Cbl‑b promotes cell detachment via ubiquitination of focal adhesion kinase

Introduction

The first step of tumor metastasis is cell detachment from a primary tumor (1). Normally, adhesions between normal epithelial cells and the cellular matrix are strong; cell detachment may be achieved via downregulation of cell adhesion molecules (2). It has been reported that loss of E-cadherin results in the detachment of adjacent cells (3,4). Focal adhesion kinase (FAK) is a central regulator of focal adhesion, promoting cell adhesion and metastasis (5). Previous studies have reported that simultaneous inhibition of FAK promotes the detachment of colon cancer cells and fibroblasts (6,7). However, Wade et al (8) reported that FAK tyrosine phosphorylation was present in detached fibroblast cells. Therefore, the involvement of FAK in cell detachment remains controversial.

Casitas B-lineage lymphoma-b (Cbl-b), a RING finger E3 ubiquitin protein ligase, is composed of a tyrosine kinase binding domain, an ubiquitin-associated domain, RING finger domains and several tyrosine phosphorylation sites. It has been hypothesized that Cbl-b exhibits a crucial function as a ubiquitin ligase and multifunctional adaptor molecule (9). A previous study demonstrated that Cbl-b is involved in the regulation of the cell cytoskeleton and adhesion (10). Furthermore, it has been reported that Cbl-b reduces cell adhesion via negative regulation of integrin and receptor-mediated signaling (11). By contrast, a previous study reported that Cbl-b enhances cell-to-cell adherens junctions and cell adhesion (12). Schmidt and Dikic (13) revealed that c-Cbl, a homolog of Cbl-b, associates with FAK and affects cellular attachment to the extracellular matrix (ECM). In addition, Rafiq et al (14) demonstrated that c-Cbl interacts with FAK, resulting in enhanced c-Cbl-mediated FAK ubiquitination and subsequent downregulation of myocyte survival signaling. However, whether Cbl-b targets FAK for degradation and its involvement in the process of cell detachment requires further investigation.

The aim of the present study was to evaluate the effect of FAK and its function in the process of tumor cell detachment. The present study demonstrated that FAK suppressed trypsin-induced cell detachment, and lysosome inhibitor NH4Cl suppressed cell detachment through mono-ubiquitination of FAK, while Cbl-b promoted cell detachment through ubiquitination of FAK. These results provide novel insights into the role of Cbl-b and FAK in cell detachment.

Materials and methods

Reagents and antibodies

Antibodies against Cbl-b (mouse monoclonal catalog no., sc-8006; dilution, 1:250) and actin (rabbit polyclonal; catalog no., H-196; dilution, 1:1,000) were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Antibodies against Akt (rabbit polyclonal; catalog no., 4691; dilution, 1:1,000), phosphorylated (p)-Akt (rabbit polyclonal; catalog no., 4060; dilution, 1:500), FAK (rabbit polyclonal; catalog no., 3285; dilution, 1:500), anti-p-FAK (rabbit polyclonal; catalog no., 8556; dilution, 1:500) and ubiquitin (rabbit polyclonal; catalog no., 3936; dilution, 1:500) were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA). Dimethyl sulfoxide was obtained from Sigma-Aldrich (St. Louis, MO, USA).

Cell culture

The human gastric adenocarcinoma MGC803, lung adenocarcinoma A549, colon carcinoma RKO, breast adenocarcinoma MCF-7, breast carcinoma MDA-MB-231 and gastric epithelial cell GES-1 cell lines were obtained from the Academy of Military Medical Sciences (Beijing, China). The cells were cultured in RPMI-1640 medium or L-15 medium containing 10% fetal calf serum (all Gibco®; Thermo Fisher Scientific, Inc., Waltham, MA, USA) at 37°C in a humidified incubator with an atmosphere of 5% CO2.

MTT assay

The percentage of detached cells was analyzed using MTT assay. The cells (2×105 cells/well) were treated with 2.5 mg/l trypsin for various times (0, 1, 3 and 5 min) and washed four times with ice-cold phosphate-buffered saline. The MTT assay was performed as described in a previous study by the present authors (15).

Western blot analysis

The cells (2×105 cells/well) were seeded in 6-well plates and incubated overnight. The cells were treated with 2.5 mg/l trypsin for various times (0, 1, 3 and 5 min). The medium was removed and the cells were added to a 1.5 ml Eppendorf tube followed by transient centrifugalization. Western blot analysis was performed as described in a previous study (15). Western blot images were analyzed using National Institutes of Health image software (rsb.info.nih.gov/nih-image/) for further statistical analysis.

Immunoprecipitation

The antibodies against FAK and Cbl-b, protein G-agarose beads (Cell Signaling Technology, Inc.) and cell lysate were incubated overnight at 4°C. Subsequently, immunoprecipitates were washed four times with lysis buffer. Immunoprecipitation was performed as described in a previous study (16).

Plasmid construction and transfection

Plasmid construction was performed as described in a previous study (15). Briefly, MGC803 and MDA-MB-231 cells were transfected with short hairpin (sh) RNA targeting Cbl-b using Lipofectamine 2000 reagent (Invitrogen™; Thermo Fisher Scientific, Inc), according to the manufacturer's protocol. One set of synthetic oligonucleotides involved the sense and antisense target sequences of human Cbl-b: Sense, 5′-GGATCCCGGATGTGTTTGGGACTAATTTGATATCCGATTAGTCCCAAACACATCCTTTTTTCCAAAAGCTT-3′ and antisense, 5′-AAGCTTTTGGAAAAAAGGATGTGTTTGGGACTAATCGGATATCAAATTAGTCCCAAACACATCCGGGATCC-3′ were annealed and ligated into the BamHI/HindIII-cleaved backbone of pRNA-U6.1/Neo (Genescript, Piscataway, NJ, USA). Stably transfected cell lines were selected according to the methods described in a previous study (17). The cDNA of Flag-tagged ubiquitin (Flag-Ub) was provided by Dr Kiyonao Sada (Division of Genome Science and Microbiology, University of Fukui, Fukui, Japan). All cDNAs were then subcloned into the pSVL expression vector (GE Healthcare, Piscataway, NJ, USA). MGC803/NC and MGC803/shCbl-b, MDA-MB-231/NC and MDA-MB-231/shCbl-b cells transfected with shRNA-Cbl-b were used for the following experiments.

Small interfering (si)RNA transfections

FAK siRNA was obtained from Shanghai GeneChem Co. Ltd., (Shanghai, China). MGC803 cells were transfected with siRNAs using Lipofectamine 2000, according to the manufacturer's protocol. FAK siRNA sequences were synthesized as follows: FAK siRNA sequences were synthesized as follows: siFAK-1, CAGGUGAAGAGCGAUUAU Att; siFAK-2, CUCCAGUCUACAGAUUUGAtt; siFAK-3, CCCAGGUUUACUGAACUUAtt. After 48 h of transient transfection, the cells were analyzed by western blotting to determine the effect of FAK siRNA on FAK expression.

Statistical analysis

All experiments were performed in triplicate. Data were expressed as the mean ± standard deviation. Statistical significance was determined using the Student's t-test. Statistical analysis was performed using SPSS version 18.0 software (SPSS, Inc., Chicago, IL, USA). P<0.05 was considered to indicate a statistically significant difference.

Results

Decreased FAK expression is associated with trypsin-induced cell detachment

To investigate the effect of FAK in cell detachment, the human gastric cancer MGC803 cell line was treated with trypsin for 0, 1, 3 and 5 min. The percentage of detached cells was significantly increased in a time-dependent manner (Fig. 1A; P<0.05). Furthermore, western blot analysis revealed that FAK was gradually reduced in a time-dependent manner (Fig. 1B). Similar results were observed in human breast cancer MCF-7, lung cancer A549, colon cancer RKO and gastric epithelial GES-1 cells (Fig. 1C; P<0.05). These results indicate that FAK is downregulated in the process of cell detachment.

Figure 1. FAK expression was decreased in human gastric adenocarcinoma MGC803 cells following treatment with trypsin for 1, 3 and 5 min. (A) The percentage of detached cells was quantified using MTT assay, which revealed that the percentage of detached cells increased in a time-dependent manner following trypsin treatment. FAK expression was analyzed in (B) MGC803 and (C) human breast adenocarcinoma MCF-7, lung adenocarcinoma A549, colon carcinoma RKO and gastric epithelial GES-1 cell lines by western blotting. FAK expression was decreased in a time-dependent manner in all cell lines. Results are representative of three independent experiments. Data were expressed as the mean ± standard deviation. *P<0.05 vs. 0 min. FAK, focal adhesion kinase; p, phosphorylated.

FAK inhibits trypsin-induced cell detachment

To further clarify the function of FAK in the process of cell detachment, MGC803 cells were transfected with FAK-specific siRNAs for 48 h. Knockdown of FAK expression was detected by western blotting (Fig. 2A). siRNA 3 was selected for further analysis, which revealed that knockdown of FAK significantly increased cell detachment compared with controls in a time-dependent manner (P<0.05; Fig. 2B). In addition, p-Akt levels were decreased in the siRNA/FAK group (Fig. 2C). These data indicate that FAK inhibits cell detachment in cancer cells and human gastric epithelial cells.

Figure 2. Knockdown of FAK increased cell detachment. Human gastric adenocarcinoma MGC803 cells were transiently transfected with FAK-specific siRNAs for 48 h. (A) Expression of FAK was analyzed by western blotting. (B) The percentage of detached cells in siRNA-transfected MGC803 cells was quantified by MTT assay, which revealed that transfection with siRNA FAK 3 significantly increased cell detachment. (C) Expression of FAK and Akt were analyzed by western blotting. Results are representative of three independent experiments. Data were expressed as the mean ± standard deviation. *P<0.05 vs. siRNA control. FAK, focal adhesion kinase; siRNA, small interfering RNA; p, phosphorylated.

Ubiquitin-lysosome degradation mediates trypsin-induced degradation of FAK

A previous study demonstrated that ubiquitin-dependent protein degradation is critical in the regulation of FAK (14). However, whether ubiquitination degradation mediates trypsin-induced degradation of FAK is currently unknown. In the present study, MGC803 cells were transfected with plasmids expressing Flag-Ub. Mono-ubiquitination of FAK was increased in a time-dependent manner following trypsin treatment of the transfected cells (Fig. 3A). In the NH4Cl-pretreated group, FAK degradation was suppressed compared with the control (Fig. 3B). However, similar results were not observed in cells pretreated with the proteasome inhibitor PS-341 (Fig. 3C). To further investigate whether the degradation of FAK occurs via the ubiquitin-lysosome system, MGC803 cells were transfected with plasmid expressing Flag-Ub for 48 h, in the absence or presence of 50 nM NH4Cl for 12 h. Mono-ubiquitination of FAK was enhanced in the NH4Cl pretreated group (Fig. 3D). These results indicate that the ubiquitin-lysosome system mediates trypsin-induced degradation of FAK.

Figure 3. Trypsin induced mono-ubiquitination and degradation of FAK via ubiquitin-lysosome system. (A) Human gastric adenocarcinoma MGC803 cells were transfected with plasmids expressing Flag-Ub and treated with trypsin for 1 and 5 min and the expression of ubiquitinated FAK was analyzed. (B and C) MGC803 cells were pretreated with (B) NH4Cl or (C) PS-341 and FAK levels were analyzed by western blotting. (D) MGC803 cells were transfected with plasmids expressing Flag-Ub for 48 h then treated with 50 nM NH4Cl for 12 h. Mono-ubiquitination of FAK was analyzed by western blotting. Results are representative of three independent experiments. FAK, focal adhesion kinase; p, phosphorylated; Ub, ubiquitin; Flag-Ub, Flag-ubiquitin.

Cbl-b accelerates trypsin-induced cell detachment

A previous study demonstrated that the E3 ubiquitin protein ligase c-Cbl is involved in the ubiquitination of FAK (14). Therefore, the involvement of Cbl-b in ubiquitination and degradation of FAK requires further investigation. In the present study, MGC803 cells were treated with trypsin for 1 and 5 min and the level of Cbl-b protein was analyzed. The expression of Cbl-b increased in a time-dependent manner (Fig. 4A). Furthermore, Cbl-b interacted with FAK in the absence and presence of trypsin treatment, as demonstrated by co-immunoprecipitation (Fig. 4B). In order to further investigate the function of Cbl-b in cell detachment, previously established shRNA plasmids targeting Cbl-b, which were stably transfected into MGC803 cells (16), were used for further experiments. The shRNA plasmids targeting Cbl-b were also stably transfected into MDA-MB-231 cells and Cbl-b expression was evaluated by western blotting (Fig. 4C). Knockdown of Cbl-b increased the expression of FAK (Fig. 4D). These results indicate that Cbl-b accelerates trypsin-induced cell detachment via degradation of FAK.

Figure 4. Cbl-b promoted trypsin-induced degradation of FAK. (A) Human gastric adenocarcinoma MGC803 cells were treated with trypsin for 1 and 5 min. Cbl-b expression was analyzed by western blotting. (B) MGC803 cells were treated with trypsin for 5 min. Co-immunoprecipitation revealed that FAK protein and Cbl-b interacted with each other. (C) Western blotting analysis revealed a knockdown effect of Cbl-b in MGC803 and human breast carcinoma MDA-MB-231 cells transfected with shRNA-Cbl-b. (D) MGC803 and MDA-MB-231 NC cells and Cbl-b knockdown cells were treated with trypsin for 1 and 5 min. FAK expression was analyzed by western blotting. The results are representative of three independent experiments. Cbl-b, Casitas B-lineage lymphoma-b; FAK, focal adhesion kinase; p, phosphorylated; NC, nonsilencing control; shRNA, short hairpin RNA.

Discussion

It has been reported that FAK not only promotes cell adhesion, but is also involved in cell detachment (6–8,17). Kim et al (18) reported that okadaic acid induces cell detachment, which is accompanied by FAK dephosphorylation. Zouq et al (7) reported that fibroblasts and epithelial cells exhibit rapid dephosphorylation of FAK in response to detachment from the ECM. Conversely, overexpression of the FAK N-terminus induces rounding, detachment and apoptosis in breast carcinoma cells (17). Thus, the effect of FAK in cell detachment remains controversial. In the present study, expression and phosphorylation of FAK were decreased following trypsin-induced detachment in cancer cell lines. Knockdown of FAK further enhanced trypsin-induced cell detachment. These data indicate that FAK inhibits trypsin-induced cell detachment.

The ubiquitin-dependent protein degradation pathway is important for the regulation of FAK (14,19). A previous study has demonstrated that Cat.G, a neutrophil-derived serine protease, induces ubiquitin-proteasome-dependent degradation of FAK (14). Inhibition of proteasome activity markedly attenuates FAK degradation in T-, myofibril and ovarian carcinoma cells (14,20,21). In the present study, trypsin induced the mono-ubiquitination of FAK in a time-dependent manner. Additionally, the downregulation of FAK was suppressed by pretreatment with the lysosome inhibitor NH4Cl. However, similar results were not observed following pretreatment with the proteasome inhibitor PS-341. Furthermore, ubiquitination of FAK was increased in cells pretreated with NH4Cl. These results indicate that the ubiquitin-lysosome system mediates trypsin-induced degradation of FAK.

Results from previous studies have revealed that MG53, an E3 ubiquitin ligase, mediates FAK ubiquitination during skeletal myogenesis (21), and that c-Cbl is involved in FAK degradation thereby suppressing cell adhesion and myocyte survival (14). Cbl-b, a negative regulator of non-receptor tyrosine kinases, may cause FAK ubiquitination and degradation. In the present study, Cbl-b interacted with FAK in the absence and presence of trypsin. Knockdown of Cbl-b increased FAK expression and decreased trypsin-induced degradation of FAK. These findings further indicate that Cbl-b accelerates cell detachment via mono-ubiquitination of FAK.

In conclusion, the results of the present study indicate that cell detachment is mediated via ubiquitin-lysosome degradation of FAK. Furthermore, the E3 ubiquitin ligase Cbl-b contributes to cell detachment by facilitating FAK degradation. These findings provide novel insights with regard to the functions of Cbl-b and FAK in the process of cell detachment.

Acknowledgements

This study was supported by the Chinese National Foundation of National Sciences (grant nos. 81172369, 81302128, 81472193 and 81372547), the General Project of Liaoning Province Department of Education (grant no. L2014296), the National Science and Technology Major Project (grant no. 2013ZX09303002) and the Science and Technology Plan Project of Liaoning Province (grant no. 2014225013).

References