Open Access

The proliferative effects of asbestos-exposed peripheral blood mononuclear cells on mesothelial cells

  • Authors:
    • Yuho Maki
    • Yasumitsu Nishimura
    • Shinichi Toyooka
    • Junichi Soh
    • Kazunori Tsukuda
    • Kazuhiko Shien
    • Masashi Furukawa
    • Takayuki Muraoka
    • Tsuyoshi Ueno
    • Norimitsu Tanaka
    • Hiromasa Yamamoto
    • Hiroaki Asano
    • Megumi Maeda
    • Naoko Kumagai‑Takei
    • Suni Lee
    • Hidenori Matsuzaki
    • Takemi Otsuki
    • Shinichiro Miyoshi
  • View Affiliations

  • Published online on: April 5, 2016     https://doi.org/10.3892/ol.2016.4412
  • Pages: 3308-3316
  • Copyright: © Maki et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Malignant mesothelioma (MM) is thought to arise from the direct effect of asbestos on mesothelial cells. However, MM takes a long time to develop following exposure to asbestos, which suggests that the effects of asbestos are complex. The present study examined the effects of asbestos exposure on the cell growth of MeT-5A human mesothelial cells via cytokines produced by immune cells. Peripheral blood mononuclear cells (PBMCs) were stimulated with antibodies against cluster of differentiation (CD)3 and CD28 upon exposure to the asbestos chrysotile A (CA) or crocidolite (CR); the growth of MeT‑5A cells in media supplemented with PBMC culture supernatants was subsequently examined. MeT‑5A cells exhibited an increase in proliferation when grown in supernatant from the 7‑day PBMC culture exposed to CA or CR. Analysis of cytokine production demonstrated increased levels of granulocyte colony‑stimulating factor (G‑CSF), granulocyte-macrophage colony‑stimulating factor (GM‑CSF), interleukin (IL)‑1α, IL‑1β, IL‑3, IL‑5, IL‑13 and IL‑17A in supernatants. Individual administration of these cytokines, excluding G‑CSF and GM‑CSF, led to an increase in cell growth of MeT‑5A, whereas this effect was not observed following the combined administration of these cytokines. The results indicate that cytokines secreted by immune cells upon exposure to asbestos cause an increase in the growth activity of mesothelial cells, suggesting that alterations in the production of cytokines by immune cells may contribute to tumorigenesis in individuals exposed to asbestos.
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May-2016
Volume 11 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Maki Y, Nishimura Y, Toyooka S, Soh J, Tsukuda K, Shien K, Furukawa M, Muraoka T, Ueno T, Tanaka N, Tanaka N, et al: The proliferative effects of asbestos-exposed peripheral blood mononuclear cells on mesothelial cells. Oncol Lett 11: 3308-3316, 2016
APA
Maki, Y., Nishimura, Y., Toyooka, S., Soh, J., Tsukuda, K., Shien, K. ... Miyoshi, S. (2016). The proliferative effects of asbestos-exposed peripheral blood mononuclear cells on mesothelial cells. Oncology Letters, 11, 3308-3316. https://doi.org/10.3892/ol.2016.4412
MLA
Maki, Y., Nishimura, Y., Toyooka, S., Soh, J., Tsukuda, K., Shien, K., Furukawa, M., Muraoka, T., Ueno, T., Tanaka, N., Yamamoto, H., Asano, H., Maeda, M., Kumagai‑Takei, N., Lee, S., Matsuzaki, H., Otsuki, T., Miyoshi, S."The proliferative effects of asbestos-exposed peripheral blood mononuclear cells on mesothelial cells". Oncology Letters 11.5 (2016): 3308-3316.
Chicago
Maki, Y., Nishimura, Y., Toyooka, S., Soh, J., Tsukuda, K., Shien, K., Furukawa, M., Muraoka, T., Ueno, T., Tanaka, N., Yamamoto, H., Asano, H., Maeda, M., Kumagai‑Takei, N., Lee, S., Matsuzaki, H., Otsuki, T., Miyoshi, S."The proliferative effects of asbestos-exposed peripheral blood mononuclear cells on mesothelial cells". Oncology Letters 11, no. 5 (2016): 3308-3316. https://doi.org/10.3892/ol.2016.4412