Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
September-2016 Volume 12 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September-2016 Volume 12 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

PRL-3 promotes cell adhesion by interacting with JAM2 in colon cancer

  • Authors:
    • Shenyi Lian
    • Lin Meng
    • Xiaofang Xing
    • Yongyong Yang
    • Like Qu
    • Chengchao Shou
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry and Molecular Biology, Key Laboratory of Carcinogenesis and Translational Research, Peking University Cancer Hospital & Institute, Beijing 100142, P.R. China, Department of Gastrointestinal Translational Research, Key Laboratory of Carcinogenesis and Translational Research, Peking University Cancer Hospital & Institute, Beijing 100142, P.R. China
    Copyright: © Lian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1661-1666
    |
    Published online on: July 11, 2016
       https://doi.org/10.3892/ol.2016.4836
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Phosphatase of regenerating liver-3 (PRL-3), also termed PTP4A3, is a metastasis-related protein tyrosine phosphatase. Its expression levels are significantly correlated with the progression and survival of a wide range of malignant tumors. However, the mechanism by which PRL‑3 promotes tumor invasion and metastasis is not clear. In the present study, the functions of PRL‑3 were systemically analyzed in the key events of metastasis including, motility and adhesion. A cell wounding assay, cell spread assay and cell‑matrix adhesion assay were carried out to analyze the cell movement and cell adhesion ability of colon cancer, immunoprecipitation and immunofluorescence assay was confirmed the interaction of PRL‑3 and JAM2. It was demonstrated that PRL‑3 promoted the motility of Flp‑In‑293 and LoVo colon cancer cells and increased the distribution of cell skeleton proteins on the cell protrusions. In addition, stably expressing PRL‑3 reduced the spreading speed of colon cancer cells and cell adhesion on uncoated, fibronectin-coated and collagen Ⅰ‑coated plates. Mechanistically, junction adhesion molecular 2 (JAM2) was identified as a novel interacting protein of PRL‑3. The findings of the present study revealed the roles of PRL‑3 in cancer cell motility and adhesion process, and provided information on the possibility of PRL-3 increase cell-cell adhesion by associating with JAM2.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Wan L, Pantel K and Kang Y: Tumor metastasis: Moving new biological insights into the clinic. Nat Med. 19:1450–1464. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Maryáš J, Faktor J, Dvořáková M, Struhárová I, Grell P and Bouchal P: Proteomics in investigation of cancer metastasis: Functional and clinical consequences and methodological challenges. Proteomics. 14:426–440. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Al-Aidaroos AQ and Zeng Q: PRL-3 phosphatase and cancer metastasis. J Cell Biochem. 111:1087–1098. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Bessette DC, Qiu D and Pallen CJ: PRL PTPs: Mediators and markers of cancer progression. Cancer Metastasis Rev. 27:231–252. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Saha S, Bardelli A, Buckhaults P, Velculescu VE, Rago C, St Croix B, Romans KE, Choti MA, Lengauer C, Kinzler KW and Vogelstein B: A phosphatase associated with metastasis of colorectal cancer. Science. 294:1343–1346. 2001. View Article : Google Scholar : PubMed/NCBI

6 

Xing X, Peng L, Qu L, Ren T, Dong B, Su X and Shou C: Prognostic value of PRL-3 overexpression in early stages of colonic cancer. Histopathology. 54:309–318. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Peng L, Ning J, Meng L and Shou C: The association of the expression level of protein tyrosine phosphatase PRL-3 protein with liver metastasis and prognosis of patients with colorectal cancer. J Cancer Res Clin Oncol. 130:521–526. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Li Z, Zhan W, Wang Z, Zhu B, He Y, Peng J, Cai S and Ma J: Inhibition of PRL-3 gene expression in gastric cancer cell line SGC7901 via microRNA suppressed reduces peritoneal metastasis. Biochem Biophys Res Commun. 348:229–237. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Fiordalisi JJ, Keller PJ and Cox AD: PRL tyrosine phosphatases regulate rho family GTPases to promote invasion and motility. Cancer Res. 66:3153–3161. 2006. View Article : Google Scholar : PubMed/NCBI

10 

Peng L, Xing X, Li W, Qu L, Meng L, Lian S, Jiang B, Wu J and Shou C: PRL-3 promotes the motility, invasion, and metastasis of LoVo colon cancer cells through PRL-3-integrin beta1-ERK1/2 and-MMP2 signaling. Mol Cancer. 8:1102009. View Article : Google Scholar : PubMed/NCBI

11 

Liang F, Luo Y, Dong Y, Walls CD, Liang J, Jiang HY, Sanford JR, Wek RC and Zhang ZY: Translational control of C-terminal Src kinase (Csk) expression by PRL3 phosphatase. J Biol Chem. 283:10339–10346. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Wang H, Quah SY, Dong JM, Manser E, Tang JP and Zeng Q: PRL3 down regulates PTEN expression and signals through PI3K promote epithelial-mesenchymal transition. Cancer Res. 67:2922–2926. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Bazzoni G: The JAM family of junctional adhesion molecules. Curr Opin Cell Biol. 15:525–530. 2003. View Article : Google Scholar : PubMed/NCBI

14 

Bradfield PF, Scheiermann C, Nourshargh S, Ody C, Luscinskas FW, Rainger GE, Nash GB, Miljkovic-Licina M, Aurrand-Lions M and Imhof BA: JAM-C regulates unidirectional monocyte transendothelial migration in inflammation. Blood. 110:2545–2555. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Arcangeli ML, Frontera V, Bardin F, Obrados E, Adams S, Chabannon C, Schiff C, Mancini SJ, Adams RH and Aurrand-Lions M: JAM-B regulates maintenance of hematopoietic stem cells in the bone marrow. Blood. 118:4609–4619. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Doñate C, Ody C, McKee T, Ruault-Jungblut S, Fischer N, Ropraz P, Imhof BA and Matthes T: Homing of human B cells to lymphoid organs and B-cell lymphoma engraftment are controlled by cell adhesion molecule JAM-C. Cancer Res. 73:640–651. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Lamagna C, Meda P, Mandicourt G, Brown J, Gilbert RJ, Jones EY, Kiefer F, Ruga P, Imhof BA and Aurrand-Lions M: Dual interaction of JAM-C with JAM-B and alpha(M)beta2 integrin: Function in junctional complexes and leukocyte adhesion. Mol Biol Cell. 16:4992–5003. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Sakaguchi T, Nishimoto M, Miyagi S, Iwama A, Morita Y, Iwamori N, Nakauchi H, Kiyonari H, Muramatsu M and Okuda A: Putative ‘stemnessʼ gene jam-B is not required for maintenance of stem cell state in embryonic, neural, or hematopoietic stem cells. Mol Cell Biol. 26:6557–6570. 2006. View Article : Google Scholar : PubMed/NCBI

19 

Arcangeli ML, Bardin F, Frontera V, Bidaut G, Obrados E, Adams RH, Chabannon C and Aurrand-Lions M: Function of Jam-B/Jam-C interaction in homing and mobilization of human and mouse hematopoietic stem and progenitor cells. Stem Cells. 32:1043–1054. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Morgan C, Jenkins SA, Kynaston HG and Doak SH: The role of adhesion molecules as biomarkers for the aggressive prostate cancer phenotype. PLoS One. 8:e816662013. View Article : Google Scholar : PubMed/NCBI

21 

Peng L, Li Y, Meng L and Shou C: Preparation and characterization of monoclonal antibody against protein tyrosine phosphatase PRL-3. Hybrid Hybridomics. 23:23–27. 2004. View Article : Google Scholar : PubMed/NCBI

22 

Fife CM, McCarroll JA and Kavallaris M: Movers and shakers: cell cytoskeleton in cancer metastasis. Br J Pharmacol. 171:5507–5523. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Basak S, Jacobs SB, Krieg AJ, Pathak N, Zeng Q, Kaldis P, Giaccia AJ and Attardi LD: The metastasis-associated gene Prl-3 is a p53 target involved in cell-cycle regulation. Mol Cell. 30:303–314. 2008. View Article : Google Scholar : PubMed/NCBI

24 

Rios P, Li X and Köhn M: Molecular mechanisms of the PRL phosphatases. FEBS J. 280:505–524. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Al-Aidaroos AQ, Yuen HF, Guo K, Zhang SD, Chung TH, Chng WJ and Zeng Q: Metastasis-associated PRL-3 induces EGFR activation and addiction in cancer cells. J Clin Invest. 123:3459–3471. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Quail DF and Joyce JA: Microenvironmental regulation of tumor progression and metastasis. Nat Med. 11:1423–1437. 2013. View Article : Google Scholar

27 

Huang YH, Al-Aidaroos AQ, Yuen HF, Zhang SD, Shen HM, Rozycka E, McCrudden CM, Tergaonkar V, Gupta A, Lin YB, et al: A role of autophagy in PTP4A3-driven cancer progression. Autophagy. 10:1787–1800. 2014. View Article : Google Scholar : PubMed/NCBI

28 

Zhang J, Xiao Z, Lai D, Sun J, He C, Chu Z, Ye H, Chen S and Wang J: miR-21, miR-17 and miR-19a induced by phosphatase of regenerating liver-3 promote the proliferation and metastasis of colon cancer. Br J Cancer. 107:352–359. 2012. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Lian S, Meng L, Xing X, Yang Y, Qu L and Shou C: PRL-3 promotes cell adhesion by interacting with JAM2 in colon cancer. Oncol Lett 12: 1661-1666, 2016.
APA
Lian, S., Meng, L., Xing, X., Yang, Y., Qu, L., & Shou, C. (2016). PRL-3 promotes cell adhesion by interacting with JAM2 in colon cancer. Oncology Letters, 12, 1661-1666. https://doi.org/10.3892/ol.2016.4836
MLA
Lian, S., Meng, L., Xing, X., Yang, Y., Qu, L., Shou, C."PRL-3 promotes cell adhesion by interacting with JAM2 in colon cancer". Oncology Letters 12.3 (2016): 1661-1666.
Chicago
Lian, S., Meng, L., Xing, X., Yang, Y., Qu, L., Shou, C."PRL-3 promotes cell adhesion by interacting with JAM2 in colon cancer". Oncology Letters 12, no. 3 (2016): 1661-1666. https://doi.org/10.3892/ol.2016.4836
Copy and paste a formatted citation
x
Spandidos Publications style
Lian S, Meng L, Xing X, Yang Y, Qu L and Shou C: PRL-3 promotes cell adhesion by interacting with JAM2 in colon cancer. Oncol Lett 12: 1661-1666, 2016.
APA
Lian, S., Meng, L., Xing, X., Yang, Y., Qu, L., & Shou, C. (2016). PRL-3 promotes cell adhesion by interacting with JAM2 in colon cancer. Oncology Letters, 12, 1661-1666. https://doi.org/10.3892/ol.2016.4836
MLA
Lian, S., Meng, L., Xing, X., Yang, Y., Qu, L., Shou, C."PRL-3 promotes cell adhesion by interacting with JAM2 in colon cancer". Oncology Letters 12.3 (2016): 1661-1666.
Chicago
Lian, S., Meng, L., Xing, X., Yang, Y., Qu, L., Shou, C."PRL-3 promotes cell adhesion by interacting with JAM2 in colon cancer". Oncology Letters 12, no. 3 (2016): 1661-1666. https://doi.org/10.3892/ol.2016.4836
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team