Targeted silencing of CXCR4 inhibits epithelial-mesenchymal transition in oral squamous cell carcinoma

  • Authors:
    • Yuansheng Duan
    • Shu Zhang
    • Longlong Wang
    • Xuan Zhou
    • Qinghua He
    • Su Liu
    • Kai Yue
    • Xudong Wang
  • View Affiliations

  • Published online on: July 11, 2016     https://doi.org/10.3892/ol.2016.4838
  • Pages: 2055-2061
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Abstract

Aberrant overexpression of C-X-C chemokine receptor type 4 (CXCR4) is a critical event during tumor metastasis. It has been previously reported that the expression of CXCR4 is linked with epithelial-mesenchymal transition (EMT) in oral squamous cell carcinoma (OSCC) tissues derived from patients. The present study addresses the role of CXCR4 in EMT in tongue squamous cell carcinoma (TSCCA) cells in vitro and in xenograft models. Small interfering (si) RNA sequences targeting the CXCR4 gene were transfected into TSCCA cells. Cell migration, invasion, apoptosis and EMT markers were determined in TSCCA cells using wound healing and Transwell assays, Annexin V/propdidum iodide double staining and western blot analysis, respectively. In vivo, tumor growth was assessed by subcutaneous inoculation of cells into BALB/c nude mice. Phenotypic EMT markers and regulatory factors were detected in the tumor tissues derived from the mice. In vitro, silencing of CXCR4 expression suppressed cell migration and invasion, and induced apoptosis. The protein expression of the EMT‑associated markers N‑cadherin and matrix metalloproteinases 2/9 were attenuated, while E‑cadherin was increased. In vivo, CXCR4 siRNA inhibited tumor growth, and EMT‑associated proteins had similar expression patterns to the experimental results observed in vitro. In conclusion, the present study demonstrated that CXCR4 silencing suppressed EMT in OSCC, thus affecting tumor metastasis.
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September-2016
Volume 12 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Duan Y, Zhang S, Wang L, Zhou X, He Q, Liu S, Yue K and Wang X: Targeted silencing of CXCR4 inhibits epithelial-mesenchymal transition in oral squamous cell carcinoma. Oncol Lett 12: 2055-2061, 2016
APA
Duan, Y., Zhang, S., Wang, L., Zhou, X., He, Q., Liu, S. ... Wang, X. (2016). Targeted silencing of CXCR4 inhibits epithelial-mesenchymal transition in oral squamous cell carcinoma. Oncology Letters, 12, 2055-2061. https://doi.org/10.3892/ol.2016.4838
MLA
Duan, Y., Zhang, S., Wang, L., Zhou, X., He, Q., Liu, S., Yue, K., Wang, X."Targeted silencing of CXCR4 inhibits epithelial-mesenchymal transition in oral squamous cell carcinoma". Oncology Letters 12.3 (2016): 2055-2061.
Chicago
Duan, Y., Zhang, S., Wang, L., Zhou, X., He, Q., Liu, S., Yue, K., Wang, X."Targeted silencing of CXCR4 inhibits epithelial-mesenchymal transition in oral squamous cell carcinoma". Oncology Letters 12, no. 3 (2016): 2055-2061. https://doi.org/10.3892/ol.2016.4838