Open Access

PAQR3 gene expression and its methylation level in colorectal cancer tissues

  • Authors:
    • Ri-Heng Li
    • Ai-Min Zhang
    • Shuang Li
    • Tian-Yang Li
    • Lian-Jing Wang
    • Hao-Ran Zhang
    • Jian-Wei Shi
    • Xiao-Rui Liu
    • Yuan Chen
    • Ya-Chao Chen
    • Teng-Yao Wei
    • Ying Gao
    • Wei Li
    • Hong-Ying Tang
    • Mei-Yu Tang
  • View Affiliations

  • Published online on: July 11, 2016     https://doi.org/10.3892/ol.2016.4843
  • Pages: 1773-1778
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the PAQR3 gene expression and its methylation level in colorectal cancer tissues, as well as the association with colorectal cancer clinical data. In total, 54 cases of colorectal cancer tissue samples and normal adjacent tissue samples were collected between June, 2013 and July, 2014. RT-PCR and western blot analysis were used to detect the mRNA and protein levels of PAQR3 in colorectal samples, respectively. MSP was used to detect the methylation level of PAQR3 gene in colorectal samples, which was compared with colorectal data. The results showed that a decreased expression level of PAQR3 mRNA in colorectal cancer tissues and the expression reduction rate was 57.4% (31/54). Similarly, the expression level of PAQR3 protein was reduced in cancer tissues, and the reduction rate was 46.3% (25/54), while the protein expression reduction rate in cancer adjacent tissue was 5.6% (3/54), and the difference was statistically significant (P<0.05). Furthermore, the methylation rates of PAQR3 in cancer tissues and cancer adjacent tissues were 33.3% (18/54) and 5.6% (3/54), respectively. In addition, PAQR3 mRNA and protein levels in colorectal cancer tissues were associated with the differentiation degree, lymphatic metastasis and tumor infiltration depth. The methylation level of PAQR3 was associated with age, differentiated degree, lymphatic metastasis and tumor infiltration depth. In conclusion, the expression of PAQR3 mRNA and protein in colorectal cancer was reduced and methylation of PAQR3 occurred. Although the PAQR3 mRNA and protein levels were not associated with gender, age or the location of tumor, there was an association with differentiation degree, lymphatic metastasis and tumor infiltration depth. In addition, the methylation level of PAQR3 was not correlated with gender or tumor location, but was correlated with age, differentiation degree, lymphatic metastasis and tumor infiltration depth.
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September-2016
Volume 12 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Li R, Zhang A, Li S, Li T, Wang L, Zhang H, Shi J, Liu X, Chen Y, Chen Y, Chen Y, et al: PAQR3 gene expression and its methylation level in colorectal cancer tissues. Oncol Lett 12: 1773-1778, 2016.
APA
Li, R., Zhang, A., Li, S., Li, T., Wang, L., Zhang, H. ... Tang, M. (2016). PAQR3 gene expression and its methylation level in colorectal cancer tissues. Oncology Letters, 12, 1773-1778. https://doi.org/10.3892/ol.2016.4843
MLA
Li, R., Zhang, A., Li, S., Li, T., Wang, L., Zhang, H., Shi, J., Liu, X., Chen, Y., Chen, Y., Wei, T., Gao, Y., Li, W., Tang, H., Tang, M."PAQR3 gene expression and its methylation level in colorectal cancer tissues". Oncology Letters 12.3 (2016): 1773-1778.
Chicago
Li, R., Zhang, A., Li, S., Li, T., Wang, L., Zhang, H., Shi, J., Liu, X., Chen, Y., Chen, Y., Wei, T., Gao, Y., Li, W., Tang, H., Tang, M."PAQR3 gene expression and its methylation level in colorectal cancer tissues". Oncology Letters 12, no. 3 (2016): 1773-1778. https://doi.org/10.3892/ol.2016.4843