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Article

Expression of microRNA-30a-5p in drug-resistant and drug-sensitive ovarian cancer cell lines

  • Authors:
    • Jin Liu
    • Xiaohua Wu
    • Hongmei Liu
    • Yijuan Liang
    • Xinping Gao
    • Zhihui Cai
    • Weiming Wang
    • Hui Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China, Department of Molecular Medicine, Hebei University, Baoding, Hebei 071000, P.R. China, Department of Gynecology, Affiliated Hospital of Hebei University, Baoding, Hebei 071000, P.R. China
  • Pages: 2065-2070
    |
    Published online on: July 8, 2016
       https://doi.org/10.3892/ol.2016.4831
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Abstract

The present study aimed to explore the expression of microRNA (miRNA or miR) in drug‑resistant and drug‑sensitive ovarian cancer cell lines, and to seek the potential therapeutic target of ovarian cancer drug‑resistant mechanism in order to improve drug resistance by altering miRNA levels. The drug‑resistant characteristics of SKOV3/DDP, SKOV3, COC1/DDP and COC1 cell lines were studied. The miRNAs that were differentially expressed between cisplatin‑resistant cells and its parental cells in ovarian cancer were screened with a miRNA chip. The effect of miRNAs was detected, and their drug‑resistant mechanism was investigated by transfection and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide methods. Among the expression screening of miRNAs, 41 mRNAs, including Homo sapiens (hsa)-miR-30a-5p and hsa-miR-34c-5p, were highly expressed in the drug‑resistant cells, whereas 44 miRNAs, including hsa-miR-96-5p and hsa‑miR‑200c‑3p, were lowly expressed. The expression levels of hsa‑miR‑30a‑5p in two types of ovarian cancer chemotherapy‑resistant cell lines were significantly higher than those in chemotherapy‑sensitive cell lines, which was associated with ovarian cancer chemotherapy resistance. In conclusion, high expression of miRNA‑30a‑5p was able to promote cell growth and colony forming ability, and enhance cell migration and invasion. Thus, miRNA‑30a‑5p is expected to become a meaningful novel target for ovarian cancer resistant treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Liu J, Wu X, Liu H, Liang Y, Gao X, Cai Z, Wang W and Zhang H: Expression of microRNA-30a-5p in drug-resistant and drug-sensitive ovarian cancer cell lines. Oncol Lett 12: 2065-2070, 2016.
APA
Liu, J., Wu, X., Liu, H., Liang, Y., Gao, X., Cai, Z. ... Zhang, H. (2016). Expression of microRNA-30a-5p in drug-resistant and drug-sensitive ovarian cancer cell lines. Oncology Letters, 12, 2065-2070. https://doi.org/10.3892/ol.2016.4831
MLA
Liu, J., Wu, X., Liu, H., Liang, Y., Gao, X., Cai, Z., Wang, W., Zhang, H."Expression of microRNA-30a-5p in drug-resistant and drug-sensitive ovarian cancer cell lines". Oncology Letters 12.3 (2016): 2065-2070.
Chicago
Liu, J., Wu, X., Liu, H., Liang, Y., Gao, X., Cai, Z., Wang, W., Zhang, H."Expression of microRNA-30a-5p in drug-resistant and drug-sensitive ovarian cancer cell lines". Oncology Letters 12, no. 3 (2016): 2065-2070. https://doi.org/10.3892/ol.2016.4831
Copy and paste a formatted citation
x
Spandidos Publications style
Liu J, Wu X, Liu H, Liang Y, Gao X, Cai Z, Wang W and Zhang H: Expression of microRNA-30a-5p in drug-resistant and drug-sensitive ovarian cancer cell lines. Oncol Lett 12: 2065-2070, 2016.
APA
Liu, J., Wu, X., Liu, H., Liang, Y., Gao, X., Cai, Z. ... Zhang, H. (2016). Expression of microRNA-30a-5p in drug-resistant and drug-sensitive ovarian cancer cell lines. Oncology Letters, 12, 2065-2070. https://doi.org/10.3892/ol.2016.4831
MLA
Liu, J., Wu, X., Liu, H., Liang, Y., Gao, X., Cai, Z., Wang, W., Zhang, H."Expression of microRNA-30a-5p in drug-resistant and drug-sensitive ovarian cancer cell lines". Oncology Letters 12.3 (2016): 2065-2070.
Chicago
Liu, J., Wu, X., Liu, H., Liang, Y., Gao, X., Cai, Z., Wang, W., Zhang, H."Expression of microRNA-30a-5p in drug-resistant and drug-sensitive ovarian cancer cell lines". Oncology Letters 12, no. 3 (2016): 2065-2070. https://doi.org/10.3892/ol.2016.4831
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