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September-2016 Volume 12 Issue 3

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

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Article

Spheres from cervical cancer cells display stemness and cancer drug resistance

  • Authors:
    • Huan Liu
    • Haijuan Wang
    • Chunxiao Li
    • Tingting Zhang
    • Xiting Meng
    • Ying Zhang
    • Haili Qian
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Molecular Oncology, Cancer Institute/Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, P.R. China, Guangdong Medical College, Dongguan, Guangdong 150080, P.R. China, Department of Gynecology, Minimally Invasive Center, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100006, P.R. China
  • Pages: 2184-2188
    |
    Published online on: July 20, 2016
       https://doi.org/10.3892/ol.2016.4893
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Abstract

Cervical cancer is one of the most common gynecological malignant tumors and is the cause of a serious health problem worldwide. An increasing amount of evidence has shown that cancer stem cells (CSCs) are present in tumors, and that these CSCs may be responsible for tumor metastasis and relapse. The present study aimed to identify and characterize a CSC population from the CaSki cell line. First, a stem cell culture medium was used to selectively expand the cancer stem‑like cell spheres, and the putative stemness markers, Oct4 and Sox2, were identified. These markers were all highly expressed in the CaSki sphere‑forming cells. Next, target region amplified polymorphism-polymerase chain reaction was performed and the CaSki sphere‑forming cells were found to exhibit higher telomerase activity than the CaSki control cells cultured in non‑stem cell medium. Using the 3‑(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay, it was found that the CaSki sphere‑forming cells were more resistant to chemotherapeutic drugs than the control CaSki cells. Using the tumor invasive assay, it was shown that the CaSki sphere‑forming cells were more invasive than the control CaSki cells. These characteristics all suggested that the tumor sphere‑forming cells mirrored the acknowledged CSC phenotypes. Overall, the use of a suspended sphere culture of CaSki cells may be an easy and feasible approach for enriching cancer stem‑like cells in cervical cancer research.
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Copy and paste a formatted citation
Spandidos Publications style
Liu H, Wang H, Li C, Zhang T, Meng X, Zhang Y and Qian H: Spheres from cervical cancer cells display stemness and cancer drug resistance. Oncol Lett 12: 2184-2188, 2016.
APA
Liu, H., Wang, H., Li, C., Zhang, T., Meng, X., Zhang, Y., & Qian, H. (2016). Spheres from cervical cancer cells display stemness and cancer drug resistance. Oncology Letters, 12, 2184-2188. https://doi.org/10.3892/ol.2016.4893
MLA
Liu, H., Wang, H., Li, C., Zhang, T., Meng, X., Zhang, Y., Qian, H."Spheres from cervical cancer cells display stemness and cancer drug resistance". Oncology Letters 12.3 (2016): 2184-2188.
Chicago
Liu, H., Wang, H., Li, C., Zhang, T., Meng, X., Zhang, Y., Qian, H."Spheres from cervical cancer cells display stemness and cancer drug resistance". Oncology Letters 12, no. 3 (2016): 2184-2188. https://doi.org/10.3892/ol.2016.4893
Copy and paste a formatted citation
x
Spandidos Publications style
Liu H, Wang H, Li C, Zhang T, Meng X, Zhang Y and Qian H: Spheres from cervical cancer cells display stemness and cancer drug resistance. Oncol Lett 12: 2184-2188, 2016.
APA
Liu, H., Wang, H., Li, C., Zhang, T., Meng, X., Zhang, Y., & Qian, H. (2016). Spheres from cervical cancer cells display stemness and cancer drug resistance. Oncology Letters, 12, 2184-2188. https://doi.org/10.3892/ol.2016.4893
MLA
Liu, H., Wang, H., Li, C., Zhang, T., Meng, X., Zhang, Y., Qian, H."Spheres from cervical cancer cells display stemness and cancer drug resistance". Oncology Letters 12.3 (2016): 2184-2188.
Chicago
Liu, H., Wang, H., Li, C., Zhang, T., Meng, X., Zhang, Y., Qian, H."Spheres from cervical cancer cells display stemness and cancer drug resistance". Oncology Letters 12, no. 3 (2016): 2184-2188. https://doi.org/10.3892/ol.2016.4893
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