miR-429 promotes the proliferation of non‑small cell lung cancer cells via targeting DLC-1

Retraction in: /10.3892/ol.2021.12806

  • Authors:
    • Peng Xiao
    • Wenliang Liu
    • Hui Zhou
  • View Affiliations

  • Published online on: July 22, 2016     https://doi.org/10.3892/ol.2016.4904
  • Pages: 2163-2168
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Abstract

The microRNA (miR)-200 family has been demonstrated to be associated with the tumorigenesis and progression of multiple types of human cancer, including non-small cell lung cancer (NSCLC). As a member of the miR-200 family, miR-429 was recently identified to have an oncogenic role in NSCLC. However, the role of miR‑429 in NSCLC growth as well as the underlying mechanism remains to be fully elucidated. In the present study, NSCLC cell line H1229 was transfected with miR‑429 mimic or inhibitor, respectively. It was observed that overexpression of miR‑429 led to a significant increase in NSCLC cell proliferation, while knockdown of miR‑429 suppressed the proliferation of H1229 cells. Bioinformatic prediction suggested that deleted in liver cancer 1 (DLC‑1), a tumor suppressor in NSCLC, was a putative target gene of miR‑429. Therefore, a luciferase reporter assay was performed and confirmed that miR‑429 was able to bind the 3'‑untranslated region of DLC‑1 mRNA in H1229 cells. Furthermore, overexpression of miR‑429 inhibited the protein expression of DLC‑1, while knockdown of miR‑429 promoted the protein expression of DLC‑1 in NSCLC H1229 cells. In addition, overexpression of DLC‑1 not only inhibited H1229 cell proliferation, but also additionally reversed the promoting effect of miR‑429 overexpression on H1229 cell proliferation. Based on these findings, the present study suggests that miR-429 may have an oncogenic role in the regulation of cell proliferation via direct inhibition of DLC-1 protein expression in NSCLC cells. Therefore, miR‑429 may present a putative therapeutic target for the treatment of NSCLC growth.
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September-2016
Volume 12 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Xiao P, Liu W and Zhou H: miR-429 promotes the proliferation of non‑small cell lung cancer cells via targeting DLC-1 Retraction in /10.3892/ol.2021.12806. Oncol Lett 12: 2163-2168, 2016
APA
Xiao, P., Liu, W., & Zhou, H. (2016). miR-429 promotes the proliferation of non‑small cell lung cancer cells via targeting DLC-1 Retraction in /10.3892/ol.2021.12806. Oncology Letters, 12, 2163-2168. https://doi.org/10.3892/ol.2016.4904
MLA
Xiao, P., Liu, W., Zhou, H."miR-429 promotes the proliferation of non‑small cell lung cancer cells via targeting DLC-1 Retraction in /10.3892/ol.2021.12806". Oncology Letters 12.3 (2016): 2163-2168.
Chicago
Xiao, P., Liu, W., Zhou, H."miR-429 promotes the proliferation of non‑small cell lung cancer cells via targeting DLC-1 Retraction in /10.3892/ol.2021.12806". Oncology Letters 12, no. 3 (2016): 2163-2168. https://doi.org/10.3892/ol.2016.4904