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Article

Smad4 inhibits cell migration via suppression of JNK activity in human pancreatic carcinoma PANC‑1 cells

  • Authors:
    • Xueying Zhang
    • Junxia Cao
    • Yujun Pei
    • Jiyan Zhang
    • Qingyang Wang
  • View Affiliations / Copyright

    Affiliations: Department of Molecular Immunology, Institute of Basic Medical Sciences, Beijing 100850, P.R. China
  • Pages: 3465-3470
    |
    Published online on: April 7, 2016
       https://doi.org/10.3892/ol.2016.4427
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Abstract

Smad4 is a common Smad and is a key downstream regulator of the transforming growth factor‑β signaling pathway, in which Smad4 often acts as a potent tumor suppressor and functions in a highly context‑dependent manner, particularly in pancreatic cancer. However, little is known regarding whether Smad4 regulates other signaling pathways involved in pancreatic cancer. The present study demonstrated that Smad4 downregulates c‑Jun N‑terminal kinase (JNK) activity using a Smad4 loss‑of‑function or gain‑of‑function analysis. Additionally, stable overexpression of Smad4 clearly affected the migration of human pancreatic epithelioid carcinoma PANC‑1 cells, but did not affect cell growth. In addition, the present study revealed that upregulation of mitogen‑activated protein kinase phosphatase‑1 is required for the reduction of JNK activity by Smad4, leading to a decrease in vascular endothelial growth factor expression and inhibiting cell migration. Overall, the present findings indicate that Smad4 may suppress JNK activation and inhibit the tumor characteristics of pancreatic cancer cells.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang X, Cao J, Pei Y, Zhang J and Wang Q: Smad4 inhibits cell migration via suppression of JNK activity in human pancreatic carcinoma PANC‑1 cells. Oncol Lett 11: 3465-3470, 2016.
APA
Zhang, X., Cao, J., Pei, Y., Zhang, J., & Wang, Q. (2016). Smad4 inhibits cell migration via suppression of JNK activity in human pancreatic carcinoma PANC‑1 cells. Oncology Letters, 11, 3465-3470. https://doi.org/10.3892/ol.2016.4427
MLA
Zhang, X., Cao, J., Pei, Y., Zhang, J., Wang, Q."Smad4 inhibits cell migration via suppression of JNK activity in human pancreatic carcinoma PANC‑1 cells". Oncology Letters 11.5 (2016): 3465-3470.
Chicago
Zhang, X., Cao, J., Pei, Y., Zhang, J., Wang, Q."Smad4 inhibits cell migration via suppression of JNK activity in human pancreatic carcinoma PANC‑1 cells". Oncology Letters 11, no. 5 (2016): 3465-3470. https://doi.org/10.3892/ol.2016.4427
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang X, Cao J, Pei Y, Zhang J and Wang Q: Smad4 inhibits cell migration via suppression of JNK activity in human pancreatic carcinoma PANC‑1 cells. Oncol Lett 11: 3465-3470, 2016.
APA
Zhang, X., Cao, J., Pei, Y., Zhang, J., & Wang, Q. (2016). Smad4 inhibits cell migration via suppression of JNK activity in human pancreatic carcinoma PANC‑1 cells. Oncology Letters, 11, 3465-3470. https://doi.org/10.3892/ol.2016.4427
MLA
Zhang, X., Cao, J., Pei, Y., Zhang, J., Wang, Q."Smad4 inhibits cell migration via suppression of JNK activity in human pancreatic carcinoma PANC‑1 cells". Oncology Letters 11.5 (2016): 3465-3470.
Chicago
Zhang, X., Cao, J., Pei, Y., Zhang, J., Wang, Q."Smad4 inhibits cell migration via suppression of JNK activity in human pancreatic carcinoma PANC‑1 cells". Oncology Letters 11, no. 5 (2016): 3465-3470. https://doi.org/10.3892/ol.2016.4427
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