PRL-3 promotes cell adhesion by interacting with JAM2 in colon cancer

Lian,Shenyi; Meng,Lin; Xing,Xiaofang; Yang,Yongyong; Qu,Like; Shou,Chengchao

doi:10.3892/ol.2016.4836

PRL-3 promotes cell adhesion by interacting with JAM2 in colon cancer

Authors:
- Shenyi Lian
- Lin Meng
- Xiaofang Xing
- Yongyong Yang
- Like Qu
- Chengchao Shou
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Affiliations: Department of Biochemistry and Molecular Biology, Key Laboratory of Carcinogenesis and Translational Research, Peking University Cancer Hospital & Institute, Beijing 100142, P.R. China, Department of Gastrointestinal Translational Research, Key Laboratory of Carcinogenesis and Translational Research, Peking University Cancer Hospital & Institute, Beijing 100142, P.R. China
Published online on: July 11, 2016 https://doi.org/10.3892/ol.2016.4836
Pages: 1661-1666
Copyright: © Lian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Phosphatase of regenerating liver-3 (PRL-3), also termed PTP4A3, is a metastasis-related protein tyrosine phosphatase. Its expression levels are significantly correlated with the progression and survival of a wide range of malignant tumors. However, the mechanism by which PRL‑3 promotes tumor invasion and metastasis is not clear. In the present study, the functions of PRL‑3 were systemically analyzed in the key events of metastasis including, motility and adhesion. A cell wounding assay, cell spread assay and cell‑matrix adhesion assay were carried out to analyze the cell movement and cell adhesion ability of colon cancer, immunoprecipitation and immunofluorescence assay was confirmed the interaction of PRL‑3 and JAM2. It was demonstrated that PRL‑3 promoted the motility of Flp‑In‑293 and LoVo colon cancer cells and increased the distribution of cell skeleton proteins on the cell protrusions. In addition, stably expressing PRL‑3 reduced the spreading speed of colon cancer cells and cell adhesion on uncoated, fibronectin-coated and collagen Ⅰ‑coated plates. Mechanistically, junction adhesion molecular 2 (JAM2) was identified as a novel interacting protein of PRL‑3. The findings of the present study revealed the roles of PRL‑3 in cancer cell motility and adhesion process, and provided information on the possibility of PRL-3 increase cell-cell adhesion by associating with JAM2.

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