Gliomatosis peritonei with bilateral ovarian teratomas: A report of two cases

Introduction

Gliomatosis peritonei (GP) is a rare condition that is typically associated with immature and, less commonly, mature teratomas and ventriculoperitoneal shunting. GP occurs commonly in young girls and, in rare cases, can be found in males. GP is characterized as a myriad of peritoneal nodular or miliary implants composed of benign, mature glia (1). As GP has an extremely low incidence rate, there is no widely accepted guidance as to the method by which these patients should be treated. Approximately 100 cases of GP have been described in the literature. Since the disease was first described by Neuhaüser in 1906 (2), it has been well-documented that GP, when benign, does not adversely affect patient prognosis. Glial implants associated with the disease may remain stable for long periods of time, or may completely disappear. The repeated measurement of tumor markers and imaging are usually undertaken throughout follow-up. To the best of our knowledge, only a few cases have been reported that describe the findings of a radiological analysis (3–5). The current study presents two cases of GP with bilateral ovarian teratomas, describing the diagnosis, associated radiological observations and complete clinical course of each patient.

Case report

Case 1

A 19-year-old woman was referred to The Second Affiliated Hospital, Zhejiang University School of Medicine (Hangzhou, China) on January 16, 2011, presenting with a 15-day history of lower abdominal distension. During a physical examination of the pelvis and abdomen, a large firm mass was identified and was palpable extending up to 20 cm to the xiphoid process. Analysis of serum tumor markers demonstrated an increase in carcinoembryonic antigen (13.4 ng/ml; normal range, <5 ng/ml), cancer antigen (CA)125 (241.7 units/ml; normal range, <35 units/ml) and CA19-9 (2,215.6 units/ml; normal range, <37 units/ml), whilst the α-fetoprotein level was normal (1.2 ng/ml; normal range, <20 ng/ml).

Contrast-enhanced computed tomography (CT) revealed two cystic, solid masses in the pelvis and lower abdomen, each containing dispersed regions of calcified and fatty components. The two masses demonstrated similarities with bilateral ovarian teratomas. Additionally, multiple nodules throughout the peritoneum were associated with omental caking, and ascites was also detected (Fig. 1A-C). Based on the results from the CT scan and the elevated serum tumor markers, it was suspected that the patient was presenting with malignant germ cell tumors, specifically immature teratomas, with lymphadenopathy.

Figure 1. (A) Axial image of contrast-enhanced lower abdominal computed tomography demonstrated a mixed solid and cystic mass with scattered areas of fatty components and calcifications suggestive of an ovarian teratoma. Ascites was also observed. (B) Left ovarian teratoma and (C and D) multiple nodules throughout the peritoneum were exhibited. (A-C) Case 1. (D) Case 2.

A right salpingo-oophorectomy, left tumor resection and omentectomy were performed. The right ovarian mass was 30×25×20 cm in size, and consisted of a yellow-white solid component and cystic areas. Two cystic masses, measuring 1×1 and 2×1 cm, were observed in the left ovary. All tumors contained fatty components. Numerous miliary nodules were identified in the retroperitoneum and peritoneum, suggesting the presence of GP implants. The largest implant was detected in the greater omentum, measuring 1 cm in diameter, and had adhered to the right ovary. Following histological examination, the right ovarian mass was confirmed as an immature teratoma, and the left mass was confirmed as a mature teratoma. Mature teratoma was characterized by well-differentiated tissue, while immature teratoma contained immature tissue, typically the neuroectodermal structures. Extensive peritoneal implants were observed, with numerous lymphocytes and foam cells. The implants had no envelope, but were without cellular atypia or mitosis (Fig. 2).

Figure 2. Case 1: (A) Multiple nodules demonstrating a variety of sizes in the greater omentum, with numerous lymphocytes and foam cells (staining, hematoxylin and eosin; magnification, ×40). (B) Microscopic view of the omental implants exhibiting mature glial tissues, consistent with gliomatosis peritonei (staining, hematoxylin and eosin; magnification, ×100).

Following surgery, a chemotherapy regimen (30 mg bleomycin on days 1–3, 90 mg etoposide on day 1 and 0.17 mg cisplatin on day 1, for 6 cycles; interval time, 4 weeks) was administered. Currently, 4 years after the first surgery, the patient is alive and well, with a good prognosis and without recurrence or metastasis. A long-term follow-up is planned at an outpatient clinic.

Case 2

A 15-year-old woman was referred to The Second Affiliated Hospital, Zhejiang University School of Medicine on July 17, 2013, presenting with progressively increasing fullness of the abdomen and a significant increase in weight over the last 3 years. The patient was subsequently referred to the Department of Endocrinology for the appropriate treatment. Contrast-enhanced CT detected a tumor in the lower abdomen and pelvis, measuring 10×12×9 cm; a second tumor in the left ovary, with a diameter of 3 cm, was also revealed on the CT scan (Fig. 1D). The patient was treated with a tumorectomy. During the surgery, bilateral tumors were found, and the larger tumor was located in the right ovary. The tumors were removed. Intraoperatively, tiny gray spots, measuring up to 0.5 cm in diameter, were removed from the greater omentum and peritoneum. During microscopic examination, the tumors appeared to consist of a teratomatous component, exhibiting similarities to skin, intestinal mucosa, cartilage, bones and neuroglial tissue. It was also observed that the peritoneal implants consisted of mature glial tissue that appeared to be well-demarcated, without mitosis or cellular atypia. Therefore, a diagnosis of bilateral mature teratoma and GP was confirmed. No chemotherapy was administered for case 2. During a follow-up period of 25 months after surgery, no notable events were recorded and the patient has a good prognosis. A long-term follow-up is planned at an outpatient clinic.

Discussion

GP is a rare complication associated with ovarian teratomas, with only ~100 cases recorded to date. Typical symptoms of the disease are similar to those experienced in the present two cases, and affected patients may occasionally develop Pseudo-Meigs' syndrome associated with ascites and pleural effusions owing to ovarian teratoma (6). The pre-operative diagnosis of teratoma may be confirmed through the combination of imaging and clinical findings. However, small GP lesions may be easily overlooked or cause a delay in accurate diagnosis due to the rareness of the disease.

GP is characterized by a myriad of peritoneal nodular or miliary implants composed of mature glia (7). As mature glial cells are not aggressive, the implants may remain stable for long periods of time, exhibiting benign behavior. However, on rare occasions, GP may develop a secondary malignant glial tumor (8). The pathogenesis underlying the development of GP is not yet fully understood. These glial nodules are traditionally confined as peritoneal seedlings via capsular rupture from the ovarian teratoma, although genetic evidence has demonstrated that they may have a different genetic identity compared with ovarian tumors (9–11). In the majority of cases, a benign clinical course is expected for patients with GP, particularly when undergoing cisplatin-based chemotherapy (12). In the present two cases, following surgical resection, each patient demonstrated a positive prognosis within a 19-month follow-up period. However, doctors should conduct long-term follow-ups in patients presenting with GP due to the potential for malignant transformation.

When comparing the radiological data from the present cases with previously published cases, notable findings have been observed: i) Although the teratomas in the present cases were localized on each ovary (bilateral), the majority of cases within the literature reported unilateral teratomas, without a particular preference as to which ovary was affected; up until now, only one case of bilateral teratoma had been reported in the literature (13). ii) Although the radiological data regarding GP in the current literature is scarce, the radiological findings in the present two cases appear to be consistent with previous observations. The CT scans from the current study revealed multiple nodules throughout the peritoneum, with associated omental caking and ascites. The peritoneal implants were miliary, measured 0.3–1.2 cm in size, and were well-marginated and markedly enhanced (4,5,14). The findings in the two present cases are similar to previous observations of peritoneal tuberculosis and peritoneal dissemination of malignant tumors (5). Therefore, with regard to the identification of ovarian masses, imaging analysis of peritoneal dissemination alone cannot aid the differentiation of benign implants from diffuse peritoneal malignant seeding. However, radiological analysis may provide important information regarding tumor staging and the requirement for surgery. Therefore, in patients with bilateral ovarian teratomas, GP should be considered as a possible diagnosis by radiologists and relative radiological investigations are warranted.

In summary, the current study reports two cases presenting with bilateral ovarian teratomas with GP. These cases suggest that: i) In patients with ovarian teratoma, a possible diagnosis of GP should be considered, particularly in individuals presenting with bilateral teratomas; ii) although radiology may provide biological and histopathological information regarding GP status, the radiological differential diagnosis of GP is challenging due to the rareness of the disease; and iii) the follow-up of patients with GP should not be discontinued following the initial diagnosis and treatment, even if symptoms are alleviated or disappear.

References