Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
September-2016 Volume 12 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September-2016 Volume 12 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Daphnetin protects oxidative stress‑induced neuronal apoptosis via regulation of MAPK signaling and HSP70 expression

  • Authors:
    • Zhilin Qi
    • Shimei Qi
    • Lin Gui
    • Lei Shen
    • Zunyong Feng
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry, Wannan Medical College, Wuhu, Anhui 241002, P.R. China, Department of Microbiology and Immunology, Wannan Medical College, Wuhu, Anhui 241002, P.R. China, Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Science, Nanjing Normal University, Nanjing, Jiangsu 210046, P.R. China, Anhui Province Key Laboratory of Active Biological Macromolecules, Wannan Medical College, Wuhu, Anhui 241002, P.R. China
  • Pages: 1959-1964
    |
    Published online on: July 12, 2016
       https://doi.org/10.3892/ol.2016.4849
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Neurodegenerative disorders are characterized by progressive degeneration and loss of neurons in the brain. Oxidative stress is implicated in the pathogenesis of neurological disorders, although the pathological mechanism remains unelucidated. Daphnetin, an active ingredient extracted from Changbai daphne (Daphne Korean Nakai), exhibits various pharmacological effects, including anti‑inflammatory, anti‑oxidative and anti‑tumor effects. However, the neuroprotective effects, as well as the specific mechanisms of daphnetin, remain unclear. Neuronal-like rat pheochromocytoma PC12 cells were pretreated with daphnetin for 2 h, then treated with or without H2O2 for various times. Cell morphology was detected using an inverted microscope, the apoptotic ratio was determined by Annexin V fluorescein isothiocyanate/propidium iodide assay, nuclear morphology was observed and photographed using a fluorescence microscope following 4',6‑diamidino‑2‑phenylindole staining. The levels of pro-caspase 3, cleavage of poly ADP‑ribose polymerase and caspase 3 were detected by western blotting. In addition, the activation of mitogen‑activated protein kinase (MAPK) signal pathway and the expression of HSP70 were detected by western blotting. The present study demonstrated that daphnetin attenuated hydrogen peroxide (H2O2)‑induced apoptosis in a concentration‑dependent manner, reduced the cleavage of poly ADP ribose polymerase and caspase 3, and inhibited the phosphorylation of p38 MAPK and c‑Jun N‑terminal kinases (JNK) in H2O2‑induced PC12 cells. In addition, daphnetin induced the expression of HSP70 in a dose‑ and time‑dependent manner, and daphnetin‑induced HSP70 expression was reduced by extracellular signal‑regulated kinase (ERK) 1/2 inhibitor U0126 in PC12 cells. Therefore, the present results indicate that daphnetin protects PC12 cells against oxidative stress injury by regulating p38 MAPK and JNK signaling and increasing the expression of HSP70 via ERK signaling. This suggests that daphnetin may have the potential to treat certain neurodegenerative diseases. The present results not only provide insight into the potential use of daphnetin in H2O2‑induced PC12 cell apoptosis, but also highlight the potential role of HSP70 in neuroprotection.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Gong QH, Shi XR, Hong ZY, Pan LL, Liu XH and Zhu YZ: A new hope for neurodegeneration: Possible role of hydrogen sulfide. J Alzheimers Dis. 24(Suppl 2): S173–S182. 2011.

2 

Pan LL, Liu XH, Jia YL, Wu D, Xiong QH, Gong QH, Wang Y and Zhu YZ: A novel compound derived from danshensu inhibits apoptosis via upregulation of heme oxygenase-1 expression in SH-SY5Y cells. Biochim Biophys Acta. 1830:2861–2871. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Chong ZZ, Li F and Maiese K: Oxidative stress in the brain: Novel cellular targets that govern survival during neurodegenerative disease. Prog Neurobiol. 75:207–246. 2005. View Article : Google Scholar : PubMed/NCBI

4 

Asaithambi A, Kanthasamy A, Saminathan H, Anantharam V and Kanthasamy AG: Protein kinase D1 (PKD1) activation mediates a compensatory protective response during early stages of oxidative stress-induced neuronal degeneration. Mol Neurodegener. 6:432011. View Article : Google Scholar : PubMed/NCBI

5 

Karuppagounder SS, Pinto JT, Xu H, Chen H-L, Beal MF and Gibson GE: Dietary supplementation with resveratrol reduces plaque pathology in a transgenic model of Alzheimer's disease. Neurochem Int. 54:111–118. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Yin W, Zhang X, Shi X and Li Y: Curcumin protects SH-SY5Y cells from oxidative stress by up-regulating HO-1 via Phosphatidylinositol 3 Kinase/Akt/Nrf-2 and down-regulating HO-2. Mol Neurodegener. 7(Suppl 1): S142012. View Article : Google Scholar

7 

Zhang L, Ding W, Sun H, Zhou Q, Huang J, Li X, Xie Y and Chen J: Salidroside protects PC12 cells from MPP+-induced apoptosis via activation of the PI3K/Akt pathway. Food Chem Toxicol. 50:2591–2597. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Ouyang X and Yu T: Effect of Daphnetin on the expression of β-APP after the ischemia in the front brain of the mouse. Zhonghua Naoxueguanbing Zazhi. 6:2012.(In Chinese).

9 

Kuang NZ, Fu YY, Gong SQ, Zeng ZP, Zhang ZQ and Chen ZY: Effects of Daphnetin extract on the expression of Bcl-2 and Bax mRNA during the apoptosis of SMMC-7721 cells. Guangdong Yiyao Zazhi. 33:1374–1377. 2012.(In Chinese).

10 

Gao Y, Han C, Huang H, Xin Y, Xu Y, Luo L and Yin Z: Heat shock protein 70 together with its co-chaperone CHIP inhibits TNF-alpha induced apoptosis by promoting proteasomal degradation of apoptosis signal-regulating kinase1. Apoptosis. 15:822–833. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Sabirzhanov B, Stoica BA, Hanscom M, Piao CS and Faden AI: Over-expression of HSP70 attenuates caspase-dependent and caspase-independent pathways and inhibits neuronal apoptosis. J Neurochem. 123:542–554. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Chow AM, Tang DW, Hanif A and Brown IR: Induction of heat shock proteins in cerebral cortical cultures by celastrol. Cell Stress Chaperones. 18:155–160. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Kou X, Shen K, An Y, Qi S, Dai WX and Yin Z: Ampelopsin inhibits H2O2-induced apoptosis by ERK and Akt signaling pathways and up-regulation of heme oxygenase-1. Phytother Res. 26:988–994. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Wang Y, Li CF, Pan LM and Gao ZL: 7,8-Dihydroxycoumarin inhibits A549 human lung adenocarcinoma cell proliferation by inducing apoptosis via suppression of Akt/NF-kB signaling. Exp Ther Med. 5:1770–1774. 2013.PubMed/NCBI

15 

Du JS, Zhao Q, Zhang YL, Wang Y and Ma M: 7,8-dihydroxycoumarin may promote sciatic nerve regeneration by suppressing NF-kB expression in mice. Mol Med Rep. 8:1525–1530. 2013.PubMed/NCBI

16 

Du G, Tu H, Li X, Pei A, Chen J, Miao Z, Li J, Wang C, Xie H, Xu X and Zhao H: Daphnetin, a natural coumarin derivative, provides the neuroprotection against glutamate-induced toxicity in HT22 cells and ischemic brain injury. Neurochem Res. 39:269–275. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Yang L, Yang Q, Zhang K, Li YJ, Wu YM, Liu SB, Zheng LH and Zhao MG: Neuroprotective effects of daphnetin against NMDA receptor-mediated excitotoxicity. Molecules. 19:14542–14555. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Yu S, Shen Y, Liu J and Ding F: Involvement of ERK1/2 pathway in neuroprotection by salidroside against hydrogen peroxide-induced apoptotic cell death. J Mol Neurosci. 40:321–331. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Takayama S, Reed JC and Homma S: Heat-shock proteins as regulators of apoptosis. Oncogene. 22:9041–9047. 2003. View Article : Google Scholar : PubMed/NCBI

20 

Gao Y, Han C, Huang H, Xin Y, Xu Y, Luo L and Yin Z: Heat shock protein 70 together with its co-chaperone CHIP inhibits TNF-alpha induced apoptosis by promoting proteasomal degradation of apoptosis signal-regulating kinase1. Apoptosis. 15:822–833. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Turturici G, Sconzo G and Geraci F: Hsp70 and its molecular role in nervous system diseases. Biochem Res Int. 2011:6181272011. View Article : Google Scholar : PubMed/NCBI

22 

Sabirzhanov B, Stoica BA, Hanscom M, Piao CS and Faden AI: Over-expression of HSP70 attenuates caspase-dependent and caspase-independent pathways and inhibits neuronal apoptosis. J Neurochem. 123:542–554. 2012. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Qi Z, Qi S, Gui L, Shen L and Feng Z: Daphnetin protects oxidative stress‑induced neuronal apoptosis via regulation of MAPK signaling and HSP70 expression. Oncol Lett 12: 1959-1964, 2016.
APA
Qi, Z., Qi, S., Gui, L., Shen, L., & Feng, Z. (2016). Daphnetin protects oxidative stress‑induced neuronal apoptosis via regulation of MAPK signaling and HSP70 expression. Oncology Letters, 12, 1959-1964. https://doi.org/10.3892/ol.2016.4849
MLA
Qi, Z., Qi, S., Gui, L., Shen, L., Feng, Z."Daphnetin protects oxidative stress‑induced neuronal apoptosis via regulation of MAPK signaling and HSP70 expression". Oncology Letters 12.3 (2016): 1959-1964.
Chicago
Qi, Z., Qi, S., Gui, L., Shen, L., Feng, Z."Daphnetin protects oxidative stress‑induced neuronal apoptosis via regulation of MAPK signaling and HSP70 expression". Oncology Letters 12, no. 3 (2016): 1959-1964. https://doi.org/10.3892/ol.2016.4849
Copy and paste a formatted citation
x
Spandidos Publications style
Qi Z, Qi S, Gui L, Shen L and Feng Z: Daphnetin protects oxidative stress‑induced neuronal apoptosis via regulation of MAPK signaling and HSP70 expression. Oncol Lett 12: 1959-1964, 2016.
APA
Qi, Z., Qi, S., Gui, L., Shen, L., & Feng, Z. (2016). Daphnetin protects oxidative stress‑induced neuronal apoptosis via regulation of MAPK signaling and HSP70 expression. Oncology Letters, 12, 1959-1964. https://doi.org/10.3892/ol.2016.4849
MLA
Qi, Z., Qi, S., Gui, L., Shen, L., Feng, Z."Daphnetin protects oxidative stress‑induced neuronal apoptosis via regulation of MAPK signaling and HSP70 expression". Oncology Letters 12.3 (2016): 1959-1964.
Chicago
Qi, Z., Qi, S., Gui, L., Shen, L., Feng, Z."Daphnetin protects oxidative stress‑induced neuronal apoptosis via regulation of MAPK signaling and HSP70 expression". Oncology Letters 12, no. 3 (2016): 1959-1964. https://doi.org/10.3892/ol.2016.4849
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team