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Article

lncRNA uc009yby.1 promotes renal cell proliferation and is associated with poor survival in patients with clear cell renal cell carcinomas

  • Authors:
    • Xingfeng Ren
    • Tianbiao Lan
    • Yan Chen
    • Ziyun Shao
    • Cheng Yang
    • Jun Peng
  • View Affiliations / Copyright

    Affiliations: Nephrology Department, Wuhan General Hospital of Guangzhou Military Region, Wuhan, Hubei 430030, P.R. China
  • Pages: 1929-1934
    |
    Published online on: July 13, 2016
       https://doi.org/10.3892/ol.2016.4856
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Abstract

The expression and function of long non-coding RNAs (lncRNAs) in clear cell renal cell carcinoma (ccRCC) remains unclear. The present study measured the expression profiles of three lncRNAs (uc009yby.1, ENST00000514034, and ENST00000450687) using reverse transcription-quantitative polymerase chain reaction, and assessed their signatures in distinguishing ccRCC from matched normal tissues via analysis of receiver operating characteristic (ROC) curves. The expression of uc009yby.1 was inhibited by transfection of renal cells with small interfering RNA, and then the cell proliferation was evaluated by using a Cell Counting Kit-8. The results showed that the expressions of uc009yby.1 and ENST00000514034 were markedly increased in ccRCC compared with the matched normal tissues (P<0.0001 and P=0.0008, respectively), whereas the ENST00000450687 expression was not significantly altered. ROC curves yielded an area under the curve (AUC) value of 0.7000 for uc009yby.1, with sensitivity of 54.29% and specificity of 82.86%; and an AUC value of 0.6627 for ENST00000514034, with sensitivity of 60.00% and specificity of 67.14%. Furthermore, knockdown of uc009yby.1 suppressed renal cell proliferation (Day 0, P=0.7844; Day 1, P=0.0018; Day 2, P=0.0001; Day 3, P<0.000; Day 4, P<0.0001). Taken together, these findings suggest that the expression profiles of uc009yby.1 and ENST00000514034 may serve as novel biomarkers for ccRCC detection, and that uc009yby.1 is strongly associated with renal cell proliferation.
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Copy and paste a formatted citation
Spandidos Publications style
Ren X, Lan T, Chen Y, Shao Z, Yang C and Peng J: lncRNA uc009yby.1 promotes renal cell proliferation and is associated with poor survival in patients with clear cell renal cell carcinomas. Oncol Lett 12: 1929-1934, 2016.
APA
Ren, X., Lan, T., Chen, Y., Shao, Z., Yang, C., & Peng, J. (2016). lncRNA uc009yby.1 promotes renal cell proliferation and is associated with poor survival in patients with clear cell renal cell carcinomas. Oncology Letters, 12, 1929-1934. https://doi.org/10.3892/ol.2016.4856
MLA
Ren, X., Lan, T., Chen, Y., Shao, Z., Yang, C., Peng, J."lncRNA uc009yby.1 promotes renal cell proliferation and is associated with poor survival in patients with clear cell renal cell carcinomas". Oncology Letters 12.3 (2016): 1929-1934.
Chicago
Ren, X., Lan, T., Chen, Y., Shao, Z., Yang, C., Peng, J."lncRNA uc009yby.1 promotes renal cell proliferation and is associated with poor survival in patients with clear cell renal cell carcinomas". Oncology Letters 12, no. 3 (2016): 1929-1934. https://doi.org/10.3892/ol.2016.4856
Copy and paste a formatted citation
x
Spandidos Publications style
Ren X, Lan T, Chen Y, Shao Z, Yang C and Peng J: lncRNA uc009yby.1 promotes renal cell proliferation and is associated with poor survival in patients with clear cell renal cell carcinomas. Oncol Lett 12: 1929-1934, 2016.
APA
Ren, X., Lan, T., Chen, Y., Shao, Z., Yang, C., & Peng, J. (2016). lncRNA uc009yby.1 promotes renal cell proliferation and is associated with poor survival in patients with clear cell renal cell carcinomas. Oncology Letters, 12, 1929-1934. https://doi.org/10.3892/ol.2016.4856
MLA
Ren, X., Lan, T., Chen, Y., Shao, Z., Yang, C., Peng, J."lncRNA uc009yby.1 promotes renal cell proliferation and is associated with poor survival in patients with clear cell renal cell carcinomas". Oncology Letters 12.3 (2016): 1929-1934.
Chicago
Ren, X., Lan, T., Chen, Y., Shao, Z., Yang, C., Peng, J."lncRNA uc009yby.1 promotes renal cell proliferation and is associated with poor survival in patients with clear cell renal cell carcinomas". Oncology Letters 12, no. 3 (2016): 1929-1934. https://doi.org/10.3892/ol.2016.4856
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