of pregnane X receptor (PXR) and ATP-binding cassette sub-family B member 1 (ABCB1; also known as MDR1 or P‑gp), present in the peripheral blood mononuclear cells (PBMCs) and cancerous tissues of cases of non‑small cell lung cancer (NSCLC). Furthermore, the study aimed to assess the feasibility of predicting drug resistance through the medium of PBMCs. Of the subjects included in the study, 37 were histopathologically diagnosed with NSCLC and 17 were control patients without cancer. ThinPrep liquid‑based smears with cytosine were applied in the examination of the PBMCs and proved quite effective in preserving the morphology and surface antigens of the lymphocytes. Measurements of expression levels in the PBMCs and cancerous tissues were obtained by immunohistochemical means. The results showed that, with the exception of the selective PXR expression in the normal lung tissues, the two types of proteins existed extensively throughout the PBMCs, normal tissues and tumors. Among the cancer patients, prior to chemotherapy, a significant rise in ABCB1 expression could be observed in the PBMCs, together with a similar rise in ABCB1 and PXR expression in the tumor specimens. Marked upregulation of the two proteins was detected in the PBMCs following 1 cycle of first‑line chemotherapy. ABCB1 expression, correlated with PXR, persisted mostly in the PBMCs and tissue samples. When bound to and activated by ligands, PXR translocates from the cytoplasm to the nucleus of the cells. PXR subsequently binds to its DNA response elements as a heterodimer with the retinoid X receptor. A PXR translocation of moderate or low differentiation was identified in 3 cases of adenocarcinoma, which were co‑expressing the two genes in the PBMCs prior to chemotherapy. During follow‑up visits, tumor recurrence was observed within 3 months in 5 cases, which were characterized by PXR translocation. These findings indicate that the combined expression of PXR and ABCB1 in PBMCs may be used as a prospective indicator in diagnosis prior to histopathological diagnosis, and therefore may function as a novel biomarker for the prediction of drug resistance.
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Copy and paste a formatted citation
Spandidos Publications style
Kong Q, Han Z, Zuo X, Wei H and Huang W: Co-expression of pregnane X receptor and ATP-binding cassette sub-family B member 1 in peripheral blood: A prospective indicator for drug resistance prediction in non-small cell lung cancer. Oncol Lett 11: 3033-3039, 2016.
APA
Kong, Q., Han, Z., Zuo, X., Wei, H., & Huang, W. (2016). Co-expression of pregnane X receptor and ATP-binding cassette sub-family B member 1 in peripheral blood: A prospective indicator for drug resistance prediction in non-small cell lung cancer. Oncology Letters, 11, 3033-3039. https://doi.org/10.3892/ol.2016.4369
MLA
Kong, Q., Han, Z., Zuo, X., Wei, H., Huang, W."Co-expression of pregnane X receptor and ATP-binding cassette sub-family B member 1 in peripheral blood: A prospective indicator for drug resistance prediction in non-small cell lung cancer". Oncology Letters 11.5 (2016): 3033-3039.
Chicago
Kong, Q., Han, Z., Zuo, X., Wei, H., Huang, W."Co-expression of pregnane X receptor and ATP-binding cassette sub-family B member 1 in peripheral blood: A prospective indicator for drug resistance prediction in non-small cell lung cancer". Oncology Letters 11, no. 5 (2016): 3033-3039. https://doi.org/10.3892/ol.2016.4369
Copy and paste a formatted citation
x
Spandidos Publications style
Kong Q, Han Z, Zuo X, Wei H and Huang W: Co-expression of pregnane X receptor and ATP-binding cassette sub-family B member 1 in peripheral blood: A prospective indicator for drug resistance prediction in non-small cell lung cancer. Oncol Lett 11: 3033-3039, 2016.
APA
Kong, Q., Han, Z., Zuo, X., Wei, H., & Huang, W. (2016). Co-expression of pregnane X receptor and ATP-binding cassette sub-family B member 1 in peripheral blood: A prospective indicator for drug resistance prediction in non-small cell lung cancer. Oncology Letters, 11, 3033-3039. https://doi.org/10.3892/ol.2016.4369
MLA
Kong, Q., Han, Z., Zuo, X., Wei, H., Huang, W."Co-expression of pregnane X receptor and ATP-binding cassette sub-family B member 1 in peripheral blood: A prospective indicator for drug resistance prediction in non-small cell lung cancer". Oncology Letters 11.5 (2016): 3033-3039.
Chicago
Kong, Q., Han, Z., Zuo, X., Wei, H., Huang, W."Co-expression of pregnane X receptor and ATP-binding cassette sub-family B member 1 in peripheral blood: A prospective indicator for drug resistance prediction in non-small cell lung cancer". Oncology Letters 11, no. 5 (2016): 3033-3039. https://doi.org/10.3892/ol.2016.4369
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