Expression of microRNA-30a-5p in drug-resistant and drug-sensitive ovarian cancer cell lines

  • Authors:
    • Jin Liu
    • Xiaohua Wu
    • Hongmei Liu
    • Yijuan Liang
    • Xinping Gao
    • Zhihui Cai
    • Weiming Wang
    • Hui Zhang
  • View Affiliations

  • Published online on: July 8, 2016     https://doi.org/10.3892/ol.2016.4831
  • Pages: 2065-2070
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Abstract

The present study aimed to explore the expression of microRNA (miRNA or miR) in drug‑resistant and drug‑sensitive ovarian cancer cell lines, and to seek the potential therapeutic target of ovarian cancer drug‑resistant mechanism in order to improve drug resistance by altering miRNA levels. The drug‑resistant characteristics of SKOV3/DDP, SKOV3, COC1/DDP and COC1 cell lines were studied. The miRNAs that were differentially expressed between cisplatin‑resistant cells and its parental cells in ovarian cancer were screened with a miRNA chip. The effect of miRNAs was detected, and their drug‑resistant mechanism was investigated by transfection and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide methods. Among the expression screening of miRNAs, 41 mRNAs, including Homo sapiens (hsa)-miR-30a-5p and hsa-miR-34c-5p, were highly expressed in the drug‑resistant cells, whereas 44 miRNAs, including hsa-miR-96-5p and hsa‑miR‑200c‑3p, were lowly expressed. The expression levels of hsa‑miR‑30a‑5p in two types of ovarian cancer chemotherapy‑resistant cell lines were significantly higher than those in chemotherapy‑sensitive cell lines, which was associated with ovarian cancer chemotherapy resistance. In conclusion, high expression of miRNA‑30a‑5p was able to promote cell growth and colony forming ability, and enhance cell migration and invasion. Thus, miRNA‑30a‑5p is expected to become a meaningful novel target for ovarian cancer resistant treatment.
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September-2016
Volume 12 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Liu J, Wu X, Liu H, Liang Y, Gao X, Cai Z, Wang W and Zhang H: Expression of microRNA-30a-5p in drug-resistant and drug-sensitive ovarian cancer cell lines. Oncol Lett 12: 2065-2070, 2016
APA
Liu, J., Wu, X., Liu, H., Liang, Y., Gao, X., Cai, Z. ... Zhang, H. (2016). Expression of microRNA-30a-5p in drug-resistant and drug-sensitive ovarian cancer cell lines. Oncology Letters, 12, 2065-2070. https://doi.org/10.3892/ol.2016.4831
MLA
Liu, J., Wu, X., Liu, H., Liang, Y., Gao, X., Cai, Z., Wang, W., Zhang, H."Expression of microRNA-30a-5p in drug-resistant and drug-sensitive ovarian cancer cell lines". Oncology Letters 12.3 (2016): 2065-2070.
Chicago
Liu, J., Wu, X., Liu, H., Liang, Y., Gao, X., Cai, Z., Wang, W., Zhang, H."Expression of microRNA-30a-5p in drug-resistant and drug-sensitive ovarian cancer cell lines". Oncology Letters 12, no. 3 (2016): 2065-2070. https://doi.org/10.3892/ol.2016.4831